FastQCFastQC Report
Thu 26 May 2016
SRR1511761_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511761_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916369
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59150.3086566313690109No Hit
TATCAACGCAGAGTACTTTTTTTTT35770.18665507530125983No Hit
GGTATCAACGCAGAGTACTTTTTTT34800.18159341963891087No Hit
GTCCTACAGTGGACATTTCTAAATT31360.16364280574357026No Hit
CTGTAGGACGTGGAATATGGCAAGA26270.13708215902052268No Hit
GTCCTAAAGTGTGTATTTCTCATTT25330.13217704940958658No Hit
CTTTAGGACGTGAAATATGGCGAGG22840.11918372714231967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTA1300.014.54871216
GGTATCA10950.013.0898021
TCCAACG2300.012.74593618
TAGGACC4700.012.2735414
ATAAACG550.003175781612.0357523
ACGAAAT803.0044861E-511.82082812
GATCTAC958.752639E-611.4458551
TGTCGAG752.164258E-411.34799518
TAGACGA600.00607989511.0327735
GTATTAA1852.7284841E-1110.6865481
AGTGCGG803.9342564E-410.63874610
CGAAATC909.9682184E-510.50740213
GTAAGAC1451.5501428E-810.43493753
GTCCTAC14550.010.3945961
GTATCAA21800.010.2478121
GATATAC6300.010.1988681
AGGACCT9950.010.1694765
TAATACA2700.010.1571564
TTAGGAC10750.010.1164293
CCAACGT1701.6880222E-910.01293719
Produced by FastQC (version 0.11.5)