FastQCFastQC Report
Thu 26 May 2016
SRR1511760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511760_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979041
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68310.6977235886954684No Hit
TATCAACGCAGAGTACTTTTTTTTT46140.4712775052321609No Hit
GGTATCAACGCAGAGTACTTTTTTT43930.44870439542368495No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22430.22910174344077522No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13370.13656220730286064No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12120.12379461125734265No Hit
ATCATTAACTGAATCCATAGGTTAA11410.11654261670348842No Hit
GGTATCAACGCAGAGTACATGGGGG11130.11368267518929238No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGGC402.7611092E-416.6239723
CCGAATA350.002173288716.2813814
GGTATCA11500.014.5963911
TGGACCG550.00306847112.0901615
GTATTAA1156.6856956E-811.61076451
CCCCGTA1157.086783E-811.562139515
CCGTACA1251.8349965E-811.39696717
TAGGAGT1104.9643677E-711.2265784
TACCCTC600.00587573111.0826475
GTCTTAG700.001442452210.8999011
CCCGTAC1252.243869E-710.63716916
GTGGTAT3050.010.6318711
CTTACAC1803.45608E-1110.5549033
GTATTAT1002.2941447E-510.4911561
GGAGTGC1002.4029321E-510.4472196
CGCCCTA1002.4029321E-510.44721916
AGGAGTG1659.276846E-1010.3629955
ATTGGAC750.002647432310.1327073
TGTAGCA1502.440538E-810.1327072
GTCAGAT856.592046E-410.0582013
Produced by FastQC (version 0.11.5)