FastQCFastQC Report
Thu 26 May 2016
SRR1511759_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511759_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801554
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55810.3097881051581024No Hit
TATCAACGCAGAGTACTTTTTTTTT34680.19250047459027042No Hit
GGTATCAACGCAGAGTACTTTTTTT33910.18822638677497316No Hit
GTCCTACAGTGGACATTTCTAAATT21280.11812024507730548No Hit
CTGTAGGACGTGGAATATGGCAAGA19050.10574204270313296No Hit
GTCCTAAAGTGTGTATTTCTCATTT19010.10552001216727336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG707.541315E-512.7065451
TCCAACG2300.012.33508418
GTCAACG600.00437713311.5300132
TTACACG752.16362E-411.3482774
ACACGCC600.00607852611.0330496
CGCCCTA951.4307207E-510.95009216
TACTAGG1001.5499738E-510.8711542
GGTATCA13250.010.8152141
TAGGACC3950.010.7736824
GTCTAAC1209.1697075E-710.706441
GGCGAGG6100.010.69714719
AATCCCG3200.010.34348219
CCCGTTT1651.0059011E-910.31661612
TATACAG4500.010.0873585
TCTGCGC750.002749690610.08735758
CCGGTTT1502.6231646E-810.087357512
GTAGGAC12800.010.0479553
TGGCGAG12250.010.03589218
TGTAGGA13300.010.0314832
GTATTAG1603.6023266E-89.8828671
Produced by FastQC (version 0.11.5)