FastQCFastQC Report
Thu 26 May 2016
SRR1511757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511757_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571782
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64640.25134323204688425No Hit
TATCAACGCAGAGTACTTTTTTTTT42810.16646045426867442No Hit
GGTATCAACGCAGAGTACTTTTTTT39600.15397883646436594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAAAC551.9599502E-413.8190033
TAAACCG752.073678E-411.4006775
GGTATCA15000.011.1862711
GTATCAA30600.010.0945631
TATACTG4050.010.0870185
CGAATTT856.612539E-410.05609615
ATAAGAC2650.09.679823
ACCGCGG800.00452726339.4974248
CCCGTTT1606.667324E-89.49742412
ATTATAC2550.09.3142783
TAGGACA5450.09.239084
GTCCTAA5250.09.0797651
TCCAACG2002.6702764E-99.022552518
TTAGGAC7300.08.9799853
GAATAGG2252.4374458E-108.89816951
ATAGGAC2900.08.8453523
GTCTTAC1951.5528713E-88.8003881
TAGGACG10500.08.7767114
GTGTATA7950.08.7542651
TAAGACA3800.08.750524