##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511764_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2175746 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.865434200499507 31.0 31.0 34.0 27.0 34.0 2 31.053400075192602 31.0 31.0 34.0 27.0 34.0 3 31.159586183313678 31.0 31.0 34.0 27.0 34.0 4 34.7326144687845 37.0 35.0 37.0 32.0 37.0 5 34.35416771994525 37.0 35.0 37.0 30.0 37.0 6 34.30361264596143 37.0 35.0 37.0 30.0 37.0 7 34.12557945642552 37.0 35.0 37.0 30.0 37.0 8 34.03037992486255 37.0 35.0 37.0 30.0 37.0 9 35.524722554930584 39.0 35.0 39.0 30.0 39.0 10 35.32538081191463 39.0 35.0 39.0 30.0 39.0 11 35.32677251848332 39.0 35.0 39.0 30.0 39.0 12 35.14291787736252 38.0 35.0 39.0 29.0 39.0 13 35.12352039254582 38.0 35.0 39.0 29.0 39.0 14 36.12638285902858 39.0 36.0 41.0 28.0 41.0 15 36.0339083698189 39.0 36.0 41.0 27.0 41.0 16 35.874338272941785 39.0 36.0 41.0 27.0 41.0 17 35.84285068201895 39.0 36.0 41.0 27.0 41.0 18 35.7674659633983 39.0 36.0 41.0 27.0 41.0 19 35.78360019965566 39.0 36.0 41.0 27.0 41.0 20 35.88278319252339 39.0 36.0 41.0 27.0 41.0 21 35.83093384981519 39.0 36.0 41.0 27.0 41.0 22 35.72111496470636 39.0 36.0 41.0 27.0 41.0 23 35.61465768522613 39.0 36.0 41.0 27.0 41.0 24 35.4426991937478 39.0 36.0 41.0 27.0 41.0 25 34.90967144142745 39.0 35.0 40.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20583163937996574 1101 2 0.3713875546712089 1101 3 0.3617119527401158 1101 4 0.3080432433844891 1101 5 0.26717065717393496 1101 6 0.25801373385443327 1101 7 0.44368640576279716 1101 8 0.280336502491231 1101 9 0.2478892317833541 1101 10 0.45480250955261425 1101 11 0.46094822995016926 1101 12 0.48937780750333815 1101 13 0.38081275138517867 1101 14 0.5315969904819724 1101 15 0.4030664719010133 1101 16 0.3282117310211774 1101 17 0.314705712392076 1101 18 0.5162338816319689 1101 19 0.3478209288077352 1101 20 -0.09002304249096937 1101 21 0.26458384871917673 1101 22 0.20865529336472122 1101 23 0.24983354304241345 1101 24 -0.1115254559152703 1101 25 0.1416547059255393 1102 1 0.5968294243390879 1102 2 0.5539687193332803 1102 3 0.5205421543409372 1102 4 0.6142993962758112 1102 5 0.7991993992019246 1102 6 0.7297746378479246 1102 7 0.8172327183082757 1102 8 0.8428052030005801 1102 9 0.8134303651994372 1102 10 0.9242970094028067 1102 11 0.9210432519935736 1102 12 0.9515241500107194 1102 13 0.9649270560549539 1102 14 1.094427909028397 1102 15 1.0742187746874663 1102 16 1.0272253957902606 1102 17 1.2438709523013358 1102 18 1.1547895037695355 1102 19 1.3692511817718227 1102 20 1.2093771843634613 1102 21 0.9174708923351531 1102 22 1.1824316705423072 1102 23 1.112545684034579 1102 24 1.0948759587147023 1102 25 1.4078504534750849 1103 1 0.38973335172519086 1103 2 0.597101031847945 1103 3 0.5226374307498034 1103 4 0.6150306477425076 1103 5 0.7343862140465376 1103 6 0.6943560854324886 1103 7 0.5529366473705934 1103 8 0.602691764735809 1103 9 0.6848453472638738 1103 10 0.8342252671385708 1103 11 0.5543866426536681 1103 12 0.6700070022685907 1103 13 0.6824188425714297 1103 14 1.0258694172921636 1103 15 0.8172551235426084 1103 16 0.978234690436409 1103 17 1.1561354432329765 1103 18 0.8634245569057484 1103 19 1.0288316236237094 1103 20 1.0331958675241424 1103 21 1.0528425105590244 1103 22 1.2111600053842366 1103 23 1.1313979335233313 1103 24 1.0762919046062862 1103 25 1.1780248223778287 1104 1 0.19912714386514097 1104 2 0.2827982518766987 1104 3 0.34315212025556363 1104 4 0.40603977933051283 1104 5 0.3976310661391125 1104 6 0.5021817146211731 1104 7 0.4358599703777415 1104 8 0.5783239905350754 1104 9 0.6521882778759505 1104 10 0.6247595966451343 1104 11 0.6843771263646161 1104 12 0.7104309833170035 1104 13 0.5788067022162906 1104 14 0.745068024269429 1104 15 0.7689933648514042 1104 16 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1.0819418145391921 1107 15 1.0150247220292599 1107 16 1.2363755732988722 1107 17 1.0647493990608012 1107 18 1.0812991301982393 1107 19 1.121750157683664 1107 20 0.9180557291141085 1107 21 1.0122813757850793 1107 22 1.0610626654740756 1107 23 1.1382530578709336 1107 24 1.0805219073416197 1107 25 1.0451648625962946 1108 1 0.28827322337405903 1108 2 0.36780219676681014 1108 3 0.22166387055542103 1108 4 0.32413838790164107 1108 5 0.4157775606595919 1108 6 0.5122443768063221 1108 7 0.5477806073976836 1108 8 0.41927309005119895 1108 9 0.5242557195349065 1108 10 0.4979391324706697 1108 11 0.5706872175303417 1108 12 0.4326605531187582 1108 13 0.5443434124971773 1108 14 0.55216745830311 1108 15 0.6103798227049282 1108 16 0.7598538494755758 1108 17 0.833721987934986 1108 18 0.8300250981545361 1108 19 0.7888177862677495 1108 20 0.786791554865836 1108 21 0.8502585326912637 1108 22 0.9579020098133242 1108 23 0.7329539060520815 1108 24 0.8356935826094087 1108 25 1.0226562296906252 1109 1 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-0.954867681724636 2315 4 -0.9190760833195881 2315 5 -1.2086578682254512 2315 6 -1.0093503808376099 2315 7 -1.1549289489397196 2315 8 -1.249717780207348 2315 9 -1.4324245603075596 2315 10 -1.548216568752558 2315 11 -1.343643578441423 2315 12 -1.3425799408757229 2315 13 -1.4198112218861496 2315 14 -1.4663888357022827 2315 15 -1.512237638188111 2315 16 -1.5078769386559046 2315 17 -1.7435194258938935 2315 18 -1.734523615967369 2315 19 -1.556399074842858 2315 20 -1.6021013397284207 2315 21 -1.6969780089553979 2315 22 -1.8290630476798242 2315 23 -1.7021389664213444 2315 24 -1.8574681416390817 2315 25 -1.838205341532138 2316 1 -0.9979592441629741 2316 2 -1.137918247254131 2316 3 -1.1636463076788353 2316 4 -1.2449125296408425 2316 5 -1.3192573020870242 2316 6 -1.3715768826125938 2316 7 -1.4628589473155529 2316 8 -2.323047787864141 2316 9 -1.8711319549106875 2316 10 -1.9139147288026734 2316 11 -1.8316723030058029 2316 12 -1.9317351416924424 2316 13 -1.9579252152502633 2316 14 -1.9058653896826527 2316 15 -1.9901669404921094 2316 16 -1.9257944077387137 2316 17 -1.9297853485371093 2316 18 -1.9383183675857367 2316 19 -1.8745387068528814 2316 20 -1.9535920935742155 2316 21 -1.994586422143641 2316 22 -2.196893507783308 2316 23 -2.0610707281791605 2316 24 -2.316772301828408 2316 25 -2.1112342981204577 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35279.0 3 1.0 4 602.0 5 5433.0 6 6996.0 7 5779.0 8 4803.0 9 4143.0 10 3762.0 11 3693.0 12 3534.0 13 3511.0 14 3305.0 15 3364.0 16 3503.0 17 3615.0 18 3971.0 19 4271.0 20 5339.0 21 6913.0 22 8763.0 23 11211.0 24 13606.0 25 16011.0 26 18850.0 27 21248.0 28 24864.0 29 30311.0 30 38310.0 31 49572.0 32 64064.0 33 83120.0 34 109321.0 35 147222.0 36 218773.0 37 394510.0 38 814173.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.99397848112425 19.58821414378442 13.985515306963148 24.432292068128184 2 15.060305752601636 21.0872041129801 40.42553680438801 23.42695333003025 3 18.87403217103467 25.020337851936763 30.177143839400372 25.928486137628198 4 12.822130892117004 17.7952297740637 38.18731598265606 31.195323351163236 5 13.421063036835626 37.704125122872775 35.63995450144229 13.234857338849313 6 31.308847632030577 37.673699043914134 18.203089882734474 12.814363441320817 7 27.582033932269667 31.869161198044253 22.277140805958048 18.27166406372803 8 24.97713427946093 36.76394211456668 20.811988164059592 17.446935441912796 9 26.125522004866376 15.639233623777777 20.27244908183216 37.96279528952369 10 14.795477045574254 29.655759449862256 34.22256090554688 21.326202599016614 11 34.52360707545826 23.006591762089876 23.92797688700795 18.541824275443915 12 22.561043430621037 25.04455942927162 31.90818229701445 20.4862148430929 13 28.751701715181827 21.826444814789962 26.024774950752523 23.397078519275688 14 21.228213219741644 21.899017624299898 28.04353081655671 28.82923833940175 15 23.422127399062205 29.686369640573858 24.10483576667497 22.78666719368897 16 22.583564441805247 28.45621685619553 26.964222845865283 21.995995856133945 17 21.83168439698384 28.053780174707892 27.758387238216226 22.356148190092043 18 21.519975217695446 28.032867807179702 29.055735366168662 21.391421608956193 19 23.089735658482194 27.193293702481814 27.823514325661176 21.893456313374816 20 23.027917780843904 27.363901852514033 28.26483422237706 21.343346144265002 21 23.354150714283744 26.785249748821784 27.61098951807794 22.249610018816533 22 22.80647649128161 27.25019372665743 27.990123847177014 21.953205934883943 23 23.06647926734095 26.903737844399117 27.589617538076595 22.44016535018334 24 22.697318528909165 27.01579136535239 28.182563589683724 22.104326516054723 25 23.047305716461697 26.97822630795896 27.952028290678076 22.022439684901265 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 186.0 1 186.0 2 426.0 3 666.0 4 666.0 5 666.0 6 1529.5 7 2393.0 8 2393.0 9 2393.0 10 3461.0 11 4529.0 12 4529.0 13 4529.0 14 7555.5 15 10582.0 16 10582.0 17 10582.0 18 18992.5 19 27403.0 20 27403.0 21 27403.0 22 42682.5 23 57962.0 24 57962.0 25 57962.0 26 81652.5 27 105343.0 28 105343.0 29 105343.0 30 135369.5 31 165396.0 32 165396.0 33 165396.0 34 184017.5 35 202639.0 36 202639.0 37 202639.0 38 219941.0 39 237243.0 40 237243.0 41 237243.0 42 252613.0 43 267983.0 44 267983.0 45 267983.0 46 273854.5 47 279726.0 48 279726.0 49 279726.0 50 272569.0 51 265412.0 52 265412.0 53 265412.0 54 239342.0 55 213272.0 56 213272.0 57 213272.0 58 182868.5 59 152465.0 60 152465.0 61 152465.0 62 124238.0 63 96011.0 64 96011.0 65 96011.0 66 73562.5 67 51114.0 68 51114.0 69 51114.0 70 37053.5 71 22993.0 72 22993.0 73 22993.0 74 15798.5 75 8604.0 76 8604.0 77 8604.0 78 5701.5 79 2799.0 80 2799.0 81 2799.0 82 1798.0 83 797.0 84 797.0 85 797.0 86 495.0 87 193.0 88 193.0 89 193.0 90 111.0 91 29.0 92 29.0 93 29.0 94 17.0 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42206213409101984 2 0.0 3 0.0 4 0.0 5 0.033643633034370735 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.002160178623791564 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2175746.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.4382400358562 #Duplication Level Percentage of deduplicated Percentage of total 1 79.84830156567384 55.44525530572692 2 13.365107017144059 18.5609901832271 3 3.8259707709060087 7.970060302810235 4 1.3365727524563022 3.712370384417828 5 0.6204898610748825 2.154286195656638 6 0.2963306148931193 1.2346005820152768 7 0.1846099966261632 0.8973295281122287 8 0.11246666346726368 0.6247589739097371 9 0.06938344739897243 0.4336078027504544 >10 0.30405124189711047 3.649145110958682 >50 0.02028938992644273 0.9810041614542884 >100 0.01377977073357387 1.9147034160905823 >500 0.0013896265961925187 0.6310197433040704 >1k 0.0011911085110218047 1.4679376494087342 >5k 6.617269505676693E-5 0.3229306601572787 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7028 0.32301564612781086 No Hit TATCAACGCAGAGTACTTTTTTTTT 4695 0.2157880561425828 No Hit GGTATCAACGCAGAGTACTTTTTTT 4323 0.19869047214150914 No Hit TTGTAGAACAGTGTATATCAATGAG 2198 0.10102282159774165 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.596124731471413E-5 2 0.0 0.0 0.0 0.0 4.596124731471413E-5 3 0.0 0.0 0.0 0.0 4.596124731471413E-5 4 0.0 0.0 0.0 0.0 4.596124731471413E-5 5 4.596124731471413E-5 0.0 0.0 0.0 4.596124731471413E-5 6 4.596124731471413E-5 0.0 0.0 0.0 4.596124731471413E-5 7 4.596124731471413E-5 0.0 0.0 0.0 4.596124731471413E-5 8 4.596124731471413E-5 0.0 0.0 0.0 4.596124731471413E-5 9 4.596124731471413E-5 0.0 0.0 0.0 4.596124731471413E-5 10 4.596124731471413E-5 4.596124731471413E-5 0.0 0.0 9.192249462942826E-5 11 4.596124731471413E-5 4.596124731471413E-5 0.0 0.0 9.192249462942826E-5 12 4.596124731471413E-5 4.596124731471413E-5 0.0 0.0 9.192249462942826E-5 13 4.596124731471413E-5 4.596124731471413E-5 0.0 0.0 9.192249462942826E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 315 0.0 12.059523 18 TCGGAAC 65 8.015347E-4 11.694104 3 CCAACGA 205 0.0 11.581554 19 GCGACTA 110 4.992271E-7 11.223578 16 CGACTAA 110 4.992271E-7 11.223578 17 AGCATCG 60 0.0058702193 11.085037 5 TAACCGC 60 0.005891349 11.079686 6 GGTATCA 1560 0.0 11.066623 1 CGGTTTC 250 0.0 11.016376 13 TAGGACC 295 0.0 10.950835 4 GTGTTCG 90 9.54997E-5 10.552083 9 TATACTG 370 0.0 10.528645 5 GTCCTAA 580 0.0 10.524781 1 GTATTAG 200 1.8189894E-12 10.491892 1 GTACTAA 200 1.8189894E-12 10.491892 1 CGCCCTA 175 2.3646862E-10 10.310893 16 GCCGGTT 260 0.0 10.227403 11 TAAGCGA 130 3.860332E-7 10.227403 13 CCTATAC 140 9.670839E-8 10.180136 3 GTCCTAC 1005 0.0 10.154975 1 >>END_MODULE