##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511760_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 979041 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.972707986693102 31.0 30.0 33.0 25.0 34.0 2 29.15639079466539 31.0 30.0 34.0 25.0 34.0 3 29.165686625994212 31.0 29.0 34.0 25.0 34.0 4 32.745122012254846 35.0 32.0 37.0 26.0 37.0 5 32.166049225721906 35.0 32.0 37.0 24.0 37.0 6 32.14296745488698 35.0 32.0 37.0 23.0 37.0 7 31.885453213910345 35.0 32.0 37.0 21.0 37.0 8 31.7264496583902 35.0 32.0 37.0 19.0 37.0 9 32.88910474637937 37.0 32.0 39.0 18.0 39.0 10 32.57450198714865 37.0 32.0 39.0 17.0 39.0 11 32.620614458434325 37.0 32.0 39.0 17.0 39.0 12 32.31236383358817 37.0 32.0 39.0 16.0 39.0 13 32.26512781385049 37.0 32.0 39.0 15.0 39.0 14 32.87030573796194 38.0 32.0 40.0 11.0 41.0 15 32.71761039629597 38.0 32.0 40.0 10.0 41.0 16 32.50298097832471 37.0 31.0 40.0 10.0 41.0 17 32.443256206839145 37.0 31.0 40.0 10.0 41.0 18 32.31095224816938 37.0 31.0 40.0 10.0 41.0 19 32.31948713077389 37.0 31.0 40.0 8.0 41.0 20 32.395011036310024 38.0 32.0 40.0 2.0 41.0 21 32.314482233124046 38.0 32.0 40.0 2.0 41.0 22 32.11631279997467 37.0 31.0 40.0 2.0 41.0 23 31.912245758859946 37.0 31.0 40.0 2.0 41.0 24 31.60425661438081 37.0 31.0 40.0 2.0 41.0 25 30.89629239224915 37.0 30.0 39.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4351226920150246 1101 2 0.46553999388249423 1101 3 0.462797088118716 1101 4 0.5518468549269784 1101 5 0.6695092859501983 1101 6 0.2920293494335695 1101 7 0.32398771940105675 1101 8 0.1114598746886486 1101 9 0.22117679700209436 1101 10 0.2992970589293975 1101 11 0.06793540928322983 1101 12 0.22067840670462147 1101 13 0.159546793716963 1101 14 0.3531606027198322 1101 15 0.03853167386153444 1101 16 -0.18720829862018462 1101 17 -0.1968910175911205 1101 18 -0.08643256094806162 1101 19 -0.29343919601495116 1101 20 -0.7728422389693321 1101 21 -0.36950129934115594 1101 22 -0.21642578918043043 1101 23 0.07146605285786123 1101 24 -0.9098748005368051 1101 25 -0.5238102267445583 1102 1 0.720580979920225 1102 2 0.6160867544668491 1102 3 0.6227543936306184 1102 4 0.8985047128322421 1102 5 1.046160212684093 1102 6 0.7018047833000765 1102 7 1.0053590984368377 1102 8 0.8391545823669695 1102 9 0.9329804099945065 1102 10 1.2772347394883212 1102 11 1.0466807717949465 1102 12 1.4266420782014677 1102 13 1.2656825033791677 1102 14 1.559411640963475 1102 15 1.1710134723763872 1102 16 1.2720715469464423 1102 17 1.6624902178376644 1102 18 1.731763577835757 1102 19 2.0141997949745303 1102 20 1.8026855706850426 1102 21 1.56530177328721 1102 22 1.5224332598170278 1102 23 1.7984838734149875 1102 24 1.6162545698722468 1102 25 1.8002783624088288 1103 1 0.16849954153176583 1103 2 0.3992889730837774 1103 3 0.5439816125259895 1103 4 0.6212592727657125 1103 5 0.9450898859429984 1103 6 0.9902528280882166 1103 7 0.7426241407412881 1103 8 0.8025600257005401 1103 9 0.30910948776119085 1103 10 0.840355430615709 1103 11 0.6313509307289991 1103 12 0.9607399483143695 1103 13 0.8169174814937179 1103 14 1.253564753746879 1103 15 1.2032606387763707 1103 16 1.18163209298757 1103 17 1.2074324320737873 1103 18 1.0622023635365139 1103 19 1.1283624118732902 1103 20 1.0178888139644116 1103 21 1.2436969885591367 1103 22 1.3761812596833778 1103 23 1.3534035525025914 1103 24 1.070406494641233 1103 25 1.2864045815158143 1104 1 0.5327870269527004 1104 2 0.5336781216662807 1104 3 0.5075757313075009 1104 4 0.5896784886644681 1104 5 0.8846209680541435 1104 6 0.9985980236543455 1104 7 1.0232456911274603 1104 8 0.943766208143213 1104 9 1.2369506838415987 1104 10 1.1283698306010166 1104 11 1.1092991164961603 1104 12 1.289301143660083 1104 13 1.0283924697845492 1104 14 1.3998880738251245 1104 15 1.4621159868831626 1104 16 1.4192465444013962 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1.626225949435721 1107 17 1.4811345707095853 1107 18 1.35936007939155 1107 19 1.5829913179751145 1107 20 1.2484218418144692 1107 21 1.1935710525740646 1107 22 1.2893157760057434 1107 23 1.4507839322830378 1107 24 1.2497799251829846 1107 25 1.4013211678919681 1108 1 0.08667718713576633 1108 2 0.24576466212810644 1108 3 -0.05834896184831351 1108 4 0.2764165789494726 1108 5 0.16238256572420795 1108 6 0.20842743909017258 1108 7 0.06229291392754632 1108 8 0.04208116549706986 1108 9 0.13821966657511808 1108 10 0.3030525849176371 1108 11 -0.04410217328957344 1108 12 0.18854183431954397 1108 13 0.019571999636845305 1108 14 0.0993367944408945 1108 15 0.32179978134956144 1108 16 0.45725361741990866 1108 17 -0.18586147672124298 1108 18 -0.03886126448196592 1108 19 -0.1620679705624326 1108 20 -0.041103849885132604 1108 21 0.03645642806813498 1108 22 -0.02590161529860069 1108 23 0.07528986113679537 1108 24 0.07191431253476566 1108 25 0.3311630651251676 1109 1 -0.10661981911675866 1109 2 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-1.6745276179507194 2315 15 -1.5680601197327384 2315 16 -1.0968034568511413 2315 17 -1.5587863779070155 2315 18 -1.2683751605562996 2315 19 -1.1144177832810165 2315 20 -1.2345003691409744 2315 21 -1.774637667911005 2315 22 -1.5138007523554897 2315 23 -1.2516310667857269 2315 24 -1.2260069671871072 2315 25 -1.5567815989064542 2316 1 -1.4696857588227772 2316 2 -1.6469334409838936 2316 3 -1.7880553670815864 2316 4 -2.038041366750168 2316 5 -2.0964157842076148 2316 6 -2.114846116668673 2316 7 -2.0210001457752362 2316 8 -2.829033148326566 2316 9 -2.4222770798908755 2316 10 -2.443434979160582 2316 11 -2.3775419440750802 2316 12 -2.6337478941786543 2316 13 -2.6914201124849413 2316 14 -2.636315724674695 2316 15 -2.593858606209338 2316 16 -2.494021042987086 2316 17 -2.1415374696100713 2316 18 -2.4043487767798055 2316 19 -2.4518180439179744 2316 20 -2.4722229965444384 2316 21 -2.289332791256303 2316 22 -2.4450120283334513 2316 23 -2.43359910446377 2316 24 -2.6605882333340247 2316 25 -2.4358209002164983 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40454.0 3 0.0 4 635.0 5 5063.0 6 6195.0 7 5118.0 8 4110.0 9 3676.0 10 3468.0 11 3318.0 12 3045.0 13 2982.0 14 2820.0 15 3000.0 16 2999.0 17 2955.0 18 3332.0 19 3769.0 20 4586.0 21 5884.0 22 7044.0 23 8989.0 24 10556.0 25 12325.0 26 13998.0 27 15843.0 28 18569.0 29 21740.0 30 27372.0 31 34423.0 32 43536.0 33 55686.0 34 71843.0 35 95644.0 36 130188.0 37 171304.0 38 132572.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.37725655824253 22.042255686885564 15.614204143558622 20.966283611313287 2 17.894041209714402 23.05041361904149 37.24552904321678 21.810016128027325 3 20.707304392768027 26.466920180053748 30.24990781795655 22.575867609221678 4 15.895452795133197 19.4910121230878 36.01534562903903 28.598189452739973 5 14.140574074490322 38.352994305027394 34.39696022918842 13.109471391293864 6 31.51052918110682 38.35140714229537 17.164960405131144 12.973103271466671 7 27.926716041514094 33.00893425300881 21.482961387725336 17.58138831775176 8 26.33740568576801 35.91187703068615 19.8593317338089 17.891385549736935 9 26.747194448444954 16.092073774234176 19.969848045178903 37.19088373214197 10 16.189516067253567 29.75697646983119 32.72498291695649 21.32852454595875 11 35.18259194456616 23.782558646675675 22.6356199587147 18.39922945004346 12 23.608102214309717 26.054577898167697 30.09005751546667 20.24726237205592 13 29.185192448528714 22.224911929122477 25.827723251630935 22.762172370717877 14 22.268219614908872 22.10765432704044 27.78045046121664 27.843675596834043 15 24.451580679460818 29.132896375126272 24.170489284922695 22.245033660490215 16 23.37889833010058 28.944242375957696 25.62824233101576 22.048616962925966 17 22.25575844116845 28.44763396017123 27.069857135707288 22.22675046295303 18 22.328686949780447 27.508347454294558 28.516783260353755 21.646182335571236 19 23.44375771801181 27.552676547764598 28.468368536149153 20.535197198074442 20 23.807685275693256 26.52105478728674 28.53220651637674 21.13905342064326 21 24.22462389215569 26.40910850515964 28.18993280158849 21.176334801096175 22 23.77500022981673 26.528511063377323 28.412293254317234 21.28419545248871 23 23.797266917320112 26.58039857370631 28.1221113313947 21.500223177578874 24 23.356120938755375 26.78968500808444 28.253566500279355 21.60062755288083 25 23.479458155350315 26.75066852157144 28.277594262052286 21.49227906102596 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 154.0 1 154.0 2 311.0 3 468.0 4 468.0 5 468.0 6 1135.5 7 1803.0 8 1803.0 9 1803.0 10 2373.5 11 2944.0 12 2944.0 13 2944.0 14 4143.5 15 5343.0 16 5343.0 17 5343.0 18 9409.0 19 13475.0 20 13475.0 21 13475.0 22 20199.0 23 26923.0 24 26923.0 25 26923.0 26 37538.5 27 48154.0 28 48154.0 29 48154.0 30 58491.0 31 68828.0 32 68828.0 33 68828.0 34 77574.5 35 86321.0 36 86321.0 37 86321.0 38 94151.0 39 101981.0 40 101981.0 41 101981.0 42 109756.0 43 117531.0 44 117531.0 45 117531.0 46 122702.5 47 127874.0 48 127874.0 49 127874.0 50 125169.0 51 122464.0 52 122464.0 53 122464.0 54 112027.0 55 101590.0 56 101590.0 57 101590.0 58 85929.5 59 70269.0 60 70269.0 61 70269.0 62 57146.0 63 44023.0 64 44023.0 65 44023.0 66 33780.0 67 23537.0 68 23537.0 69 23537.0 70 16817.5 71 10098.0 72 10098.0 73 10098.0 74 6856.5 75 3615.0 76 3615.0 77 3615.0 78 2433.0 79 1251.0 80 1251.0 81 1251.0 82 780.5 83 310.0 84 310.0 85 310.0 86 186.0 87 62.0 88 62.0 89 62.0 90 41.5 91 21.0 92 21.0 93 21.0 94 11.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3627018684610757 2 0.0 3 0.0 4 0.0 5 0.02890583744705278 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0015321115254621613 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 979041.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.76703147961226 #Duplication Level Percentage of deduplicated Percentage of total 1 79.04966428205941 52.77911423573043 2 12.913583751404229 17.244033056892313 3 4.17260055908483 8.357764586407939 4 1.6535777531039217 4.416179115819043 5 0.8324667696960666 2.7790667509014204 6 0.43736069127898 1.752076502554118 7 0.24913876362234852 1.1643978971495514 8 0.16465695860790627 0.8794925070969033 9 0.10884201065764565 0.6540352156694988 >10 0.37992154005800277 4.175490131263688 >50 0.021236739923894233 0.9691981654079896 >100 0.014506911973881502 1.9394096183955833 >500 0.0012216342644421287 0.5565784412739743 >1k 0.0010689299813868625 1.6367015419987376 >5k 1.5270428305526608E-4 0.69646223343882 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6831 0.6977235886954684 No Hit TATCAACGCAGAGTACTTTTTTTTT 4614 0.4712775052321609 No Hit GGTATCAACGCAGAGTACTTTTTTT 4393 0.44870439542368495 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2243 0.22910174344077522 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1337 0.13656220730286064 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1212 0.12379461125734265 No Hit ATCATTAACTGAATCCATAGGTTAA 1141 0.11654261670348842 No Hit GGTATCAACGCAGAGTACATGGGGG 1113 0.11368267518929238 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAAGGC 40 2.7611092E-4 16.623972 3 CCGAATA 35 0.0021732887 16.28138 14 GGTATCA 1150 0.0 14.596391 1 TGGACCG 55 0.003068471 12.090161 5 GTATTAA 115 6.6856956E-8 11.6107645 1 CCCCGTA 115 7.086783E-8 11.5621395 15 CCGTACA 125 1.8349965E-8 11.396967 17 TAGGAGT 110 4.9643677E-7 11.226578 4 TACCCTC 60 0.005875731 11.082647 5 GTCTTAG 70 0.0014424522 10.899901 1 CCCGTAC 125 2.243869E-7 10.637169 16 GTGGTAT 305 0.0 10.631871 1 CTTACAC 180 3.45608E-11 10.554903 3 GTATTAT 100 2.2941447E-5 10.491156 1 GGAGTGC 100 2.4029321E-5 10.447219 6 CGCCCTA 100 2.4029321E-5 10.447219 16 AGGAGTG 165 9.276846E-10 10.362995 5 ATTGGAC 75 0.0026474323 10.132707 3 TGTAGCA 150 2.440538E-8 10.132707 2 GTCAGAT 85 6.592046E-4 10.058201 3 >>END_MODULE