FastQCFastQC Report
Thu 26 May 2016
SRR1511760_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511760_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979041
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49240.5029411434250455No Hit
GGTATCAACGCAGAGTACTTTTTTT29710.3034602228098721No Hit
TATCAACGCAGAGTACTTTTTTTTT28640.2925311605949087No Hit
GTACTGGTTCACTATCGGTCAGTCA16620.1697579570212075No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13890.14187352725779614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6700.015.047051
GTCTTAG1003.619789E-1014.8257711
GTGTTAT602.9265552E-413.1784641
GTCTAAA450.0088535413.1784631
GGTCTAG550.002278750512.5794421
TAAACCA752.1619217E-411.3481514
CTAGGGT1002.0355783E-611.3481514
ACTAGAC600.006075674711.0329253
TGGACTG1658.731149E-1110.889645
GTATCAA15250.010.8884351
GTCTTAA1001.5461863E-510.8722321
TTAGTAC700.001548309710.8077633
GCCCTAA750.001903882310.542771
GACTGTC856.870196E-410.0130757
TTACCCT856.870196E-410.0130754
GTATTAG800.0032529919.8838471
GACTTAA800.0032529919.8838471
TGTTATA1104.4227218E-59.8838472
GTCTTAC800.0032529919.8838471
GCGTACA1501.4840589E-79.8838472
Produced by FastQC (version 0.11.5)