FastQCFastQC Report
Thu 26 May 2016
SRR1511756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511756_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435009
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72020.2957689273427737No Hit
TATCAACGCAGAGTACTTTTTTTTT45780.1880075186580419No Hit
GGTATCAACGCAGAGTACTTTTTTT43340.1779870218138824No Hit
CTGTAGGACGTGGAATATGGCAAGA24360.10004069800152689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14650.012.6969781
TAGGACC4450.012.596054
TCCAACG2450.012.40436918
CCGTGTA600.005890674411.0799449
TACACCG700.001491389810.8576135
CGTGCCA700.001495739910.85382310
CGTTTCC2200.010.79215214
GTCCTAA8100.010.5989011
AGGACGT14600.010.2161965
GGACGTG14450.010.1214716
GACCGTC856.6143787E-410.0557477
GTATCAA30400.09.9783081
CCGTTTC2201.8189894E-129.92878113
TTAGGAC8350.09.8986323
CCCGTTT2600.09.86236712
AGGACCT9600.09.7972985
CCAACGA1951.6370905E-109.74080919
GACGTGA6450.09.7179577
CTACACT5100.09.6866934
TGTAGGA16700.09.6141892
Produced by FastQC (version 0.11.5)