##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511756_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2435009 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.374639272380513 33.0 31.0 34.0 28.0 34.0 2 31.555638603389145 33.0 31.0 34.0 28.0 34.0 3 31.66849280639209 34.0 31.0 34.0 28.0 34.0 4 35.21763779928534 37.0 35.0 37.0 32.0 37.0 5 34.909407316358994 37.0 35.0 37.0 32.0 37.0 6 34.87600579710383 37.0 35.0 37.0 32.0 37.0 7 34.725989103120355 37.0 35.0 37.0 32.0 37.0 8 34.65128424576665 37.0 35.0 37.0 32.0 37.0 9 36.21968296626419 39.0 37.0 39.0 32.0 39.0 10 36.05157968615311 39.0 37.0 39.0 31.0 39.0 11 36.05547125287833 39.0 37.0 39.0 31.0 39.0 12 35.90490343156843 39.0 37.0 39.0 30.0 39.0 13 35.885999189325375 39.0 37.0 39.0 30.0 39.0 14 37.01057655228379 40.0 37.0 41.0 31.0 41.0 15 36.938798583495995 40.0 37.0 41.0 31.0 41.0 16 36.79575845510222 40.0 37.0 41.0 30.0 41.0 17 36.77126285775535 40.0 37.0 41.0 30.0 41.0 18 36.70803105861211 40.0 36.0 41.0 30.0 41.0 19 36.726317644000495 40.0 37.0 41.0 30.0 41.0 20 36.824609272491394 40.0 37.0 41.0 31.0 41.0 21 36.77408091715472 40.0 37.0 41.0 30.0 41.0 22 36.68057612928741 40.0 37.0 41.0 30.0 41.0 23 36.593141544856714 40.0 37.0 41.0 30.0 41.0 24 36.45610632239963 40.0 37.0 41.0 30.0 41.0 25 35.96782475957994 39.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19021848829918397 1101 2 0.2734543746010232 1101 3 0.32673902702701696 1101 4 0.25114641301313867 1101 5 0.3580025607600348 1101 6 0.32972014321000387 1101 7 0.2711301187670685 1101 8 0.2292201478612128 1101 9 0.1902241844714183 1101 10 0.16465580070168073 1101 11 0.2425771716604217 1101 12 0.39130451750570927 1101 13 0.22696207764294485 1101 14 0.10516901401084056 1101 15 0.08296886083211774 1101 16 0.0547416789303341 1101 17 0.0060432160202879 1101 18 0.09984547759471951 1101 19 0.17120611765038518 1101 20 0.03747055579183467 1101 21 0.3259565666172435 1101 22 -0.035772037364068865 1101 23 -0.07636377846947084 1101 24 -0.36416456887045 1101 25 -0.10022828534268058 1102 1 0.4127395692201752 1102 2 0.34869889583811187 1102 3 0.31272316397040356 1102 4 0.33287504747072916 1102 5 0.4196826984080815 1102 6 0.4080852343580048 1102 7 0.47540862074882995 1102 8 0.3782724133115707 1102 9 0.4569045104132101 1102 10 0.5501456506049678 1102 11 0.5520074118062226 1102 12 0.6006092923159585 1102 13 0.7047658619429953 1102 14 0.7348492571884933 1102 15 0.6085145775962033 1102 16 0.6846642572227637 1102 17 0.8042555105168105 1102 18 0.8109322346296182 1102 19 0.7606778802904799 1102 20 0.9724713419448179 1102 21 0.8198040418747041 1102 22 0.7825200298523356 1102 23 0.910704802793532 1102 24 0.8650042387067458 1102 25 0.9929288220653874 1103 1 0.23131616863157234 1103 2 0.28052909068893683 1103 3 0.2366755417839137 1103 4 0.3390979114432824 1103 5 0.3769287769113916 1103 6 0.33307029956738177 1103 7 0.2832021147767776 1103 8 0.3893938056942261 1103 9 0.32091106389468393 1103 10 0.5731512393881033 1103 11 0.3586189741739503 1103 12 0.4150024920839712 1103 13 0.40626862731484437 1103 14 0.6823262621711805 1103 15 0.6124374216280657 1103 16 0.7255406029603719 1103 17 0.8039483160726775 1103 18 0.6542124908986082 1103 19 0.7367838735670276 1103 20 0.5819786990651963 1103 21 0.7211141992320478 1103 22 0.7802608448006367 1103 23 0.7758756688206887 1103 24 0.7092618376235649 1103 25 0.8426638927677885 1104 1 0.17545491244726819 1104 2 0.23527343283434377 1104 3 0.25007073313097195 1104 4 0.250451309745479 1104 5 0.20904229892961723 1104 6 0.38414144824796637 1104 7 0.44229745628206985 1104 8 0.45339770621887965 1104 9 0.5983400677652995 1104 10 0.5326288562393273 1104 11 0.38206818659102026 1104 12 0.5551437963667709 1104 13 0.4274140175766945 1104 14 0.5995280544981512 1104 15 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-1.2101468729990046 2314 20 -0.9125210434400159 2314 21 -1.1153639474808799 2314 22 -1.0449170798758018 2314 23 -1.1900548160293312 2314 24 -1.0963042709970168 2314 25 -1.2189828919243553 2315 1 -0.8155039449834334 2315 2 -0.829347957745977 2315 3 -0.8609193849483496 2315 4 -0.8043089018194749 2315 5 -0.9392665147954418 2315 6 -1.0250167365602465 2315 7 -1.107330675338595 2315 8 -1.0634741547527184 2315 9 -1.2079246551112774 2315 10 -1.1830333213389324 2315 11 -1.1033882040633785 2315 12 -1.2471059332508432 2315 13 -1.2427046543642817 2315 14 -1.5347290235942097 2315 15 -1.404507219854139 2315 16 -1.553344527054243 2315 17 -1.5981105769994741 2315 18 -1.4917809735103091 2315 19 -1.6035691574800666 2315 20 -1.496379279145458 2315 21 -1.6935511700167183 2315 22 -1.6811949308309408 2315 23 -1.5743371319650379 2315 24 -1.5743030908668842 2315 25 -1.569687949990012 2316 1 -0.827744519717136 2316 2 -0.9429590895922608 2316 3 -0.8643302829019035 2316 4 -0.8731569461912372 2316 5 -0.9668920964169914 2316 6 -0.9887894896405527 2316 7 -1.0896028957134973 2316 8 -1.6936931407802334 2316 9 -1.3967766353955184 2316 10 -1.3158152876212839 2316 11 -1.3234478987360987 2316 12 -1.309329203541715 2316 13 -1.4364429342800449 2316 14 -1.4470978547865414 2316 15 -1.465596653286326 2316 16 -1.4461154546854758 2316 17 -1.3931246354455098 2316 18 -1.439251018728065 2316 19 -1.523469153634629 2316 20 -1.5552763640819975 2316 21 -1.5843315212177416 2316 22 -1.5403019311001245 2316 23 -1.5048012077155235 2316 24 -1.6095508837021626 2316 25 -1.4752112340792038 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25235.0 3 0.0 4 451.0 5 4102.0 6 5497.0 7 4351.0 8 3675.0 9 3282.0 10 3082.0 11 3090.0 12 2980.0 13 2893.0 14 2856.0 15 2868.0 16 2893.0 17 3085.0 18 3354.0 19 3767.0 20 4713.0 21 6044.0 22 7691.0 23 9689.0 24 12126.0 25 14532.0 26 17190.0 27 19678.0 28 23057.0 29 28460.0 30 36964.0 31 47847.0 32 62625.0 33 82395.0 34 110514.0 35 149423.0 36 222353.0 37 406838.0 38 1095409.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.18780678958875 19.282308295177987 13.781050200057749 24.748834715175512 2 15.035426973781204 20.58850706506629 40.44789978188992 23.92816617926258 3 18.68005415996409 24.868409110602876 29.937261012176958 26.514275717256076 4 12.84295047780111 17.375007648842367 37.89953137750209 31.882510495854433 5 13.57688373258618 37.40580823538595 35.54350014173442 13.473807890293452 6 31.674872659608237 37.14047874155702 18.111965910598276 13.072682688236469 7 27.75217668599993 31.59318918328433 22.373716072507328 18.28091805820841 8 25.176334050510697 36.604300025174446 20.700128829092623 17.519237095222234 9 26.213989352811428 15.663104325281754 20.258364548139244 37.86454177376758 10 15.16914311199671 29.186052289745128 33.958313911776095 21.686490686482063 11 34.49079654325713 22.77650719155453 24.065249861499485 18.667446403688857 12 22.93515136905038 24.709436392226888 31.715529593525115 20.639882645197616 13 28.795745724143114 21.826654439470243 25.940561205317923 23.437038631068717 14 21.290763196357794 21.66094663305146 27.691725164054837 29.356565006535913 15 23.303938507003465 29.81832921356759 23.74722228952747 23.130509989901473 16 22.786897296888842 28.07213443564274 26.859325776619308 22.28164249084911 17 22.028912418804204 27.9058106150737 27.626181258467632 22.43909570765447 18 21.82217806997839 27.555421766408255 28.990200857573832 21.632199306039528 19 23.35765494090576 26.83928478293099 27.615380477033145 22.187679799130105 20 23.419092085491265 27.117107164696314 27.98884932252817 21.474951427284253 21 23.462500549279284 26.577109160582157 27.27180885163053 22.68858143850803 22 23.07441163461819 27.25045369442166 27.618953359104626 22.05618131185552 23 22.974904815546886 27.008319065761157 27.468892312102337 22.547883806589628 24 23.04656779502663 26.713207220178653 27.944619506539816 22.295605478254906 25 23.220605858499408 26.815246915963233 27.734153936125388 22.229993289411972 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 232.0 1 232.0 2 449.5 3 667.0 4 667.0 5 667.0 6 1666.0 7 2665.0 8 2665.0 9 2665.0 10 3808.0 11 4951.0 12 4951.0 13 4951.0 14 7861.0 15 10771.0 16 10771.0 17 10771.0 18 19608.0 19 28445.0 20 28445.0 21 28445.0 22 45200.5 23 61956.0 24 61956.0 25 61956.0 26 87551.5 27 113147.0 28 113147.0 29 113147.0 30 145862.5 31 178578.0 32 178578.0 33 178578.0 34 200572.0 35 222566.0 36 222566.0 37 222566.0 38 242446.5 39 262327.0 40 262327.0 41 262327.0 42 278683.0 43 295039.0 44 295039.0 45 295039.0 46 302983.0 47 310927.0 48 310927.0 49 310927.0 50 304686.0 51 298445.0 52 298445.0 53 298445.0 54 271122.5 55 243800.0 56 243800.0 57 243800.0 58 211070.0 59 178340.0 60 178340.0 61 178340.0 62 146476.0 63 114612.0 64 114612.0 65 114612.0 66 88293.0 67 61974.0 68 61974.0 69 61974.0 70 45406.5 71 28839.0 72 28839.0 73 28839.0 74 20058.0 75 11277.0 76 11277.0 77 11277.0 78 7607.5 79 3938.0 80 3938.0 81 3938.0 82 2538.0 83 1138.0 84 1138.0 85 1138.0 86 723.0 87 308.0 88 308.0 89 308.0 90 181.5 91 55.0 92 55.0 93 55.0 94 32.0 95 9.0 96 9.0 97 9.0 98 6.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4397930356725581 2 0.0 3 0.0 4 0.0 5 0.03609842920498446 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0020944481108694053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2435009.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.79115049286891 #Duplication Level Percentage of deduplicated Percentage of total 1 80.3090003721386 56.04857530903791 2 13.267437934512467 18.518995150847147 3 3.585751798403023 7.507612301772622 4 1.2624360736796953 3.5242746402322465 5 0.5582715578911739 1.948120715633565 6 0.2968620987973025 1.2431008447674876 7 0.16938572915768207 0.8275137440491687 8 0.1091800966424908 0.6095843644481638 9 0.07811724311202731 0.49067030431075725 >10 0.3268250755219288 3.871259922156186 >50 0.020295223494961847 0.9677562237102793 >100 0.013378505318691055 1.8083789402144972 >500 0.00194083872866255 0.9037202420363823 >1k 0.0010586393065258341 1.434820583195759 >5k 5.881329480699078E-5 0.2956167135878206 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7202 0.2957689273427737 No Hit TATCAACGCAGAGTACTTTTTTTTT 4578 0.1880075186580419 No Hit GGTATCAACGCAGAGTACTTTTTTT 4334 0.1779870218138824 No Hit CTGTAGGACGTGGAATATGGCAAGA 2436 0.10004069800152689 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.213522003409432E-5 0.0 9 0.0 0.0 0.0 8.213522003409432E-5 0.0 10 0.0 0.0 0.0 8.213522003409432E-5 0.0 11 0.0 0.0 0.0 8.213522003409432E-5 0.0 12 4.106761001704716E-5 0.0 0.0 2.0533805008523582E-4 8.213522003409432E-5 13 4.106761001704716E-5 0.0 0.0 2.0533805008523582E-4 8.213522003409432E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1465 0.0 12.696978 1 TAGGACC 445 0.0 12.59605 4 TCCAACG 245 0.0 12.404369 18 CCGTGTA 60 0.0058906744 11.079944 9 TACACCG 70 0.0014913898 10.857613 5 CGTGCCA 70 0.0014957399 10.853823 10 CGTTTCC 220 0.0 10.792152 14 GTCCTAA 810 0.0 10.598901 1 AGGACGT 1460 0.0 10.216196 5 GGACGTG 1445 0.0 10.121471 6 GACCGTC 85 6.6143787E-4 10.055747 7 GTATCAA 3040 0.0 9.978308 1 CCGTTTC 220 1.8189894E-12 9.928781 13 TTAGGAC 835 0.0 9.898632 3 CCCGTTT 260 0.0 9.862367 12 AGGACCT 960 0.0 9.797298 5 CCAACGA 195 1.6370905E-10 9.740809 19 GACGTGA 645 0.0 9.717957 7 CTACACT 510 0.0 9.686693 4 TGTAGGA 1670 0.0 9.614189 2 >>END_MODULE