FastQCFastQC Report
Thu 26 May 2016
SRR1511755_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511755_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276168
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55040.2418099191272349No Hit
GTCCTACAGTGGACATTTCTAAATT52410.2302554117270781No Hit
GTCCTAAAGTGTGTATTTCTCATTT46690.2051254564689425No Hit
CTGTAGGACGTGGAATATGGCAAGA45970.20196224531756884No Hit
CTTTAGGACGTGAAATATGGCGAGG40400.17749129238263606No Hit
TATCAACGCAGAGTACTTTTTTTTT34240.15042826364310544No Hit
GGTATCAACGCAGAGTACTTTTTTT34050.14959352736704848No Hit
CTGAAGGACCTGGAATATGGCGAGA23410.10284829590785918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3800.014.43443418
CCAACGA2200.013.32583219
GGTATCA10100.012.6228431
GGCGAGG10850.011.8539919
TACACCG803.0045068E-511.8210445
GACCGTC658.338412E-411.6391817
ACCGTCT658.338412E-411.6391818
CGTTAGG802.6164638E-411.1183761
CGTTTAC600.006079661711.03297514
TAGGACC6950.011.0216354
CCGAATG700.001549332210.80804918
CCAACGT2100.010.80804919
TGTGCGA700.00154961610.80781210
GTATCAA20950.010.70854951
TGTAGGA22200.010.6398082
TATCGCG803.934206E-410.6389397
CGGTAGG750.00190698910.5418661
AGGACGT25950.010.4590045
GTATTAG1801.9099389E-1010.4320561
GTCCTAA14500.010.4282691
Produced by FastQC (version 0.11.5)