FastQCFastQC Report
Thu 26 May 2016
SRR1511759_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511759_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1801554
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68930.38261412092005015No Hit
TATCAACGCAGAGTACTTTTTTTTT43920.24378952837383724No Hit
GGTATCAACGCAGAGTACTTTTTTT43410.2409586390416274No Hit
GTCCTACAGTGGACATTTCTAAATT23470.1302764169156184No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21130.117287630567832No Hit
GTCCTAAAGTGTGTATTTCTCATTT21130.117287630567832No Hit
CTGTAGGACGTGGAATATGGCAAGA20980.11645501605835852No Hit
CTTTAGGACGTGAAATATGGCGAGG19650.10907250074102691No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18640.1034662297105721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC5900.012.2380574
AGTCGTC1001.9375293E-611.3962878
TGGCGAG11800.011.02606918
GTCTTAT2000.010.9722971
GTCCTAC13200.010.6253471
GCCGGTT1701.4006218E-1010.61418911
CCGGTTT1701.4006218E-1010.61418912
GCGACTA909.5475654E-510.55211716
AAGTCGT909.5475654E-510.5521177
GGACCTG8800.010.468186
GTCCTAT2650.010.4412351
GGCGAGG5500.010.3602619
AGGACCT10100.010.3471855
ATGGCGA13050.010.33379717
TATGGCG12650.010.28518616
GTAGGAC15000.010.2606453
GGCGAGA7250.010.08636919
GGTATCA14600.010.063931
TAATACC1701.5570549E-910.0594564
CAAGTCG951.6495664E-49.9967436
Produced by FastQC (version 0.11.5)