FastQCFastQC Report
Thu 26 May 2016
SRR1511756_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511756_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435009
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55560.22817164125471406No Hit
TATCAACGCAGAGTACTTTTTTTTT32520.13355186777543737No Hit
GGTATCAACGCAGAGTACTTTTTTT32330.13277158318511348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTAC550.00317581712.0359483
ACACCGT1354.0199666E-1011.9085856
GGTATCA12550.011.6555821
TTAGGAC8800.011.2837023
GACGTGA7750.010.8600867
CCGTGCG803.9344863E-410.6389199
CGTCTAA750.001906081110.5425271
GTATAAG2550.010.4650091
TAGGACG15600.010.4267474
TAGGACC4600.010.2791494
GTACTAG1353.7472637E-710.2496791
AGGACGT15550.010.2170115
ATAGGAC2800.010.1323053
ACTGTTC10400.010.0933348
GGACGTG15200.010.0789776
GTATCAA25500.09.9998971
TCTAGAC2850.09.9545443
AGGACCT10500.09.9071425
ACGTGAA9550.09.9024278
GTAGGAC14550.09.7492963
Produced by FastQC (version 0.11.5)