FastQCFastQC Report
Thu 26 May 2016
SRR1511758_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511758_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474411
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57850.23379301175107933No Hit
GTCCTACAGTGGACATTTCTAAATT52450.2119696364104427No Hit
CTGTAGGACGTGGAATATGGCAAGA48470.19588500051123278No Hit
GTCCTAAAGTGTGTATTTCTCATTT46120.18638779087225202No Hit
CTTTAGGACGTGAAATATGGCGAGG40820.16496855211199757No Hit
GGTATCAACGCAGAGTACTTTTTTT35630.14399386359016347No Hit
TATCAACGCAGAGTACTTTTTTTTT35600.14387262261604883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG707.616998E-613.5096964
CGGTAGG1101.5243131E-913.4767581
GGTATCA14800.012.8879091
AGAACCG854.1522617E-612.2381955
TAGGACC7950.012.1332374
CCCGTTT2600.012.00284612
TCCAACG4500.011.97908218
CCAACGT1603.6379788E-1211.82146319
CCAACGA2950.011.54095419
ATCCCGT3150.011.40818910
TCCCGTT2850.011.28178211
GACTGCG1451.3151293E-911.0872687
CGTTTCC3200.010.93441114
AATGTCC7450.010.5357498
CCGTTTC3000.010.40246713
CGCGCCT1002.5237468E-510.40246612
AGGACCT18300.010.3869645
TTAGGAC16350.010.3533033
GGCGAGG11800.010.33877119
GGACCTG17950.010.2207076
Produced by FastQC (version 0.11.5)