FastQCFastQC Report
Thu 26 May 2016
SRR1511762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511762_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173799
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69270.3186587168362852No Hit
TATCAACGCAGAGTACTTTTTTTTT47810.21993753792323945No Hit
GGTATCAACGCAGAGTACTTTTTTT41630.19150804651212003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGG400.00529057114.2457058
GTCCGCC550.003076060712.08726411
GCCGGTT951.044189E-611.99638411
TAGGACG6300.011.7624184
TCCAACG1657.2759576E-1211.51168118
GGTATCA14100.011.2974961
ACCGTGC1104.9904884E-711.2238888
TTAGGAC4600.010.946133
GACCCGA909.547299E-510.5523736
GGACGTG7150.010.3605126
TGCCGGT1106.0694365E-610.36051210
AGGACGT7250.010.3521755
CTATACC1856.002665E-1110.2707194
CTATTAG2150.010.2040881
GTCCTAG2450.010.12261
GACGTGA3250.09.9354657
CGTGAAA3750.09.8770229
GTATCAA27700.09.6763461
GTAGGAC6350.09.575223
GTATTAG1701.5585101E-89.5386041
Produced by FastQC (version 0.11.5)