##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511758_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2474411 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.113237049140178 31.0 29.0 34.0 22.0 34.0 2 26.480718845818256 31.0 25.0 34.0 10.0 34.0 3 28.133948644748184 31.0 27.0 34.0 16.0 34.0 4 32.58754022674487 35.0 32.0 37.0 25.0 37.0 5 33.03678734050245 35.0 35.0 37.0 27.0 37.0 6 33.279505304494684 37.0 35.0 37.0 27.0 37.0 7 33.317013220519954 37.0 35.0 37.0 27.0 37.0 8 33.33513551305745 37.0 35.0 37.0 27.0 37.0 9 34.90118698955024 39.0 35.0 39.0 28.0 39.0 10 34.76704557165321 39.0 35.0 39.0 27.0 39.0 11 34.77467809511031 39.0 35.0 39.0 27.0 39.0 12 34.67866453875286 39.0 35.0 39.0 27.0 39.0 13 34.67523139850251 39.0 35.0 39.0 27.0 39.0 14 35.791169696545964 40.0 36.0 41.0 27.0 41.0 15 35.72996604040315 40.0 36.0 41.0 27.0 41.0 16 35.668361884909174 40.0 36.0 41.0 27.0 41.0 17 35.6388122264248 40.0 36.0 41.0 27.0 41.0 18 35.57734871046079 40.0 36.0 41.0 27.0 41.0 19 35.60105536226601 40.0 36.0 41.0 27.0 41.0 20 35.52986508708537 40.0 36.0 41.0 26.0 41.0 21 35.435363405675126 40.0 36.0 41.0 26.0 41.0 22 35.32564355719401 40.0 36.0 41.0 25.0 41.0 23 35.21561818145813 40.0 36.0 41.0 25.0 41.0 24 35.10403768816094 40.0 36.0 41.0 24.0 41.0 25 34.99159476740121 40.0 36.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9031397207232317 1101 2 3.329987467764184 1101 3 1.969276432787904 1101 4 1.0564908361530385 1101 5 0.7272408699288633 1101 6 0.4569806373079075 1101 7 0.2767709461381429 1101 8 0.20636451570407388 1101 9 0.227176500487694 1101 10 0.3729748276402205 1101 11 0.2908870000817032 1101 12 0.41559357157337473 1101 13 0.4643086133084182 1101 14 0.5018448782823555 1101 15 0.5848380242162605 1101 16 0.48971003391519474 1101 17 0.49580074024165555 1101 18 0.4927149863338016 1101 19 0.526326363477601 1101 20 0.7350029334741848 1101 21 0.6128644068474287 1101 22 0.5440389523349936 1101 23 0.7485536801313728 1101 24 0.6766915774054354 1101 25 0.48875008019350474 1102 1 1.4778654933952389 1102 2 4.038531300746971 1102 3 2.6265495293 1102 4 1.5152162796001676 1102 5 1.147288428610068 1102 6 0.9016048781001231 1102 7 0.7907404141089387 1102 8 0.7335175167051631 1102 9 0.8455529745071146 1102 10 0.8091714035225266 1102 11 0.8755988880522025 1102 12 0.8001497039683017 1102 13 0.867387000142287 1102 14 1.0395722156671852 1102 15 1.0781204499594992 1102 16 0.9363834312028487 1102 17 1.0276100814608782 1102 18 1.097803255281896 1102 19 1.0803668831877005 1102 20 1.1539089406652394 1102 21 1.0830438344076683 1102 22 0.8795694293442722 1102 23 0.8182525109357286 1102 24 0.7115699565454605 1102 25 0.523666257570568 1103 1 1.4160959119077532 1103 2 3.9638293731360434 1103 3 2.7193607930786534 1103 4 1.5865532283354185 1103 5 1.2243812282362896 1103 6 0.9982250721111754 1103 7 0.9499040873366056 1103 8 0.7127483515837056 1103 9 0.8365633905541898 1103 10 0.6948222491421063 1103 11 0.7630033415308404 1103 12 1.048051279521495 1103 13 0.9726008855614623 1103 14 1.2661999008186058 1103 15 0.9902021335609703 1103 16 1.049413877645506 1103 17 1.0176509163652838 1103 18 1.0570560635974928 1103 19 1.1523115711588616 1103 20 1.0580339815044582 1103 21 1.1488191070929332 1103 22 1.1759345452845338 1103 23 1.0070706262199849 1103 24 1.2454770646808058 1103 25 1.0163410601846223 1104 1 1.4669589451134684 1104 2 3.9701109803208396 1104 3 2.4905506439592635 1104 4 1.4898752779345799 1104 5 1.1181079976227153 1104 6 1.020689065173407 1104 7 0.8594605232533752 1104 8 0.8994547330527638 1104 9 0.9001239096388076 1104 10 0.753842995116834 1104 11 0.8454986071256343 1104 12 0.7932844356328559 1104 13 0.7312349131647267 1104 14 1.0683879926192574 1104 15 1.1117108690107855 1104 16 0.8842500664041424 1104 17 0.8852296438162952 1104 18 0.8949911730924214 1104 19 1.0942348319116704 1104 20 0.9126199900025682 1104 21 0.9277289538345315 1104 22 1.1007654945800027 1104 23 1.0135205860617162 1104 24 0.9468752950890789 1104 25 1.0678988560623637 1105 1 1.6475937889474146 1105 2 4.023285749315729 1105 3 2.6290834119676063 1105 4 1.7467296339636462 1105 5 1.3053639145268505 1105 6 1.0826508256192966 1105 7 1.0132477974715997 1105 8 0.9683603924816353 1105 9 1.0356393157396653 1105 10 0.8982353283548647 1105 11 0.8963864962054799 1105 12 1.125512358581929 1105 13 0.9097450755045315 1105 14 1.039705577912848 1105 15 1.234948679387088 1105 16 0.9329794990159996 1105 17 1.1701089807585703 1105 18 1.1320482889295818 1105 19 1.0776081192063103 1105 20 1.1405327280375914 1105 21 1.2263888728374326 1105 22 1.1317768115526334 1105 23 1.2022044774413985 1105 24 1.1784856072955066 1105 25 1.2445545366987787 1106 1 1.3348593180824508 1106 2 3.823851907456767 1106 3 2.218791920952654 1106 4 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1107 17 1.0063367613501129 1107 18 0.9047619705322489 1107 19 1.0068401041706423 1107 20 1.142485412062804 1107 21 1.0807316115988215 1107 22 1.1877997738963444 1107 23 1.3237186313495144 1107 24 1.2799801733419969 1107 25 1.1990997138743467 1108 1 1.3611148963839668 1108 2 3.8445753337983533 1108 3 2.354918619897795 1108 4 1.4697572341271439 1108 5 1.059551744844157 1108 6 0.9424730223705069 1108 7 0.763422513709564 1108 8 0.6267197605590837 1108 9 0.6202998731737921 1108 10 0.6148337997128408 1108 11 0.6299924678564324 1108 12 0.6166314857582051 1108 13 0.6560106499029175 1108 14 0.7234538236046077 1108 15 0.7476139988212509 1108 16 0.7616096665435066 1108 17 0.7773661716358831 1108 18 0.7797553258851053 1108 19 0.8586461551814182 1108 20 0.7603266128977992 1108 21 0.7992877046324978 1108 22 0.8650344226064206 1108 23 0.9452847854564794 1108 24 0.9455238347796211 1108 25 0.9272955736485855 1109 1 1.060781956228329 1109 2 3.092901258593745 1109 3 1.9596233978291444 1109 4 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-1.987383831404813 2315 19 -2.0446955826243 2315 20 -2.0646970915286786 2315 21 -2.2925604726072777 2315 22 -2.0794067572980026 2315 23 -2.2107051601038776 2315 24 -2.233642712688379 2315 25 -2.2066107895498774 2316 1 -0.850385239655008 2316 2 1.5467210242147615 2316 3 0.17106753773924765 2316 4 -0.7393073033262212 2316 5 -1.0918059343695248 2316 6 -1.317612680897028 2316 7 -1.8520350075755907 2316 8 -1.7863916729576808 2316 9 -2.058757344637833 2316 10 -1.9337360954151137 2316 11 -1.9008269749001911 2316 12 -2.1113690599392 2316 13 -2.0370890269691984 2316 14 -2.376706057513516 2316 15 -2.3333485867362143 2316 16 -2.409036900097476 2316 17 -2.348864634215822 2316 18 -2.461870955148541 2316 19 -2.4116175497494083 2316 20 -2.3383679268138877 2316 21 -2.5157287112505813 2316 22 -2.42475132436315 2316 23 -2.483589441311622 2316 24 -2.6081776456172747 2316 25 -2.4346009766137726 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97649.0 3 5.0 4 909.0 5 4476.0 6 5035.0 7 4618.0 8 4132.0 9 4095.0 10 3896.0 11 3761.0 12 3665.0 13 3680.0 14 3823.0 15 3867.0 16 4037.0 17 4484.0 18 5030.0 19 6156.0 20 8026.0 21 10288.0 22 12719.0 23 15009.0 24 17569.0 25 20040.0 26 22759.0 27 25645.0 28 29600.0 29 35519.0 30 44312.0 31 55620.0 32 71275.0 33 94097.0 34 126947.0 35 179947.0 36 286857.0 37 363506.0 38 891358.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.427915168498686 19.19717460033923 13.358128459661714 25.01678177150037 2 16.058473698281496 21.178216638362088 40.59826911973956 22.165040543616858 3 18.619501772340975 24.943633050451197 29.77658117426733 26.6602840029405 4 11.993278400395084 17.26899856167791 39.101547802689204 31.6361752352378 5 12.955648839259121 37.906515934499154 36.15337144880135 12.98446377744037 6 30.838167143615188 37.797520298770095 18.64221424815845 12.72209830945627 7 27.35875325481498 31.599317979106946 22.900843877593495 18.141084888484574 8 24.21651051502762 37.959134517264914 21.00386718293768 16.82048778476979 9 26.04886577048033 15.698362155680684 20.72468963320968 37.528082440629305 10 14.77923433091754 29.42037519231849 34.83960425329503 20.96078622346894 11 33.866079644812444 22.51069042289256 25.258940410465357 18.364289521829637 12 23.16155238559803 24.55012526213309 32.5414007616358 19.746921590633086 13 28.912254269804006 22.247799577353963 26.05799925719697 22.781946895645063 14 20.496554533583954 22.644055494418673 28.126491516566972 28.732898455430405 15 22.504062582974292 31.667334165585263 23.701195961382325 22.12740729005812 16 21.569496740840545 28.325407541431076 28.56991017256228 21.5351855451661 17 21.232002282563407 28.816675968543628 28.436140964455785 21.515180784437185 18 21.03256088014481 27.73536813407312 30.61819560291318 20.613875382868894 19 23.14054663535954 26.971560897345228 27.913644060604348 21.97424840669089 20 23.118713908077517 27.713059794836028 28.66189165825726 20.5063346388292 21 22.601339636809218 27.02705542591705 27.539785370364235 22.831819566909502 22 22.613795218921595 28.519883170686462 27.897060232082477 20.96926137830947 23 21.997879068083208 28.058700670550728 28.262711042425103 21.680709218940958 24 22.849487352540606 27.015328906688868 28.932715273539955 21.202468467230574 25 22.5002636991187 27.637445840646524 28.506379902126206 21.355910558108576 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 240.0 1 240.0 2 441.5 3 643.0 4 643.0 5 643.0 6 1625.5 7 2608.0 8 2608.0 9 2608.0 10 3849.5 11 5091.0 12 5091.0 13 5091.0 14 8391.5 15 11692.0 16 11692.0 17 11692.0 18 21246.0 19 30800.0 20 30800.0 21 30800.0 22 49995.0 23 69190.0 24 69190.0 25 69190.0 26 99774.5 27 130359.0 28 130359.0 29 130359.0 30 168746.0 31 207133.0 32 207133.0 33 207133.0 34 230425.0 35 253717.0 36 253717.0 37 253717.0 38 268661.5 39 283606.0 40 283606.0 41 283606.0 42 296484.5 43 309363.0 44 309363.0 45 309363.0 46 314381.5 47 319400.0 48 319400.0 49 319400.0 50 303930.5 51 288461.0 52 288461.0 53 288461.0 54 254751.0 55 221041.0 56 221041.0 57 221041.0 58 187439.0 59 153837.0 60 153837.0 61 153837.0 62 125660.0 63 97483.0 64 97483.0 65 97483.0 66 74779.0 67 52075.0 68 52075.0 69 52075.0 70 37956.5 71 23838.0 72 23838.0 73 23838.0 74 16575.5 75 9313.0 76 9313.0 77 9313.0 78 6248.0 79 3183.0 80 3183.0 81 3183.0 82 2079.5 83 976.0 84 976.0 85 976.0 86 621.5 87 267.0 88 267.0 89 267.0 90 162.0 91 57.0 92 57.0 93 57.0 94 44.0 95 31.0 96 31.0 97 31.0 98 19.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.312703103890178 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.041365803821596E-5 20 0.0 21 3.6372292234394365E-4 22 0.0012932370572229107 23 0.001091168767031831 24 4.041365803821596E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2474411.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.20760171348391 #Duplication Level Percentage of deduplicated Percentage of total 1 83.80068924823672 60.510467925521105 2 11.400917251387826 16.464657841132 3 2.6516691644431085 5.744120127061041 4 0.9466773977535324 2.7342921795257786 5 0.3937849028087786 1.4217131711399629 6 0.22449766234904933 0.9726262673105008 7 0.12376979727405442 0.6255984158006499 8 0.07733928226674326 0.44675872725789656 9 0.05970260584586518 0.38798837857578145 >10 0.2819477757353638 3.6210941400346996 >50 0.01915669537454345 0.964541966083895 >100 0.016110933997749034 2.374015069437987 >500 0.0020951432346280464 1.1168532706453416 >1k 0.001528888306341574 2.1642788695427706 >5k 1.1325098565493141E-4 0.4509936509305668 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5785 0.23379301175107933 No Hit GTCCTACAGTGGACATTTCTAAATT 5245 0.2119696364104427 No Hit CTGTAGGACGTGGAATATGGCAAGA 4847 0.19588500051123278 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4612 0.18638779087225202 No Hit CTTTAGGACGTGAAATATGGCGAGG 4082 0.16496855211199757 No Hit GGTATCAACGCAGAGTACTTTTTTT 3563 0.14399386359016347 No Hit TATCAACGCAGAGTACTTTTTTTTT 3560 0.14387262261604883 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.0413658038215966E-5 0.0 0.0 0.0 0.0 6 4.0413658038215966E-5 0.0 0.0 0.0 0.0 7 4.0413658038215966E-5 0.0 0.0 0.0 0.0 8 4.0413658038215966E-5 0.0 0.0 0.0 0.0 9 4.0413658038215966E-5 0.0 0.0 0.0 4.0413658038215966E-5 10 4.0413658038215966E-5 0.0 0.0 0.0 4.0413658038215966E-5 11 8.082731607643193E-5 0.0 0.0 0.0 4.0413658038215966E-5 12 8.082731607643193E-5 0.0 0.0 0.0 4.0413658038215966E-5 13 8.082731607643193E-5 0.0 0.0 0.0 4.0413658038215966E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAACG 70 7.616998E-6 13.509696 4 CGGTAGG 110 1.5243131E-9 13.476758 1 GGTATCA 1480 0.0 12.887909 1 AGAACCG 85 4.1522617E-6 12.238195 5 TAGGACC 795 0.0 12.133237 4 CCCGTTT 260 0.0 12.002846 12 TCCAACG 450 0.0 11.979082 18 CCAACGT 160 3.6379788E-12 11.821463 19 CCAACGA 295 0.0 11.540954 19 ATCCCGT 315 0.0 11.408189 10 TCCCGTT 285 0.0 11.281782 11 GACTGCG 145 1.3151293E-9 11.087268 7 CGTTTCC 320 0.0 10.934411 14 AATGTCC 745 0.0 10.535749 8 CCGTTTC 300 0.0 10.402467 13 CGCGCCT 100 2.5237468E-5 10.402466 12 AGGACCT 1830 0.0 10.386964 5 TTAGGAC 1635 0.0 10.353303 3 GGCGAGG 1180 0.0 10.338771 19 GGACCTG 1795 0.0 10.220707 6 >>END_MODULE