##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511761_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1916369 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.67741651007713 31.0 31.0 34.0 27.0 34.0 2 30.864628367501247 31.0 31.0 34.0 27.0 34.0 3 30.96533496419531 31.0 31.0 34.0 27.0 34.0 4 34.54428818249512 37.0 35.0 37.0 31.0 37.0 5 34.13658068983583 37.0 35.0 37.0 30.0 37.0 6 34.08858158319196 37.0 35.0 37.0 30.0 37.0 7 33.89855607140378 37.0 35.0 37.0 29.0 37.0 8 33.79662163184648 37.0 35.0 37.0 29.0 37.0 9 35.26370704180667 39.0 35.0 39.0 29.0 39.0 10 35.04030225911607 38.0 35.0 39.0 28.0 39.0 11 35.04037479211989 38.0 35.0 39.0 28.0 39.0 12 34.84596442543164 38.0 35.0 39.0 27.0 39.0 13 34.82646087470628 38.0 35.0 39.0 27.0 39.0 14 35.78503461494107 39.0 36.0 41.0 27.0 41.0 15 35.69162045514199 39.0 36.0 41.0 27.0 41.0 16 35.52785345619763 39.0 36.0 41.0 27.0 41.0 17 35.4878695073861 39.0 35.0 41.0 27.0 41.0 18 35.405332689059364 39.0 35.0 40.0 27.0 41.0 19 35.417003719012364 39.0 35.0 40.0 27.0 41.0 20 35.52356200710823 39.0 36.0 41.0 27.0 41.0 21 35.46235250100581 39.0 36.0 41.0 27.0 41.0 22 35.34636283513248 39.0 35.0 41.0 27.0 41.0 23 35.22254638850868 39.0 35.0 41.0 26.0 41.0 24 35.040713975231284 39.0 35.0 41.0 25.0 41.0 25 34.47751033334394 38.0 34.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31687189413685246 1101 2 0.40753239249797346 1101 3 0.3992870300759037 1101 4 0.43028042585596893 1101 5 0.623311304948281 1101 6 0.4065137351258983 1101 7 0.6187936111675398 1101 8 0.30432025594234346 1101 9 0.3518224883392804 1101 10 0.5788904233905825 1101 11 0.4256290365955806 1101 12 0.43792133398017086 1101 13 0.510294223913867 1101 14 0.2180082659829239 1101 15 0.19990964417506518 1101 16 0.12748091364324665 1101 17 -0.004192803165103953 1101 18 0.26681768485358504 1101 19 0.22933936495866902 1101 20 0.11288620415390227 1101 21 0.15175984702201362 1101 22 0.16573170830181283 1101 23 -0.045245143973446034 1101 24 -0.5534185687260873 1101 25 -0.22489561448135476 1102 1 0.7990179618018409 1102 2 0.7812549535311213 1102 3 0.6542828035388126 1102 4 0.7564214170409969 1102 5 0.90031155956693 1102 6 0.8746471685408039 1102 7 1.0787855388624692 1102 8 0.9516843569646909 1102 9 1.1249844248051417 1102 10 1.1865618282597268 1102 11 1.218006568834305 1102 12 1.3289260114803199 1102 13 1.2338075109346462 1102 14 1.49119155862914 1102 15 1.3124351929544034 1102 16 1.4378487928884525 1102 17 1.4662146495789656 1102 18 1.436111272072722 1102 19 1.464415947559246 1102 20 1.4607214324481603 1102 21 1.3996911946830792 1102 22 1.387464485866765 1102 23 1.379882570951679 1102 24 1.422902559357027 1102 25 1.480581798791853 1103 1 0.37554866909251317 1103 2 0.29514381565424586 1103 3 0.34687232699043946 1103 4 0.5255339392431964 1103 5 0.6308833084098424 1103 6 0.5147283651631724 1103 7 0.391603235101023 1103 8 0.5292168532790456 1103 9 0.5674475603180653 1103 10 0.7469881105271909 1103 11 0.46192147939918016 1103 12 0.48124515788798305 1103 13 0.7257633281523113 1103 14 0.8315011207519447 1103 15 0.8120218224916016 1103 16 0.8845789265352622 1103 17 1.081422956815679 1103 18 0.7750734456240806 1103 19 1.0100023265688876 1103 20 0.9045277810733126 1103 21 0.976926591014454 1103 22 0.9316919134948165 1103 23 0.9226238562864992 1103 24 0.8326924052825859 1103 25 0.8888055263206596 1104 1 0.141938400915965 1104 2 0.3619208861893668 1104 3 0.32446404238283577 1104 4 0.36594052153417067 1104 5 0.31303365504098224 1104 6 0.4273908870589125 1104 7 0.40516379069769215 1104 8 0.608243202179942 1104 9 0.517385265397877 1104 10 0.6689794509165026 1104 11 0.7743903319390881 1104 12 0.8546384423722273 1104 13 0.6447151698615343 1104 14 0.8152982736890593 1104 15 0.7355489485370441 1104 16 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-1.2478254900577355 2315 7 -1.2378591272125163 2315 8 -1.2846579790584727 2315 9 -1.3568548621507404 2315 10 -1.5489331185912079 2315 11 -1.227702753417951 2315 12 -1.3038293248986719 2315 13 -1.3198535681611503 2315 14 -1.5948620260187738 2315 15 -1.6717956997617804 2315 16 -1.4749930768789525 2315 17 -1.6625787909386602 2315 18 -1.5347922211645226 2315 19 -1.3444513985865783 2315 20 -1.3169594359327448 2315 21 -1.4479382628651507 2315 22 -1.6923513942318849 2315 23 -1.6774990961989715 2315 24 -1.5441701724518424 2315 25 -1.6231813248763487 2316 1 -0.9191897912633813 2316 2 -1.2272321403778825 2316 3 -1.1205668520710148 2316 4 -1.0992863843664722 2316 5 -1.3645431107453874 2316 6 -1.275594091503109 2316 7 -1.7600705417543523 2316 8 -1.9374229099708877 2316 9 -1.7544940977010768 2316 10 -1.6422274627077513 2316 11 -1.8548316625355596 2316 12 -1.9563850354322838 2316 13 -1.9971237927977654 2316 14 -2.051246102431115 2316 15 -2.1041932511084624 2316 16 -2.0522480076282292 2316 17 -1.9457110606331085 2316 18 -2.0477675551830217 2316 19 -2.1029804936778547 2316 20 -2.048921621286503 2316 21 -2.1464985982702984 2316 22 -2.108886125630441 2316 23 -1.972917863226165 2316 24 -1.9639331375899332 2316 25 -2.3457230873847124 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35506.0 3 3.0 4 656.0 5 5532.0 6 6876.0 7 5482.0 8 4486.0 9 4038.0 10 3865.0 11 3563.0 12 3449.0 13 3288.0 14 3326.0 15 3198.0 16 3315.0 17 3489.0 18 3686.0 19 4198.0 20 5101.0 21 6552.0 22 8571.0 23 10646.0 24 12819.0 25 15233.0 26 17521.0 27 19964.0 28 23405.0 29 28097.0 30 36209.0 31 45738.0 32 59170.0 33 76502.0 34 100498.0 35 134631.0 36 200422.0 37 356996.0 38 660338.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.14079715961846 19.439961519034036 13.982098805539078 24.437142515808425 2 15.34313068099098 20.785610704410267 40.31869645146629 23.552562163132464 3 19.269253468408223 25.037036186663425 30.037847617029918 25.655862727898437 4 13.240351936396383 17.559092220757066 37.53509892927719 31.66545691356936 5 13.534024936552 37.70737395745356 35.3531425421586 13.40545856383584 6 31.883577745204605 37.21976299971456 17.74694748245249 13.14971177262834 7 27.758693654510168 31.7337631740025 22.1313327443723 18.376210427115026 8 25.347258278546565 37.08962104897334 20.212130336067847 17.35099033641225 9 26.56513437652143 15.286930648533763 19.908744088429735 38.239190886515075 10 15.515383519562256 29.080098874486072 33.754042149502524 21.65047545644915 11 34.916396581242964 22.237940605384455 24.23270257450418 18.6129602388684 12 23.64643761196304 24.453641235064854 31.46319941514395 20.436721737828154 13 29.46337579036188 21.725095740955943 25.693433780237523 23.118094688444657 14 21.484275731865836 21.773833744962477 27.4079261353111 29.333964387860583 15 23.434943896504272 30.262856474927325 23.573904608141753 22.72829502042665 16 22.777450480570288 27.569116386249203 27.275644721867238 22.377788411313272 17 21.960332274212323 27.955002402981886 27.623020409952364 22.461644912853423 18 21.96810739476583 27.049174767489976 29.52855112976676 21.45416670797743 19 23.87885631629399 26.40175248086355 27.43406932589705 22.28532187694541 20 23.869359189174947 26.776054089791685 28.0135506262103 21.341036094823075 21 23.662196581138602 26.230699828686436 27.149051148291374 22.958052441883584 22 23.616693862194598 27.128439251522018 27.427494391737707 21.827372494545674 23 23.163701771422936 26.91308406679507 27.508585246369567 22.41462891541243 24 23.596238511476653 26.431809322734818 27.89196652627965 22.079985639508884 25 23.273752712855085 26.774490317214227 27.91244137910881 22.039315590821875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 284.0 1 284.0 2 493.5 3 703.0 4 703.0 5 703.0 6 1730.0 7 2757.0 8 2757.0 9 2757.0 10 3671.0 11 4585.0 12 4585.0 13 4585.0 14 6644.0 15 8703.0 16 8703.0 17 8703.0 18 14985.5 19 21268.0 20 21268.0 21 21268.0 22 33962.5 23 46657.0 24 46657.0 25 46657.0 26 67453.5 27 88250.0 28 88250.0 29 88250.0 30 114048.5 31 139847.0 32 139847.0 33 139847.0 34 155687.5 35 171528.0 36 171528.0 37 171528.0 38 184819.0 39 198110.0 40 198110.0 41 198110.0 42 213728.5 43 229347.0 44 229347.0 45 229347.0 46 238325.0 47 247303.0 48 247303.0 49 247303.0 50 242929.5 51 238556.0 52 238556.0 53 238556.0 54 217724.0 55 196892.0 56 196892.0 57 196892.0 58 169980.0 59 143068.0 60 143068.0 61 143068.0 62 118048.5 63 93029.0 64 93029.0 65 93029.0 66 71625.5 67 50222.0 68 50222.0 69 50222.0 70 36530.0 71 22838.0 72 22838.0 73 22838.0 74 15715.0 75 8592.0 76 8592.0 77 8592.0 78 5705.0 79 2818.0 80 2818.0 81 2818.0 82 1783.5 83 749.0 84 749.0 85 749.0 86 477.5 87 206.0 88 206.0 89 206.0 90 129.5 91 53.0 92 53.0 93 53.0 94 28.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4118726612672194 2 0.0 3 0.0 4 0.0 5 0.03271812474528653 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0015654605141285421 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1916369.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.93985821941585 #Duplication Level Percentage of deduplicated Percentage of total 1 76.92603107951302 49.1863952060644 2 14.443216667296166 18.469944518784416 3 4.497874193296892 8.627803146245181 4 1.818592495765228 4.651221853524891 5 0.8214286658200383 2.626101621494858 6 0.46400423555988374 1.7801019020944437 7 0.275726555573791 1.2340941809501258 8 0.16149170251336795 0.8260605249853463 9 0.11373701338349546 0.6545095659036452 >10 0.42555909332011493 4.543715651034008 >50 0.02772291618644592 1.212098998918994 >100 0.019970800688911584 2.4798434837613037 >500 0.003096387388507103 1.3175740074330502 >1k 0.001385225936963696 1.742246621399067 >5k 1.629677572898466E-4 0.6482887174063574 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7428 0.387608023298227 No Hit TATCAACGCAGAGTACTTTTTTTTT 5015 0.26169281594515464 No Hit GGTATCAACGCAGAGTACTTTTTTT 4675 0.24395093011836447 No Hit GTCCTACAGTGGACATTTCTAAATT 3204 0.1671911829089283 No Hit CTGTAGGACGTGGAATATGGCAAGA 2794 0.14579655588250487 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2732 0.14256127081997255 No Hit CTTTAGGACGTGAAATATGGCGAGG 2507 0.1308203169640085 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2313 0.12069700563931059 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1465 0.0 13.476076 1 ACACCGT 50 0.0015032192 13.295946 6 TCCAACG 250 0.0 12.536177 18 CCAACGA 155 1.8189894E-12 12.254327 19 TAGGACC 440 0.0 12.091956 4 TAGCGTT 55 0.0030759098 12.087223 11 TTAGACC 80 2.8646391E-5 11.876027 3 ACCGTCC 65 8.0454675E-4 11.688744 8 GATATAC 790 0.0 10.986138 1 GTATAAG 140 7.856215E-9 10.899904 1 CGTTTCC 150 2.1445885E-9 10.763385 14 AATCCCG 320 0.0 10.684241 19 TTAGGAC 985 0.0 10.417146 3 GGCGAGG 745 0.0 10.32571 19 AAGGCGT 120 1.5298938E-6 10.288528 6 TATACAC 1015 0.0 10.109249 3 AAAGCCG 85 6.5891905E-4 10.059694 5 GCGACTA 105 4.1193423E-5 9.949347 16 TAAGCGA 105 4.1193423E-5 9.949347 13 ACTGTTC 870 0.0 9.933752 8 >>END_MODULE