FastQCFastQC Report
Thu 26 May 2016
SRR1511757_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511757_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571782
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51040.19846161144296057No Hit
TATCAACGCAGAGTACTTTTTTTTT32070.1246995274093994No Hit
GGTATCAACGCAGAGTACTTTTTTT29850.11606738051670008No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCGG803.0058593E-511.8206616
GGTATCA13550.011.673871
TATAACG600.004366677711.5340272
TCCAACG1701.2732926E-1111.12532618
GGTCGCC600.00608149611.03261711
AGCGACC600.00608149611.0326178
CCGCGTC600.00608149611.0326179
GGTCTAA1209.13471E-710.7101681
CTACACG1002.5246178E-510.4021814
GGACCGT1502.6271664E-810.0869646
TGGACCG1606.661139E-910.0475625
CGCCCTA1054.3192624E-59.90683816
GTACTAA1201.0604039E-59.8863091
GCACCGT1151.0844904E-59.86768156
GTATCAA25500.09.8087691
ACCGTGC1356.864084E-79.806778
GTCCTAA4800.09.474381
TATACCG800.0046912729.4565295
GACCGTG1804.5292836E-99.4565287
CTAGTAC1401.1274515E-69.4565283
Produced by FastQC (version 0.11.5)