FastQCFastQC Report
Thu 26 May 2016
SRR1511762_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511762_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173799
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53660.24684894969590104No Hit
TATCAACGCAGAGTACTTTTTTTTT34020.15650021000101666No Hit
GGTATCAACGCAGAGTACTTTTTTT33650.1547981207094124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG450.00884530813.1813922
ACCGTGC752.1648742E-411.347798
GGTATCA11700.011.3224761
TTGCGGA600.006080111511.03282818
CTAATAC1553.952664E-910.3716363
ACCGTCC750.002751059810.0869258
GCCTCGA1701.6880222E-910.01298616
GTCCTAA4850.09.9879611
GTATCAA22400.09.8419091
TAATACT3400.09.7346244
GTAATAC2151.0913936E-119.6764113
TTAGACT2107.2759576E-119.4564914
GTTAGAC1502.8314753E-79.4564913
GTTGCAC1002.8859585E-49.4564913
CGTTTAA850.00536065879.3045111
TAGGACC2850.09.2905894
TAGACTG2600.09.0927815
CCCCGTA1252.9068928E-59.07844215
CCTGGAC4600.09.045343
ATCGCGC1151.1677602E-49.045348
Produced by FastQC (version 0.11.5)