##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511757_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2571782 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.61607904558007 31.0 30.0 34.0 25.0 34.0 2 26.930661696831223 31.0 26.0 34.0 10.0 34.0 3 28.60648414212402 31.0 28.0 34.0 16.0 34.0 4 33.139250138619836 35.0 33.0 37.0 28.0 37.0 5 33.58296037533508 35.0 35.0 37.0 28.0 37.0 6 33.83468000009332 37.0 35.0 37.0 30.0 37.0 7 33.881495398910175 37.0 35.0 37.0 30.0 37.0 8 33.906115681655756 37.0 35.0 37.0 30.0 37.0 9 35.5220454144247 39.0 35.0 39.0 30.0 39.0 10 35.40607796461753 39.0 35.0 39.0 30.0 39.0 11 35.420444267826745 39.0 35.0 39.0 30.0 39.0 12 35.32478297149603 39.0 35.0 39.0 30.0 39.0 13 35.31082339016293 39.0 35.0 39.0 30.0 39.0 14 36.48539067463727 40.0 37.0 41.0 30.0 41.0 15 36.41728964585645 40.0 36.0 41.0 29.0 41.0 16 36.35638907185757 40.0 36.0 41.0 29.0 41.0 17 36.320099837389016 40.0 36.0 41.0 29.0 41.0 18 36.25312720907137 40.0 36.0 41.0 29.0 41.0 19 36.28220510136551 40.0 37.0 41.0 29.0 41.0 20 36.20399435099865 40.0 36.0 41.0 29.0 41.0 21 36.12387597393558 40.0 36.0 41.0 28.0 41.0 22 36.017616578699126 40.0 36.0 41.0 27.0 41.0 23 35.90912137965037 40.0 36.0 41.0 27.0 41.0 24 35.78809090350582 40.0 36.0 41.0 27.0 41.0 25 35.67722264173246 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9544156107682795 1101 2 3.531014030361309 1101 3 2.055901911156269 1101 4 1.121356497839443 1101 5 0.7821790104014781 1101 6 0.5385316528679951 1101 7 0.392105861449231 1101 8 0.10989748708266234 1101 9 0.19480962037264504 1101 10 0.36952430219884036 1101 11 0.23788702322147515 1101 12 0.25722483696766574 1101 13 0.23669187675333347 1101 14 0.3560219953355457 1101 15 0.30522533626432136 1101 16 0.311976705894466 1101 17 0.4527818527450975 1101 18 0.46442323657201 1101 19 0.5255324772399774 1101 20 0.5452483908355106 1101 21 0.5316369788999609 1101 22 0.39915321845296603 1101 23 0.5112332549703282 1101 24 0.4348233423406782 1101 25 0.5116589237044096 1102 1 1.4599994809568209 1102 2 4.0951169653117 1102 3 2.663109664260851 1102 4 1.6547160274780168 1102 5 1.2103509202736689 1102 6 0.9674075571382801 1102 7 0.8602007151149422 1102 8 0.8853537028761025 1102 9 0.9008352338977517 1102 10 0.9064721746819373 1102 11 0.8699581003591987 1102 12 0.8823900821842443 1102 13 0.9397581968574045 1102 14 0.9706863038338582 1102 15 1.072372990636886 1102 16 1.0341539945242815 1102 17 1.0079597615962257 1102 18 0.9921280833518011 1102 19 1.0220211455222525 1102 20 0.9749160070556329 1102 21 1.1224443673099032 1102 22 0.7714064100006723 1102 23 0.9032131283940927 1102 24 0.6973135140304123 1102 25 0.6927329321485871 1103 1 1.4484789998689855 1103 2 4.054754147329511 1103 3 2.658107857815345 1103 4 1.6046413580302428 1103 5 1.0886902280728705 1103 6 0.8184985683200452 1103 7 0.7887901496383449 1103 8 0.6448891415147457 1103 9 0.6883986983781227 1103 10 0.5414493279240276 1103 11 0.7811399525437253 1103 12 0.8813975837108501 1103 13 0.8462128951173042 1103 14 1.0900369299014585 1103 15 0.6805231995384275 1103 16 0.7732255452371675 1103 17 0.938646675013338 1103 18 1.0212683699987508 1103 19 1.0484456665866801 1103 20 0.7931919573051189 1103 21 0.8382088577785112 1103 22 1.001410150298419 1103 23 0.9742256388255157 1103 24 1.1344065291381256 1103 25 0.9093070924244415 1104 1 1.1636893304601088 1104 2 3.767217907863479 1104 3 2.276372984658927 1104 4 1.5102613635233837 1104 5 0.9233597895371872 1104 6 0.7729063772982769 1104 7 0.6636442108563401 1104 8 0.6184880638129684 1104 9 0.678782215470072 1104 10 0.606936277356624 1104 11 0.6559015894994786 1104 12 0.6705848169556816 1104 13 0.5417272666541209 1104 14 0.7515191186126202 1104 15 0.7016386557524328 1104 16 0.6292329043010625 1104 17 0.7881782322313668 1104 18 0.5290664491435706 1104 19 0.7296081196661035 1104 20 0.677997321400035 1104 21 0.6618467988100889 1104 22 0.8141019197434858 1104 23 0.8506448090125858 1104 24 0.7675414990784404 1104 25 0.7594359196390243 1105 1 1.4736901957452027 1105 2 3.8637491130392156 1105 3 2.471913272000137 1105 4 1.6372379575615668 1105 5 1.1059790407993333 1105 6 0.9408146171419887 1105 7 0.8503251436336683 1105 8 0.7004838106039273 1105 9 0.8156929480004678 1105 10 0.6678931162704913 1105 11 0.8018958187135041 1105 12 0.8926203003006066 1105 13 0.7421673972456091 1105 14 0.8134472400453987 1105 15 0.8712192254428288 1105 16 0.8262072352859278 1105 17 0.9364376779829868 1105 18 0.9240093569674102 1105 19 0.811453439971352 1105 20 0.8462947731354333 1105 21 0.9607275858201589 1105 22 0.953518151826259 1105 23 0.9614983129180175 1105 24 0.8373200859347563 1105 25 0.9074695592610951 1106 1 1.3039508811208513 1106 2 3.790701970354956 1106 3 2.3579601229056557 1106 4 1.535174049849779 1106 5 1.0065996088992293 1106 6 0.8338153425054315 1106 7 0.7044888560096183 1106 8 0.5796881529843816 1106 9 0.509239143703077 1106 10 0.5239777053784564 1106 11 0.601916013069328 1106 12 0.49869375281528505 1106 13 0.6722130721268726 1106 14 0.8151745256641192 1106 15 0.7895041009839758 1106 16 0.6098208148023119 1106 17 0.552224281552192 1106 18 0.7326060274229889 1106 19 0.5703395133644733 1106 20 0.6005775356936098 1106 21 0.6453318395533358 1106 22 0.5616279304233629 1106 23 0.5284750842117418 1106 24 0.49960586428240106 1106 25 0.5448375813501585 1107 1 1.350593518471058 1107 2 3.686247194081762 1107 3 2.374938115093272 1107 4 1.3224762703536115 1107 5 0.9818613333313948 1107 6 0.8916412500332527 1107 7 0.8063056420227994 1107 8 0.7044968344990608 1107 9 0.8422140591657055 1107 10 0.6827790255271751 1107 11 0.7643434999677581 1107 12 0.7831633619692511 1107 13 0.8150887680461167 1107 14 0.7724388229647658 1107 15 0.9150912668580773 1107 16 0.9989690330890326 1107 17 0.8961517500304552 1107 18 0.9225186265522964 1107 19 1.0441324625735788 1107 20 1.0808930823463 1107 21 1.1373190697305446 1107 22 1.0648482954551213 1107 23 1.1481220274140114 1107 24 1.0241666364852975 1107 25 1.0678002650819565 1108 1 1.513362424088573 1108 2 4.106669162526483 1108 3 2.6696984411129634 1108 4 1.5763528274318759 1108 5 1.2061453145010148 1108 6 1.0189049702589514 1108 7 0.9180210401265683 1108 8 0.9053144596166263 1108 9 0.8933671468094104 1108 10 0.8400641130728701 1108 11 0.8187525751416445 1108 12 0.8663887906507171 1108 13 0.9175460412594418 1108 14 1.0063836474053147 1108 15 0.9796537456933763 1108 16 1.0113022049580564 1108 17 1.070731738633718 1108 18 1.06818833591025 1108 19 0.9723854501345457 1108 20 1.0332912671607346 1108 21 0.8717451457770906 1108 22 0.9821861070671076 1108 23 1.100118122113777 1108 24 1.1575113414296894 1108 25 1.1145696280769712 1109 1 0.9602256586161246 1109 2 3.1451272505457766 1109 3 1.8394885924322217 1109 4 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5 -0.13006750308616688 2314 6 -0.24148991443694 2314 7 -0.3897271171409784 2314 8 -0.37805672884079655 2314 9 -0.6237483589243524 2314 10 -0.6163943478655796 2314 11 -0.7372378773225563 2314 12 -0.6716050761572916 2314 13 -0.8350476839037029 2314 14 -0.7897592540333847 2314 15 -0.8434719419741015 2314 16 -0.679143945809713 2314 17 -0.7171252947566913 2314 18 -0.6672786822719203 2314 19 -0.759743596379657 2314 20 -0.7248699870123616 2314 21 -0.8549347485442311 2314 22 -0.8052070928593693 2314 23 -0.8760331497114748 2314 24 -0.6724033203410187 2314 25 -0.7007426805153543 2315 1 -1.7372916571658301 2315 2 -3.831549651189281 2315 3 -1.8773849059898247 2315 4 -1.8534463792936684 2315 5 -1.5492398242707779 2315 6 -1.5889015564383016 2315 7 -1.549024357070408 2315 8 -1.5248620942890767 2315 9 -1.7089030332592259 2315 10 -1.818231363858871 2315 11 -1.7959815897900668 2315 12 -1.8951613505406613 2315 13 -1.7682312234832906 2315 14 -1.940474572269153 2315 15 -2.0181008720703275 2315 16 -2.131730820989752 2315 17 -2.08799872506782 2315 18 -2.0773559829140424 2315 19 -2.0950012502858684 2315 20 -2.02033609468549 2315 21 -2.198116214358393 2315 22 -1.9523139284132611 2315 23 -1.9312166801324793 2315 24 -1.9797788600706312 2315 25 -1.887470429132243 2316 1 -0.34523532265816925 2316 2 1.9798157688724025 2316 3 0.6007940809189414 2316 4 -0.3058135770150514 2316 5 -0.5482713536961157 2316 6 -0.7461596561952462 2316 7 -1.1264739266827348 2316 8 -1.037222684779728 2316 9 -1.2815127626686618 2316 10 -1.263840461386316 2316 11 -1.2882565484730293 2316 12 -1.304477218119871 2316 13 -1.386476072945932 2316 14 -1.6679195316775122 2316 15 -1.7010824898105668 2316 16 -1.70707684847968 2316 17 -1.6234924888383446 2316 18 -1.7118756171391496 2316 19 -1.6062381308689098 2316 20 -1.355426039546579 2316 21 -1.8322189637879092 2316 22 -1.5260044338401926 2316 23 -1.6344624385263273 2316 24 -1.597878957868133 2316 25 -1.673669816302855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75251.0 3 2.0 4 655.0 5 3376.0 6 4072.0 7 3776.0 8 3532.0 9 3433.0 10 3290.0 11 3160.0 12 3223.0 13 3158.0 14 3289.0 15 3419.0 16 3577.0 17 3913.0 18 4433.0 19 5335.0 20 6934.0 21 8800.0 22 11070.0 23 13262.0 24 15472.0 25 17900.0 26 20449.0 27 23534.0 28 27410.0 29 34098.0 30 42896.0 31 55582.0 32 72214.0 33 97680.0 34 135048.0 35 194246.0 36 309586.0 37 396956.0 38 957751.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.674813806146865 18.203175852385623 12.858360467566845 25.26364987390067 2 17.227969901582565 20.68717402527714 38.25393405503409 23.830922018106207 3 18.7533780079338 24.12016259542994 29.211418386161814 27.91504101047445 4 12.949620146653176 16.338554356473452 37.02199486581678 33.6898306310566 5 14.158820615433191 36.951926718516575 34.80730481821554 14.081947847834691 6 32.77598956676732 35.91809103570987 17.66487206147333 13.641047336049478 7 28.78568245675567 31.109246429129684 21.80631950919635 18.2987516049183 8 26.185112112924035 35.459031908614335 20.15894037675044 18.196915601711186 9 26.698063832782093 15.49198182427593 19.686621960959368 38.123332381982614 10 15.813937573246879 28.353841810853332 33.185899893536856 22.646320722362937 11 34.94588577103347 22.468622923715927 23.373676306934257 19.211814998316342 12 23.410032421099455 24.26718127741776 30.63459500066491 21.688191300817877 13 29.018789306403107 21.189820910170457 25.55912592902509 24.232263854401346 14 22.180418091424546 20.748453795850505 26.835439395718613 30.235688717006344 15 23.901287123092082 28.757064167958248 23.30524126850565 24.03640744044402 16 23.711029939551644 27.336414983851665 25.61966760790767 23.332887468689027 17 22.865118427611673 27.325060988839645 26.707201465754093 23.10261911779459 18 22.95630033960888 26.92825441658741 27.625436370578843 22.490008873224866 19 24.093381201587537 26.466872568076365 26.75779936160972 22.68194686872638 20 24.140002589645075 26.33144112970739 27.061013360002274 22.467542920645265 21 24.145989329556954 25.99424444654416 26.52302279707766 23.336743426821222 22 24.18260193999591 26.21385838014308 26.605769283112952 22.99777039674806 23 23.889868759545962 26.115574966326665 26.594622843118042 23.39993343100933 24 23.801948921778333 26.144994461036923 26.862901623427497 23.190154993757243 25 23.853771431637675 26.061462441217802 26.708095787279014 23.37667033986551 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 270.0 1 270.0 2 423.5 3 577.0 4 577.0 5 577.0 6 1446.5 7 2316.0 8 2316.0 9 2316.0 10 3253.5 11 4191.0 12 4191.0 13 4191.0 14 6839.0 15 9487.0 16 9487.0 17 9487.0 18 17210.5 19 24934.0 20 24934.0 21 24934.0 22 38876.5 23 52819.0 24 52819.0 25 52819.0 26 75347.5 27 97876.0 28 97876.0 29 97876.0 30 126260.5 31 154645.0 32 154645.0 33 154645.0 34 179901.0 35 205157.0 36 205157.0 37 205157.0 38 231269.5 39 257382.0 40 257382.0 41 257382.0 42 280178.0 43 302974.0 44 302974.0 45 302974.0 46 317794.5 47 332615.0 48 332615.0 49 332615.0 50 332665.5 51 332716.0 52 332716.0 53 332716.0 54 309342.5 55 285969.0 56 285969.0 57 285969.0 58 251083.5 59 216198.0 60 216198.0 61 216198.0 62 180486.0 63 144774.0 64 144774.0 65 144774.0 66 113650.5 67 82527.0 68 82527.0 69 82527.0 70 60982.5 71 39438.0 72 39438.0 73 39438.0 74 27910.0 75 16382.0 76 16382.0 77 16382.0 78 11174.5 79 5967.0 80 5967.0 81 5967.0 82 3901.0 83 1835.0 84 1835.0 85 1835.0 86 1174.5 87 514.0 88 514.0 89 514.0 90 342.5 91 171.0 92 171.0 93 171.0 94 104.5 95 38.0 96 38.0 97 38.0 98 24.0 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.349202226316227 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 3.888354456170858E-5 20 3.888354456170858E-5 21 1.9441772280854287E-4 22 9.332050694810058E-4 23 5.4436962386392E-4 24 3.888354456170858E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2571782.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.84879464634326 #Duplication Level Percentage of deduplicated Percentage of total 1 79.35251104117117 54.63324737345281 2 13.193118213959142 18.16660573315608 3 3.811622968592528 7.872769411017368 4 1.5080397852554834 4.153068859742816 5 0.7333406927881674 2.5244811381789822 6 0.4174263501754285 1.7243580637920393 7 0.24827937556628038 1.1965615020299623 8 0.1534302065296999 0.8450787825527476 9 0.11463586830692818 0.7103287220552053 >10 0.442253254670689 4.991683659850864 >50 0.014843455660877393 0.69300899212972 >100 0.008791850938022799 1.1131518141006262 >500 9.672639518310403E-4 0.4384298593207608 >1k 6.827745541997372E-4 0.7372845230476823 >5k 5.689787951664477E-5 0.19994156557225284 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5104 0.19846161144296057 No Hit TATCAACGCAGAGTACTTTTTTTTT 3207 0.1246995274093994 No Hit GGTATCAACGCAGAGTACTTTTTTT 2985 0.11606738051670008 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.888354456170857E-5 7 0.0 0.0 0.0 0.0 3.888354456170857E-5 8 0.0 0.0 0.0 0.0 3.888354456170857E-5 9 0.0 0.0 0.0 0.0 3.888354456170857E-5 10 3.888354456170857E-5 0.0 0.0 0.0 3.888354456170857E-5 11 3.888354456170857E-5 0.0 0.0 0.0 3.888354456170857E-5 12 3.888354456170857E-5 0.0 0.0 0.0 3.888354456170857E-5 13 3.888354456170857E-5 0.0 0.0 0.0 3.888354456170857E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCCGG 80 3.0058593E-5 11.820661 6 GGTATCA 1355 0.0 11.67387 1 TATAACG 60 0.0043666777 11.534027 2 TCCAACG 170 1.2732926E-11 11.125326 18 GGTCGCC 60 0.006081496 11.032617 11 AGCGACC 60 0.006081496 11.032617 8 CCGCGTC 60 0.006081496 11.032617 9 GGTCTAA 120 9.13471E-7 10.710168 1 CTACACG 100 2.5246178E-5 10.402181 4 GGACCGT 150 2.6271664E-8 10.086964 6 TGGACCG 160 6.661139E-9 10.047562 5 CGCCCTA 105 4.3192624E-5 9.906838 16 GTACTAA 120 1.0604039E-5 9.886309 1 GCACCGT 115 1.0844904E-5 9.8676815 6 GTATCAA 2550 0.0 9.808769 1 ACCGTGC 135 6.864084E-7 9.80677 8 GTCCTAA 480 0.0 9.47438 1 TATACCG 80 0.004691272 9.456529 5 GACCGTG 180 4.5292836E-9 9.456528 7 CTAGTAC 140 1.1274515E-6 9.456528 3 >>END_MODULE