##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511763_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2372824 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.795205206960144 31.0 31.0 34.0 27.0 34.0 2 30.98533435265321 31.0 31.0 34.0 27.0 34.0 3 31.080821417854843 31.0 31.0 34.0 27.0 34.0 4 34.666568611915594 37.0 35.0 37.0 32.0 37.0 5 34.27113388940773 37.0 35.0 37.0 30.0 37.0 6 34.21968717443856 37.0 35.0 37.0 30.0 37.0 7 34.03563391132254 37.0 35.0 37.0 30.0 37.0 8 33.93762116364299 37.0 35.0 37.0 30.0 37.0 9 35.415685276278396 39.0 35.0 39.0 30.0 39.0 10 35.21347853865268 38.0 35.0 39.0 29.0 39.0 11 35.207659312279375 39.0 35.0 39.0 29.0 39.0 12 35.02938271022208 38.0 35.0 39.0 28.0 39.0 13 35.00278233868167 38.0 35.0 39.0 28.0 39.0 14 35.9914894657168 39.0 36.0 41.0 27.0 41.0 15 35.898050171441284 39.0 36.0 41.0 27.0 41.0 16 35.726782938810466 39.0 36.0 41.0 27.0 41.0 17 35.68386951581744 39.0 36.0 41.0 27.0 41.0 18 35.59872750781347 39.0 36.0 41.0 27.0 41.0 19 35.61227128518592 39.0 36.0 41.0 27.0 41.0 20 35.716975637468266 39.0 36.0 41.0 27.0 41.0 21 35.654384817415874 39.0 36.0 41.0 27.0 41.0 22 35.54050532192864 39.0 36.0 41.0 27.0 41.0 23 35.42554820753667 39.0 36.0 41.0 27.0 41.0 24 35.24750676830646 39.0 35.0 41.0 26.0 41.0 25 34.69643555527085 39.0 34.0 40.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3091959857478166 1101 2 0.48620464082308956 1101 3 0.44077441136206374 1101 4 0.49435283756903914 1101 5 0.5824754091472784 1101 6 0.5097971875471075 1101 7 0.5602957004861722 1101 8 0.38979208361419637 1101 9 0.41998556175551016 1101 10 0.5120175547723065 1101 11 0.613713547511324 1101 12 0.7045473113167304 1101 13 0.6939428194881643 1101 14 0.5610108082560714 1101 15 0.5292009817795531 1101 16 0.169795525539854 1101 17 0.21568508730430125 1101 18 0.3417220658554996 1101 19 0.34959689903301694 1101 20 0.3202919075710611 1101 21 0.3034918263983428 1101 22 0.20248827368796896 1101 23 0.3627337737022245 1101 24 -0.05338939817819721 1101 25 0.17660949584710295 1102 1 0.5535039090631422 1102 2 0.49278885792603333 1102 3 0.34962324930641486 1102 4 0.49646899204334005 1102 5 0.5892356296862928 1102 6 0.643605477590377 1102 7 0.6796158552825986 1102 8 0.6369255873078856 1102 9 0.6403295239933584 1102 10 0.76199092694619 1102 11 0.6091666838362713 1102 12 0.8623440253996932 1102 13 0.8511835965184389 1102 14 0.9420982973343328 1102 15 0.7113309827234318 1102 16 0.8447388277539858 1102 17 0.9432150988127148 1102 18 0.9947980776912431 1102 19 1.0586281484106195 1102 20 1.0748463482698156 1102 21 0.7998760706919796 1102 22 0.8387748999380733 1102 23 1.033706715501161 1102 24 1.0391858113589763 1102 25 1.1859465539009548 1103 1 0.4798905447858566 1103 2 0.478789419776394 1103 3 0.5411836410494075 1103 4 0.656255311746321 1103 5 0.7613354146477675 1103 6 0.6804343047319676 1103 7 0.641998033321677 1103 8 0.6432525201026564 1103 9 0.6081511474709416 1103 10 0.8224594392828521 1103 11 0.6292515687961426 1103 12 0.8546829089034773 1103 13 0.8101598731040411 1103 14 1.0142775090397151 1103 15 1.0005392377056381 1103 16 0.8869588031371762 1103 17 1.1833061590648413 1103 18 1.019233928468509 1103 19 1.1563068037491675 1103 20 1.0685930212467483 1103 21 1.0787383912014619 1103 22 1.3460781110056814 1103 23 1.0712842546471961 1103 24 1.0727006915355304 1103 25 1.0819958010130364 1104 1 0.22419036848836882 1104 2 0.27434986884345847 1104 3 0.4101418474026701 1104 4 0.4461360651723254 1104 5 0.48518161638715185 1104 6 0.5612901340183214 1104 7 0.5986503143752628 1104 8 0.6885983318903399 1104 9 0.8527180389332898 1104 10 0.7584344496051045 1104 11 0.7800424498248262 1104 12 0.858243266217201 1104 13 0.5925184910365715 1104 14 0.9600430923615804 1104 15 1.0946445770946909 1104 16 1.0166242966718428 1104 17 1.023203133170739 1104 18 1.0900806151898337 1104 19 0.9980380254448988 1104 20 0.8565854581499437 1104 21 0.969085775903558 1104 22 1.0270607565166472 1104 23 1.0558598626769253 1104 24 1.082148008733384 1104 25 1.1261668423479207 1105 1 0.40518799393164784 1105 2 0.37354370372139556 1105 3 0.40411352723015526 1105 4 0.4212164635493991 1105 5 0.3786451971688223 1105 6 0.41473215058412904 1105 7 0.505292373321609 1105 8 0.483289546030484 1105 9 0.4480446495496153 1105 10 0.4909314424565068 1105 11 0.5564656257476983 1105 12 0.5781390599486187 1105 13 0.553518266669009 1105 14 0.6216438302815703 1105 15 0.3357859535896921 1105 16 0.6045748236239987 1105 17 0.7818054478961045 1105 18 0.7811560837129363 1105 19 0.5302106201934578 1105 20 0.7480196031537147 1105 21 0.820351115477628 1105 22 0.8757069164034661 1105 23 0.9686509951722044 1105 24 0.8685033446303265 1105 25 1.0634296203923057 1106 1 0.35121944334423816 1106 2 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1.242234466757374 1107 15 1.0993127395460363 1107 16 1.3058083726247602 1107 17 1.380179150773884 1107 18 1.2959188973091997 1107 19 1.274529114482128 1107 20 1.2505294189520981 1107 21 1.1933568759899629 1107 22 1.2649225897683962 1107 23 1.2429770957848163 1107 24 1.3395373476868002 1107 25 1.206529592430158 1108 1 0.16970221431974153 1108 2 0.0760191586869432 1108 3 0.11013176016287929 1108 4 0.25913254650573947 1108 5 0.25849333879818204 1108 6 0.29628962965633576 1108 7 0.3099344125834449 1108 8 0.4408479347052747 1108 9 0.4015766232485234 1108 10 0.47869396609996784 1108 11 0.4040863871712048 1108 12 0.3657063402441878 1108 13 0.33207949584433294 1108 14 0.42665544121986443 1108 15 0.5706044966341182 1108 16 0.6981782952981987 1108 17 0.44932745246747885 1108 18 0.4616923039272862 1108 19 0.6700519799544153 1108 20 0.6030119051125737 1108 21 0.5832397680028691 1108 22 0.6318206357071148 1108 23 0.38788543976346546 1108 24 0.6258858354375718 1108 25 0.49538244078083693 1109 1 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-1.2480075217421174 2315 7 -1.3266709174111142 2315 8 -1.3482126123053675 2315 9 -1.5450281490340103 2315 10 -1.6555035524006456 2315 11 -1.4043105068439061 2315 12 -1.4812290625705344 2315 13 -1.5453969469012137 2315 14 -1.7292309964545964 2315 15 -1.7383685731693959 2315 16 -1.7987835418507956 2315 17 -1.8944397053454196 2315 18 -1.8426663447197598 2315 19 -1.8594609398929407 2315 20 -1.8177713322826392 2315 21 -2.076398004875209 2315 22 -2.02325544791443 2315 23 -2.0319252208031173 2315 24 -1.9524894962453203 2315 25 -1.8637210378985287 2316 1 -1.346476354727102 2316 2 -1.4046347974350795 2316 3 -1.4282924448183998 2316 4 -1.393210558414438 2316 5 -1.6329065778939693 2316 6 -1.690562963763547 2316 7 -1.7309471031438122 2316 8 -2.4480039719592206 2316 9 -2.1285604523447006 2316 10 -2.0657394238206876 2316 11 -2.207632321313177 2316 12 -2.1285943577564055 2316 13 -2.3222407781472825 2316 14 -2.241332650002086 2316 15 -2.3765889514605263 2316 16 -2.4329868660614693 2316 17 -2.309714437955442 2316 18 -2.2427919739355744 2316 19 -2.1759695430980273 2316 20 -2.276439491438083 2316 21 -2.224344377973445 2316 22 -2.4104279176581542 2316 23 -2.3683547837195675 2316 24 -2.4942680567809745 2316 25 -2.460637816245125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39839.0 3 3.0 4 716.0 5 6326.0 6 7826.0 7 6224.0 8 5282.0 9 4803.0 10 4501.0 11 4142.0 12 4083.0 13 3951.0 14 3819.0 15 3934.0 16 3896.0 17 4095.0 18 4325.0 19 4980.0 20 6085.0 21 7783.0 22 10092.0 23 12685.0 24 15265.0 25 18421.0 26 21100.0 27 23969.0 28 28039.0 29 34182.0 30 43126.0 31 55450.0 32 71288.0 33 93122.0 34 122127.0 35 165447.0 36 246575.0 37 442556.0 38 842767.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.84471699598253 20.268773204941475 14.805376818205286 24.081132980870706 2 15.441853251652882 21.287208827961955 39.47363984855177 23.797298071833392 3 18.119885840669177 25.568773748074026 30.34873214363982 25.962608267616982 4 13.337651675809079 18.322640027241803 37.486218952606684 30.85348934434244 5 14.00206739784424 37.41370384610196 35.14846142431233 13.435767331741472 6 31.548610432126445 37.11590071577159 18.293012882539962 13.042475969562005 7 27.910540351918222 31.76021483262138 22.59413256103276 17.735112254427634 8 25.545299609241983 35.878261514549756 20.88186903031999 17.694569845888275 9 26.351469809813118 15.698467311524158 20.560985559822388 37.389077318840336 10 15.56318547014022 29.305334066074856 33.61193244842433 21.519548015360602 11 33.85611406492854 22.83603840824267 24.397047568635514 18.910799958193277 12 23.103820595206386 24.997892806208974 31.318631301773753 20.579655296810888 13 28.509531258955572 22.080651578035287 26.08132756580345 23.328489597205692 14 21.57732726911056 21.824374669170574 27.84357373323938 28.75472432847948 15 23.373246393327108 29.94794388458647 23.725232044180267 22.953577677906157 16 22.971109530247503 28.030987549013325 26.790819715242257 22.20708320549691 17 22.153855490335566 28.125937701237007 27.561336196869217 22.15887061155821 18 22.014401405245394 27.58249242253113 28.790757342306044 21.612348829917433 19 23.537523221275578 27.065007771330702 27.57941591959623 21.81805308779749 20 23.70112574721092 26.94864853019019 27.77268773410923 21.577537988489663 21 23.61317147837345 26.535975698155447 27.262409685674115 22.588443137796986 22 23.576885601291963 27.126959268786898 27.361953520362235 21.934201609558908 23 23.194851366978757 26.90047808012731 27.38951561514887 22.515154937745066 24 23.17344227806192 26.810079466492247 27.78512017747629 22.231358077969542 25 23.34226382762081 26.869282260515035 27.47018357033491 22.318270341529242 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 187.0 1 187.0 2 432.5 3 678.0 4 678.0 5 678.0 6 1513.5 7 2349.0 8 2349.0 9 2349.0 10 3187.0 11 4025.0 12 4025.0 13 4025.0 14 6820.0 15 9615.0 16 9615.0 17 9615.0 18 18131.0 19 26647.0 20 26647.0 21 26647.0 22 43085.0 23 59523.0 24 59523.0 25 59523.0 26 85032.5 27 110542.0 28 110542.0 29 110542.0 30 140671.0 31 170800.0 32 170800.0 33 170800.0 34 195769.0 35 220738.0 36 220738.0 37 220738.0 38 241413.0 39 262088.0 40 262088.0 41 262088.0 42 278284.5 43 294481.0 44 294481.0 45 294481.0 46 301485.5 47 308490.0 48 308490.0 49 308490.0 50 300696.5 51 292903.0 52 292903.0 53 292903.0 54 264550.5 55 236198.0 56 236198.0 57 236198.0 58 202790.0 59 169382.0 60 169382.0 61 169382.0 62 138291.5 63 107201.0 64 107201.0 65 107201.0 66 82382.5 67 57564.0 68 57564.0 69 57564.0 70 41553.0 71 25542.0 72 25542.0 73 25542.0 74 17510.0 75 9478.0 76 9478.0 77 9478.0 78 6383.5 79 3289.0 80 3289.0 81 3289.0 82 2069.5 83 850.0 84 850.0 85 850.0 86 525.5 87 201.0 88 201.0 89 201.0 90 120.5 91 40.0 92 40.0 93 40.0 94 26.5 95 13.0 96 13.0 97 13.0 98 6.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40635125066165884 2 0.0 3 0.0 4 0.0 5 0.03202934562361136 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0023179131701297692 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2372824.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.02942035565182 #Duplication Level Percentage of deduplicated Percentage of total 1 79.6238294300777 54.96386792055555 2 13.308435020520065 18.37347110614715 3 3.8073602945076868 7.884596226449683 4 1.4170443288532568 3.912709945560159 5 0.657100666612845 2.2679639065798556 6 0.3439188526224539 1.4244311427545306 7 0.22333132727157787 1.079150244817078 8 0.13329078884016757 0.7360788713907482 9 0.09197165056100938 0.5713874754641134 >10 0.357903579101568 4.085283203140204 >50 0.02034414151509166 0.9647592682161803 >100 0.01302822641964584 1.7225234463280743 >500 0.0016481428404599705 0.7658429701058392 >1k 7.325079290877495E-4 1.0115447780056794 >5k 6.104232742397913E-5 0.2363894944852063 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5610 0.23642714335323647 No Hit TATCAACGCAGAGTACTTTTTTTTT 4257 0.17940647936804416 No Hit GGTATCAACGCAGAGTACTTTTTTT 3722 0.15685950580405458 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.214387582054126E-5 2 0.0 0.0 0.0 0.0 4.214387582054126E-5 3 0.0 0.0 0.0 0.0 4.214387582054126E-5 4 0.0 0.0 0.0 4.214387582054126E-5 4.214387582054126E-5 5 0.0 0.0 0.0 8.428775164108252E-5 4.214387582054126E-5 6 0.0 0.0 0.0 8.428775164108252E-5 4.214387582054126E-5 7 0.0 0.0 0.0 8.428775164108252E-5 4.214387582054126E-5 8 0.0 0.0 0.0 8.428775164108252E-5 4.214387582054126E-5 9 0.0 0.0 0.0 1.264316274616238E-4 4.214387582054126E-5 10 0.0 0.0 0.0 1.6857550328216504E-4 4.214387582054126E-5 11 0.0 4.214387582054126E-5 0.0 2.528632549232476E-4 4.214387582054126E-5 12 0.0 4.214387582054126E-5 0.0 2.528632549232476E-4 8.428775164108252E-5 13 0.0 4.214387582054126E-5 0.0 2.528632549232476E-4 1.6857550328216504E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1480 0.0 12.949 1 CGCCCTA 100 1.93712E-6 11.396903 16 TCCAACG 145 1.45046215E-8 10.47991 18 GAGCCGT 100 2.4070412E-5 10.447162 6 TAGGACC 355 0.0 10.437083 4 CTAATAC 155 3.6616257E-9 10.419818 3 GACGTGA 605 0.0 10.203838 7 GTCTTAG 335 0.0 9.96151 1 TAGGACT 325 0.0 9.938903 4 TTAGGAC 785 0.0 9.924006 3 GGCGAGG 475 0.0 9.797338 19 GTTAGAC 80 0.0045152735 9.5004225 3 CTAAGAC 230 3.6379788E-12 9.500422 3 CTAGGAC 280 0.0 9.500422 3 GTATCAA 2845 0.0 9.484318 1 TATACTG 365 0.0 9.109994 5 GCCGGTT 115 1.1142364E-4 9.084487 11 GTCTATA 210 6.2391337E-10 9.08056 1 GGACGTG 1155 0.0 8.962932 6 TTAGGGT 225 2.6193447E-10 8.867061 4 >>END_MODULE