##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511761_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1916369 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.580322474429508 31.0 30.0 34.0 25.0 34.0 2 26.90193224791259 31.0 26.0 34.0 10.0 34.0 3 28.5827891183796 31.0 28.0 34.0 16.0 34.0 4 33.11361225317253 35.0 33.0 37.0 28.0 37.0 5 33.5630768395857 35.0 35.0 37.0 28.0 37.0 6 33.821833373426514 37.0 35.0 37.0 30.0 37.0 7 33.87243114452384 37.0 35.0 37.0 30.0 37.0 8 33.90170890887924 37.0 35.0 37.0 30.0 37.0 9 35.51689105803736 39.0 35.0 39.0 30.0 39.0 10 35.39036062470224 39.0 35.0 39.0 30.0 39.0 11 35.40640189859051 39.0 35.0 39.0 30.0 39.0 12 35.31372454887342 39.0 35.0 39.0 30.0 39.0 13 35.30488752427116 39.0 35.0 39.0 30.0 39.0 14 36.46436985778835 40.0 37.0 41.0 30.0 41.0 15 36.39591957498791 40.0 36.0 41.0 29.0 41.0 16 36.33987191402073 40.0 36.0 41.0 29.0 41.0 17 36.3171977839341 40.0 36.0 41.0 29.0 41.0 18 36.26447829201996 40.0 36.0 41.0 29.0 41.0 19 36.294284660208966 40.0 37.0 41.0 29.0 41.0 20 36.22437589002953 40.0 37.0 41.0 29.0 41.0 21 36.14009619233039 40.0 36.0 41.0 28.0 41.0 22 36.03971312414259 40.0 36.0 41.0 28.0 41.0 23 35.93302594646438 40.0 36.0 41.0 27.0 41.0 24 35.823963443366075 40.0 36.0 41.0 27.0 41.0 25 35.70921831860148 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0753584350478071 1101 2 3.4673253816199363 1101 3 2.137113890031447 1101 4 1.1657224199977918 1101 5 0.7742299201826981 1101 6 0.5558889152642834 1101 7 0.3279693206436676 1101 8 -0.026909220468759543 1101 9 0.043665009676523425 1101 10 0.21072728840662336 1101 11 0.06376743374773497 1101 12 0.21908807641795391 1101 13 0.2594468830363823 1101 14 0.2099649293641832 1101 15 0.061219292965212446 1101 16 0.249274474724551 1101 17 0.23168713788690098 1101 18 0.2076122418204278 1101 19 0.4169640482490209 1101 20 0.49588339167840445 1101 21 0.5117911013727792 1101 22 0.45946478410572666 1101 23 0.4245086367614732 1101 24 0.4269986858453976 1101 25 0.2086840883388419 1102 1 1.5782547635396327 1102 2 4.241312759289581 1102 3 2.8172085724087594 1102 4 1.8564448648135894 1102 5 1.3714934153848404 1102 6 1.1170568235141616 1102 7 0.9607959882550858 1102 8 1.0031725323140819 1102 9 1.135083367978929 1102 10 0.9770827287528547 1102 11 1.0511821869944526 1102 12 0.9646346506051842 1102 13 1.107849457300425 1102 14 1.234512285949151 1102 15 1.2955181132762945 1102 16 1.2962087370903603 1102 17 1.368558070072588 1102 18 1.318531607174343 1102 19 1.3258390477109785 1102 20 1.375778551043524 1102 21 1.287820063664391 1102 22 1.1438926189234522 1102 23 1.0763052212956978 1102 24 1.0158107623846249 1102 25 0.935526952874767 1103 1 1.175150149014815 1103 2 3.806463413070137 1103 3 2.477189044107174 1103 4 1.4521897506790111 1103 5 0.8902116778799041 1103 6 0.7623322914064872 1103 7 0.6373254107890745 1103 8 0.44085283988483326 1103 9 0.6240074806135425 1103 10 0.4592882063129693 1103 11 0.6101316414546432 1103 12 0.7580586235225155 1103 13 0.7620568748431396 1103 14 0.8105901165090188 1103 15 0.5190747694400741 1103 16 0.584094766325002 1103 17 0.7487178938039705 1103 18 0.9631903918587597 1103 19 0.8741699881849883 1103 20 0.8451971665254803 1103 21 0.8271509623838043 1103 22 1.0232279500534318 1103 23 0.8544123250365843 1103 24 1.05835708589278 1103 25 0.7713472539574937 1104 1 1.2728187153681567 1104 2 3.7781883038998316 1104 3 2.326640725453238 1104 4 1.4331219540688451 1104 5 0.9964232832384994 1104 6 0.8506337988201977 1104 7 0.6396549940915648 1104 8 0.7142023328905296 1104 9 0.7718382604719309 1104 10 0.5699653089207217 1104 11 0.7058992875701549 1104 12 0.6577823054606924 1104 13 0.6637813956039977 1104 14 0.8352971453497133 1104 15 0.8615043946837062 1104 16 0.6764565456159488 1104 17 0.703782902562267 1104 18 0.5973804345400353 1104 19 0.7966529033405436 1104 20 0.7997354439784203 1104 21 0.7486649139788 1104 22 0.9604328201592693 1104 23 0.9671457767919449 1104 24 0.701222158796341 1104 25 0.7486145423398014 1105 1 1.372243545813209 1105 2 3.8375899480407725 1105 3 2.376489239268267 1105 4 1.449873155350211 1105 5 1.0967261973221127 1105 6 0.8674176404492329 1105 7 0.8647472030737262 1105 8 0.8361644491041602 1105 9 0.8949293829967999 1105 10 0.6548200127445369 1105 11 0.6472031995158218 1105 12 0.7538929748909098 1105 13 0.614327771709398 1105 14 0.7291967809435462 1105 15 0.848605628721991 1105 16 0.8327152157082978 1105 17 0.9536500097181957 1105 18 0.8150248156207525 1105 19 0.8078182336958335 1105 20 0.7938869931126362 1105 21 0.8570667143271251 1105 22 0.9545164657130698 1105 23 1.0678817583454858 1105 24 0.968050589471737 1105 25 0.9137738272364118 1106 1 1.0736768843457156 1106 2 3.750494606831385 1106 3 2.250200882417481 1106 4 1.1626510482500123 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0.8826757342063516 1107 18 0.6646269115591181 1107 19 0.865454508695521 1107 20 1.078036111215944 1107 21 1.0655834649846412 1107 22 1.0677348963519577 1107 23 1.1824464013338556 1107 24 1.1742806351797341 1107 25 0.9938430112989067 1108 1 1.3813954956622148 1108 2 3.9757140590649698 1108 3 2.4506178532868717 1108 4 1.4830573545856396 1108 5 1.0599178049766493 1108 6 0.9233623794369663 1108 7 0.8007511542305821 1108 8 0.7406951733495575 1108 9 0.7037298123264151 1108 10 0.5150977801753598 1108 11 0.7542563732025584 1108 12 0.8172273705115245 1108 13 0.7249833409229325 1108 14 0.7097185709390104 1108 15 0.7379597166655856 1108 16 0.7592228703849102 1108 17 0.7050574024099348 1108 18 0.7747263266899225 1108 19 0.6802226312892685 1108 20 0.8858225771154196 1108 21 0.739422674379604 1108 22 0.9055342610944663 1108 23 0.7837952357360081 1108 24 0.7320442930052948 1108 25 0.8540959072225078 1109 1 1.0180880021221625 1109 2 3.0886517082355347 1109 3 1.8399742254587963 1109 4 1.1577402746795329 1109 5 0.7116762916721129 1109 6 0.4995384845319393 1109 7 0.44185812308475647 1109 8 0.41385926589941135 1109 9 0.4743158430580152 1109 10 0.39021070598951724 1109 11 0.30615096262599195 1109 12 0.49228901123976954 1109 13 0.4159275782545322 1109 14 0.3280352366194208 1109 15 0.32324089701155856 1109 16 0.3494589790303948 1109 17 0.3481960496671661 1109 18 0.5056926560585282 1109 19 0.3927105497065355 1109 20 0.4610163755650305 1109 21 0.3657674285070627 1109 22 0.36463046008059763 1109 23 0.24116352182789313 1109 24 0.28322878123930906 1109 25 0.41833047731541484 1110 1 0.9053673894015475 1110 2 3.469899976506703 1110 3 2.072507849595471 1110 4 1.1997303456772386 1110 5 0.5597556931866308 1110 6 0.3358576361182699 1110 7 0.2730777939990858 1110 8 0.26307976550158685 1110 9 0.19552974366123266 1110 10 0.14225382826164434 1110 11 0.24597602306174338 1110 12 0.352546056992999 1110 13 0.2543916587033479 1110 14 0.30379916734518275 1110 15 0.2688154242654761 1110 16 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-1.9197209144714478 2315 18 -2.0222788598585097 2315 19 -1.917915834682141 2315 20 -1.9440795476963615 2315 21 -2.078011517801535 2315 22 -2.036374537448097 2315 23 -2.101177948507292 2315 24 -2.020947267234888 2315 25 -1.964738792459606 2316 1 -0.4018666464039171 2316 2 1.9314067237277364 2316 3 0.5475645389378769 2316 4 -0.27985990534158844 2316 5 -0.46055891284225936 2316 6 -0.6813204239169366 2316 7 -1.1956312747099886 2316 8 -1.0534513224580735 2316 9 -1.3784407303092436 2316 10 -1.2461847173197569 2316 11 -1.2283940513083351 2316 12 -1.2945798329900313 2316 13 -1.3605727419753393 2316 14 -1.3148028655425605 2316 15 -1.5206807938021711 2316 16 -1.6705614464425906 2316 17 -1.5085902271845413 2316 18 -1.7966389636122386 2316 19 -1.6769887804071928 2316 20 -1.539437139174197 2316 21 -1.7504154314053508 2316 22 -1.6445263833183361 2316 23 -1.761076356311662 2316 24 -1.7587957639477239 2316 25 -1.8159773934155794 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57543.0 3 1.0 4 485.0 5 2554.0 6 3055.0 7 2814.0 8 2502.0 9 2524.0 10 2411.0 11 2282.0 12 2289.0 13 2231.0 14 2364.0 15 2475.0 16 2710.0 17 2740.0 18 3211.0 19 3968.0 20 4933.0 21 6484.0 22 7986.0 23 9634.0 24 11259.0 25 12935.0 26 15008.0 27 17146.0 28 20146.0 29 24707.0 30 31358.0 31 41040.0 32 53449.0 33 73281.0 34 101276.0 35 147011.0 36 231941.0 37 300357.0 38 708259.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.33168612099236 18.516997509352322 13.158947989661698 24.992368379993625 2 16.628076760913306 20.864784788322776 39.72954884991172 22.777589600852195 3 19.06694378796568 24.428489502804524 29.58892572359499 26.915640985634816 4 12.575761766131679 16.69845421210633 37.947493410715786 32.7782906110462 5 13.526048480224842 37.4613135570446 35.409516643193456 13.603121319537104 6 31.872410793537153 36.666790164107226 18.13784297283039 13.322956069525233 7 27.86650170191649 31.52023435987537 22.084786385085543 18.5284775531226 8 25.20474918974373 36.90202669736361 20.470013864761956 17.42321024813071 9 26.47011092331383 15.441076327158287 20.033563473422916 38.055249276104966 10 15.614581534140868 28.729748811424106 33.81285128281662 21.842818371618407 11 34.81761602280146 22.183358215458505 24.331430950928553 18.66759481081149 12 23.710517129008036 24.116075766201604 31.559475236762857 20.613931868027503 13 29.27833835759188 21.728957210224127 25.677152990890583 23.315551441293405 14 21.470447497324365 21.754213306518736 27.3957155433009 29.379623652856 15 23.427012229899358 30.03466451398452 23.584967195774926 22.9533560603412 16 22.66040621613061 27.51484708842608 27.360962319887243 22.463784375556063 17 22.027177438165616 27.818650792201293 27.593589752286746 22.560582017346345 18 21.858055520622592 27.0640466423742 29.450330286077474 21.627567550925736 19 23.82621709400282 26.361533901630587 27.359202407888255 22.453046596478337 20 23.82349119611098 26.772505712626327 27.902559475758583 21.50144361550411 21 23.603752721944467 26.262061220986144 27.021153024287077 23.113033032782308 22 23.55346478079479 27.205032470497375 27.225853247441105 22.015649501266726 23 23.075959711076948 26.88088551304088 27.48813113207449 22.55502364380768 24 23.574073455620216 26.272876608250606 27.893650906297747 22.25939902983143 25 23.240513700649508 26.70127725923348 27.84375034244449 22.214458697672526 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 313.0 1 313.0 2 464.5 3 616.0 4 616.0 5 616.0 6 1537.0 7 2458.0 8 2458.0 9 2458.0 10 3427.0 11 4396.0 12 4396.0 13 4396.0 14 6506.0 15 8616.0 16 8616.0 17 8616.0 18 14894.5 19 21173.0 20 21173.0 21 21173.0 22 32850.0 23 44527.0 24 44527.0 25 44527.0 26 64734.0 27 84941.0 28 84941.0 29 84941.0 30 111309.0 31 137677.0 32 137677.0 33 137677.0 34 153853.5 35 170030.0 36 170030.0 37 170030.0 38 183386.5 39 196743.0 40 196743.0 41 196743.0 42 213233.0 43 229723.0 44 229723.0 45 229723.0 46 239707.5 47 249692.0 48 249692.0 49 249692.0 50 244594.0 51 239496.0 52 239496.0 53 239496.0 54 218643.5 55 197791.0 56 197791.0 57 197791.0 58 171596.0 59 145401.0 60 145401.0 61 145401.0 62 120149.0 63 94897.0 64 94897.0 65 94897.0 66 73066.0 67 51235.0 68 51235.0 69 51235.0 70 37488.5 71 23742.0 72 23742.0 73 23742.0 74 16202.0 75 8662.0 76 8662.0 77 8662.0 78 5825.0 79 2988.0 80 2988.0 81 2988.0 82 1934.0 83 880.0 84 880.0 85 880.0 86 576.0 87 272.0 88 272.0 89 272.0 90 173.0 91 74.0 92 74.0 93 74.0 94 48.0 95 22.0 96 22.0 97 22.0 98 13.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.330481238216648 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 5.218201713761807E-5 20 0.0 21 0.0 22 7.30548239926653E-4 23 5.740021885137988E-4 24 5.218201713761807E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1916369.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.76856525414381 #Duplication Level Percentage of deduplicated Percentage of total 1 77.81822018830414 51.17992692415812 2 14.161408629767728 18.627510551149477 3 4.250864442462805 8.387197664119036 4 1.6607426710009674 4.368986509122728 5 0.7760920233495237 2.552122944044182 6 0.41244277209034236 1.6275461621894187 7 0.23779071297118973 1.0947407816012549 8 0.14969686309545155 0.787627832706707 9 0.09987301407008947 0.5911654358697032 >10 0.3862174254772973 4.25556738607765 >50 0.023979672958435613 1.0726120485070758 >100 0.018813704862855305 2.423325673529486 >500 0.0024111747432362346 1.036158475474839 >1k 0.0013663323545005204 1.6828456905018236 >5k 8.037249144120709E-5 0.31266592094855783 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5915 0.3086566313690109 No Hit TATCAACGCAGAGTACTTTTTTTTT 3577 0.18665507530125983 No Hit GGTATCAACGCAGAGTACTTTTTTT 3480 0.18159341963891087 No Hit GTCCTACAGTGGACATTTCTAAATT 3136 0.16364280574357026 No Hit CTGTAGGACGTGGAATATGGCAAGA 2627 0.13708215902052268 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2533 0.13217704940958658 No Hit CTTTAGGACGTGAAATATGGCGAGG 2284 0.11918372714231967 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCCTA 130 0.0 14.548712 16 GGTATCA 1095 0.0 13.089802 1 TCCAACG 230 0.0 12.745936 18 TAGGACC 470 0.0 12.273541 4 ATAAACG 55 0.0031757816 12.035752 3 ACGAAAT 80 3.0044861E-5 11.820828 12 GATCTAC 95 8.752639E-6 11.445855 1 TGTCGAG 75 2.164258E-4 11.347995 18 TAGACGA 60 0.006079895 11.032773 5 GTATTAA 185 2.7284841E-11 10.686548 1 AGTGCGG 80 3.9342564E-4 10.638746 10 CGAAATC 90 9.9682184E-5 10.507402 13 GTAAGAC 145 1.5501428E-8 10.4349375 3 GTCCTAC 1455 0.0 10.394596 1 GTATCAA 2180 0.0 10.247812 1 GATATAC 630 0.0 10.198868 1 AGGACCT 995 0.0 10.169476 5 TAATACA 270 0.0 10.157156 4 TTAGGAC 1075 0.0 10.116429 3 CCAACGT 170 1.6880222E-9 10.012937 19 >>END_MODULE