FastQCFastQC Report
Thu 26 May 2016
SRR1511758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511758_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474411
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78040.3153881873302374No Hit
GTCCTACAGTGGACATTTCTAAATT59470.24034002435327034No Hit
CTGTAGGACGTGGAATATGGCAAGA53620.216698034400914No Hit
GTCCTAAAGTGTGTATTTCTCATTT51970.21002978082460835No Hit
TATCAACGCAGAGTACTTTTTTTTT49140.19859271559979327No Hit
GGTATCAACGCAGAGTACTTTTTTT46590.18828723280004817No Hit
CTTTAGGACGTGAAATATGGCGAGG46130.18642820453029024No Hit
CTGAAGGACCTGGAATATGGCGAGA26610.10754074403969269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3250.013.44180718
CCAACGA2500.013.295719
TAGCACG604.092153E-412.6671794
CGTGCCG604.1047303E-412.66257110
CCCGTTT2100.011.75810212
GGTATCA13350.011.5071711
GAACAGT6750.011.11492356
GGCGAGG12000.011.0797519
TAGGACC8250.010.9398364
GTATCAA30950.010.7285861
GCCGGTT2250.010.55214211
TCCCGTT2350.010.5072411
GTGCGCC1201.5309361E-610.28833911
AATCCCG4400.09.92860819
CACCTTT8900.09.92375814
TGTAGGA27450.09.89839652
AGAACAG8850.09.8761045
GTCCTAC27600.09.7836591
AGGACGT29700.09.6923115
GTAGGAC27250.09.6921353
Produced by FastQC (version 0.11.5)