FastQCFastQC Report
Thu 26 May 2016
SRR1511763_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511763_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372824
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56100.23642714335323647No Hit
TATCAACGCAGAGTACTTTTTTTTT42570.17940647936804416No Hit
GGTATCAACGCAGAGTACTTTTTTT37220.15685950580405458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.012.9491
CGCCCTA1001.93712E-611.39690316
TCCAACG1451.45046215E-810.4799118
GAGCCGT1002.4070412E-510.4471626
TAGGACC3550.010.4370834
CTAATAC1553.6616257E-910.4198183
GACGTGA6050.010.2038387
GTCTTAG3350.09.961511
TAGGACT3250.09.9389034
TTAGGAC7850.09.9240063
GGCGAGG4750.09.79733819
GTTAGAC800.00451527359.50042253
CTAAGAC2303.6379788E-129.5004223
CTAGGAC2800.09.5004223
GTATCAA28450.09.4843181
TATACTG3650.09.1099945
GCCGGTT1151.1142364E-49.08448711
GTCTATA2106.2391337E-109.080561
GGACGTG11550.08.9629326
TTAGGGT2252.6193447E-108.8670614
Produced by FastQC (version 0.11.5)