FastQCFastQC Report
Thu 26 May 2016
SRR1511763_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511763_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372824
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44210.1863180750026129No Hit
TATCAACGCAGAGTACTTTTTTTTT30580.12887597225921518No Hit
GGTATCAACGCAGAGTACTTTTTTT28070.11829785942825934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG551.3956295E-414.3826431
GGTATCA12500.011.9447841
AGGACCG1251.9623258E-811.3476065
GCGACTA1002.5249488E-510.40197216
GGCGAGG6300.010.35694319
TAGGACC4300.010.3359984
CGTGCCA1106.3900625E-610.31600610
GTATCAA24300.010.2135991
TGGCGAG11750.09.97945518
TTAGGAC9100.09.9759183
TATGGCG11900.09.93312816
GACGTGA7100.09.8559027
TTAGACA2500.09.8345924
ATAATAC2750.09.6282723
ATGGCGA12700.09.53079917
ACTATCG2700.09.45633911
TAGGACG13100.09.4563394
CGACTAA1107.192115E-59.45633917
TCGAACT1201.7963268E-59.45633819
GTATTAG1802.2015229E-89.3387291
Produced by FastQC (version 0.11.5)