FastQCFastQC Report
Thu 26 May 2016
SRR1511761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511761_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916369
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74280.387608023298227No Hit
TATCAACGCAGAGTACTTTTTTTTT50150.26169281594515464No Hit
GGTATCAACGCAGAGTACTTTTTTT46750.24395093011836447No Hit
GTCCTACAGTGGACATTTCTAAATT32040.1671911829089283No Hit
CTGTAGGACGTGGAATATGGCAAGA27940.14579655588250487No Hit
GTCCTAAAGTGTGTATTTCTCATTT27320.14256127081997255No Hit
CTTTAGGACGTGAAATATGGCGAGG25070.1308203169640085No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23130.12069700563931059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14650.013.4760761
ACACCGT500.001503219213.2959466
TCCAACG2500.012.53617718
CCAACGA1551.8189894E-1212.25432719
TAGGACC4400.012.0919564
TAGCGTT550.003075909812.08722311
TTAGACC802.8646391E-511.8760273
ACCGTCC658.0454675E-411.6887448
GATATAC7900.010.9861381
GTATAAG1407.856215E-910.8999041
CGTTTCC1502.1445885E-910.76338514
AATCCCG3200.010.68424119
TTAGGAC9850.010.4171463
GGCGAGG7450.010.3257119
AAGGCGT1201.5298938E-610.2885286
TATACAC10150.010.1092493
AAAGCCG856.5891905E-410.0596945
GCGACTA1054.1193423E-59.94934716
TAAGCGA1054.1193423E-59.94934713
ACTGTTC8700.09.9337528
Produced by FastQC (version 0.11.5)