##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511755_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2276168 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.402230854664506 31.0 30.0 34.0 25.0 34.0 2 26.7303705174662 31.0 26.0 34.0 10.0 34.0 3 28.401825787903178 31.0 28.0 34.0 16.0 34.0 4 32.89800972511695 35.0 33.0 37.0 27.0 37.0 5 33.35433632315365 35.0 35.0 37.0 28.0 37.0 6 33.60426295422833 37.0 35.0 37.0 28.0 37.0 7 33.64427933263274 37.0 35.0 37.0 28.0 37.0 8 33.66790412658468 37.0 35.0 37.0 28.0 37.0 9 35.25919527908309 39.0 35.0 39.0 29.0 39.0 10 35.13344313776488 39.0 35.0 39.0 28.0 39.0 11 35.148033009865706 39.0 35.0 39.0 29.0 39.0 12 35.059260124911695 39.0 35.0 39.0 28.0 39.0 13 35.0497476460437 39.0 35.0 39.0 28.0 39.0 14 36.18764607884831 40.0 36.0 41.0 28.0 41.0 15 36.133502008639084 40.0 36.0 41.0 27.0 41.0 16 36.07024745097901 40.0 36.0 41.0 27.0 41.0 17 36.04430252951452 40.0 36.0 41.0 27.0 41.0 18 35.98704181765142 40.0 36.0 41.0 27.0 41.0 19 36.01845821573803 40.0 36.0 41.0 27.0 41.0 20 35.94741732596188 40.0 36.0 41.0 27.0 41.0 21 35.859984851733266 40.0 36.0 41.0 27.0 41.0 22 35.75384945223727 40.0 36.0 41.0 27.0 41.0 23 35.6406086896925 40.0 36.0 41.0 27.0 41.0 24 35.531876821043085 40.0 36.0 41.0 27.0 41.0 25 35.423921696465285 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1054710548174072 1101 2 3.5877312343072063 1101 3 2.156272812934091 1101 4 1.3462247928503501 1101 5 0.8610574988680639 1101 6 0.6742889030562793 1101 7 0.523625082141173 1101 8 0.4227703786619088 1101 9 0.3858055767488011 1101 10 0.4832388564229788 1101 11 0.3804035742456833 1101 12 0.5544533241671559 1101 13 0.37782259800344065 1101 14 0.6594723954975876 1101 15 0.4944336222216421 1101 16 0.5366370073398627 1101 17 0.589702450146909 1101 18 0.5060738806321865 1101 19 0.42106895262516275 1101 20 0.6092271612879827 1101 21 0.6335182703176798 1101 22 0.6116104453029862 1101 23 0.6325122042670372 1101 24 0.79734491317042 1101 25 0.679466334655153 1102 1 1.5835559993612947 1102 2 4.220311223558749 1102 3 2.8426371369450436 1102 4 1.6432725642382948 1102 5 1.357677183354319 1102 6 0.990254910004694 1102 7 0.9681168684584733 1102 8 0.9995874602443919 1102 9 1.1266566936996227 1102 10 1.080456053530206 1102 11 1.097724722468591 1102 12 1.1152262714870176 1102 13 1.0441568136411803 1102 14 1.1557405864138701 1102 15 1.2699860674954948 1102 16 1.3191875864446985 1102 17 1.3053740382525163 1102 18 1.1267871930610411 1102 19 1.2531354280200873 1102 20 1.3665312981742304 1102 21 1.1613693089825006 1102 22 0.9644372576239348 1102 23 1.0839915452258495 1102 24 1.032777369277369 1102 25 0.9225184519741347 1103 1 1.482957939926802 1103 2 4.032775158782091 1103 3 2.5934933408604834 1103 4 1.7135260398058207 1103 5 1.1770750998839503 1103 6 1.0228738479945605 1103 7 0.838857871449413 1103 8 0.7390739571534937 1103 9 0.8745069944060262 1103 10 0.7847322365258194 1103 11 0.7431086245194365 1103 12 0.986170281928878 1103 13 1.00959576243919 1103 14 1.1373025056210295 1103 15 0.8503098218177882 1103 16 0.9020464833687569 1103 17 1.00862744546302 1103 18 1.0717695943528582 1103 19 1.1052670707424497 1103 20 1.286475168032787 1103 21 1.1332000260476391 1103 22 1.1754115257201647 1103 23 1.0259399266477018 1103 24 1.1979725543636732 1103 25 0.9184753277229945 1104 1 1.182450767509998 1104 2 3.7547435792988146 1104 3 2.273372754072664 1104 4 1.4963711509982787 1104 5 0.8881919216681169 1104 6 0.7366392637813917 1104 7 0.571440547583947 1104 8 0.5311996932444316 1104 9 0.5291073052067077 1104 10 0.6101951040864222 1104 11 0.7326032218725373 1104 12 0.7346016112200218 1104 13 0.48646220299552567 1104 14 0.7278659955103137 1104 15 0.6353739335508735 1104 16 0.4382002157650007 1104 17 0.574935390275293 1104 18 0.47026286559070485 1104 19 0.5322524252903946 1104 20 0.5592958660979193 1104 21 0.5770108012795845 1104 22 0.6370038822536443 1104 23 0.6554686587409009 1104 24 0.7183532730841975 1104 25 0.5150172458141711 1105 1 1.2972925487294624 1105 2 3.6554398962212815 1105 3 2.2679455780593223 1105 4 1.2702674400019447 1105 5 0.9140076281974459 1105 6 0.6663201629137063 1105 7 0.6558880308510311 1105 8 0.5256779239271978 1105 9 0.5961826822941916 1105 10 0.29883802997670017 1105 11 0.4067426064833981 1105 12 0.5963631405825183 1105 13 0.5026293925274459 1105 14 0.5117648376147983 1105 15 0.6465336474566428 1105 16 0.6139665133647227 1105 17 0.6895581374852497 1105 18 0.5605893686140178 1105 19 0.5997603601073394 1105 20 0.5823242844978722 1105 21 0.5537835532651272 1105 22 0.691940550659119 1105 23 0.6315934925138365 1105 24 0.5603369956888784 1105 25 0.46274893512200066 1106 1 0.8670622998620878 1106 2 3.567262130855841 1106 3 2.1378852230664585 1106 4 1.347524933950929 1106 5 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-1.9374756370887667 2315 18 -1.9301343475855006 2315 19 -1.9035517680774205 2315 20 -2.0101492716168323 2315 21 -2.1169410862451343 2315 22 -1.8593400614095543 2315 23 -1.9717631737897037 2315 24 -2.004907030689914 2315 25 -1.906314143607844 2316 1 -0.5590229660427788 2316 2 1.7936604783691266 2316 3 0.41480745742858005 2316 4 -0.5218882707907468 2316 5 -0.6869407210295506 2316 6 -0.8506788073618381 2316 7 -1.2016833042426427 2316 8 -1.1533387406397395 2316 9 -1.4704869946988595 2316 10 -1.5379757120239717 2316 11 -1.5343858545349107 2316 12 -1.6556031060525527 2316 13 -1.6082234661470451 2316 14 -1.861701984946066 2316 15 -1.83975485151106 2316 16 -1.8664124792279466 2316 17 -1.878470043026347 2316 18 -2.1477717111439887 2316 19 -2.0509125281861955 2316 20 -1.7991600228099571 2316 21 -2.0772072640609807 2316 22 -1.8945591960878048 2316 23 -2.0907746037686152 2316 24 -2.055983140630701 2316 25 -1.951618581378014 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 74915.0 3 0.0 4 627.0 5 3342.0 6 4088.0 7 3611.0 8 3368.0 9 3261.0 10 3206.0 11 3119.0 12 3047.0 13 2993.0 14 3038.0 15 3275.0 16 3456.0 17 3714.0 18 4254.0 19 5262.0 20 6784.0 21 8687.0 22 10882.0 23 13107.0 24 15195.0 25 17422.0 26 19915.0 27 22466.0 28 25869.0 29 31811.0 30 39408.0 31 50529.0 32 65342.0 33 86281.0 34 117525.0 35 167799.0 36 268110.0 37 341629.0 38 838831.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.784891097669416 18.812627187448378 13.10755620850482 25.294925506377385 2 16.17572245842478 21.23683413070827 40.31561355017034 22.27182986069661 3 18.76017060252143 24.624280808797945 29.787036809233765 26.828511779446863 4 12.144885614770088 17.225529925734833 38.669201921826506 31.960382537668576 5 13.081679383947053 37.79909918775767 36.066010944710584 13.05321048358469 6 31.11765036675676 37.46920262476232 18.517877415023847 12.895269593457073 7 27.5154118676653 31.535633573620224 22.609534972813957 18.33941958590051 8 24.41625574210691 37.7124623490006 21.029642803167427 16.84163910572506 9 26.271391215411168 15.43264820522914 20.494357182773857 37.80160339658584 10 14.977936602219168 29.203995487152092 34.75081804155054 21.067249869078207 11 34.229415403432434 22.338553217512942 24.992311639562633 18.439719739491988 12 23.271480839727122 24.365776164149572 32.42805451970153 19.934688476421776 13 29.130231160441582 22.007558317312252 25.934772828719144 22.92743769352702 14 20.63718495295602 22.409769401907063 27.925487046650332 29.02755859848658 15 22.692569265537518 31.345709104073162 23.647112163952748 22.314609466436572 16 21.68833759195279 28.096915517659504 28.473117977231908 21.741628913155793 17 21.377947497724247 28.43160083087013 28.34992847628119 21.840523195124437 18 21.17888486262877 27.536983210378146 30.512905901497607 20.77122602549548 19 23.35773253895694 26.774002083326927 27.590110918926424 22.27815445878971 20 23.19503657023559 27.622785312859154 28.547365572312767 20.63481254459249 21 22.811347703435743 26.86993582615641 27.38312448021032 22.93559199019753 22 22.766573643846787 28.34649301625908 27.686033924039954 21.20089941585418 23 22.277705775102696 27.78898578740417 27.963798519429737 21.969509918063395 24 23.04381750380464 26.81427732926568 28.683910853680395 21.457994313249284 25 22.66093715402378 27.383611403024734 28.415433307207554 21.540018135743935 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 288.0 1 288.0 2 468.0 3 648.0 4 648.0 5 648.0 6 1602.5 7 2557.0 8 2557.0 9 2557.0 10 3587.5 11 4618.0 12 4618.0 13 4618.0 14 7467.5 15 10317.0 16 10317.0 17 10317.0 18 18475.5 19 26634.0 20 26634.0 21 26634.0 22 43499.5 23 60365.0 24 60365.0 25 60365.0 26 87134.0 27 113903.0 28 113903.0 29 113903.0 30 150055.0 31 186207.0 32 186207.0 33 186207.0 34 206352.0 35 226497.0 36 226497.0 37 226497.0 38 240302.5 39 254108.0 40 254108.0 41 254108.0 42 267984.5 43 281861.0 44 281861.0 45 281861.0 46 288226.5 47 294592.0 48 294592.0 49 294592.0 50 282642.0 51 270692.0 52 270692.0 53 270692.0 54 240246.0 55 209800.0 56 209800.0 57 209800.0 58 179656.0 59 149512.0 60 149512.0 61 149512.0 62 122529.0 63 95546.0 64 95546.0 65 95546.0 66 73117.0 67 50688.0 68 50688.0 69 50688.0 70 36965.0 71 23242.0 72 23242.0 73 23242.0 74 16243.5 75 9245.0 76 9245.0 77 9245.0 78 6299.0 79 3353.0 80 3353.0 81 3353.0 82 2204.5 83 1056.0 84 1056.0 85 1056.0 86 681.5 87 307.0 88 307.0 89 307.0 90 198.5 91 90.0 92 90.0 93 90.0 94 61.0 95 32.0 96 32.0 97 32.0 98 21.0 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.313741340709473 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.393348821352378E-5 20 0.0 21 3.075344174946665E-4 22 0.0010544037171245706 23 7.908027878434281E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2276168.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.4970033339719 #Duplication Level Percentage of deduplicated Percentage of total 1 82.64299271033506 58.26083332629906 2 11.945079163160024 16.841845711797006 3 2.9950900495454906 6.334346196250634 4 1.0899583901460907 3.0735520105607836 5 0.4660657748520824 1.642812024179872 6 0.2373759172588118 1.0040574498239454 7 0.14661422806786803 0.7235104607435746 8 0.09098908329646237 0.5131566166804603 9 0.060721620448274415 0.385262305126756 >10 0.280888589939215 3.450771337201682 >50 0.021609203323170952 1.06399367053678 >100 0.018386366907335496 2.649935429775977 >500 0.0023984821397747555 1.223825246533566 >1k 0.001704184678259937 2.3539858572329817 >5k 1.2623590209332868E-4 0.47811235725702483 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5504 0.2418099191272349 No Hit GTCCTACAGTGGACATTTCTAAATT 5241 0.2302554117270781 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4669 0.2051254564689425 No Hit CTGTAGGACGTGGAATATGGCAAGA 4597 0.20196224531756884 No Hit CTTTAGGACGTGAAATATGGCGAGG 4040 0.17749129238263606 No Hit TATCAACGCAGAGTACTTTTTTTTT 3424 0.15042826364310544 No Hit GGTATCAACGCAGAGTACTTTTTTT 3405 0.14959352736704848 No Hit CTGAAGGACCTGGAATATGGCGAGA 2341 0.10284829590785918 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 8.786697642704756E-5 0.0 4.393348821352378E-5 0.0 6 0.0 8.786697642704756E-5 0.0 4.393348821352378E-5 0.0 7 0.0 8.786697642704756E-5 0.0 4.393348821352378E-5 0.0 8 0.0 8.786697642704756E-5 0.0 4.393348821352378E-5 0.0 9 0.0 8.786697642704756E-5 0.0 1.3180046464057136E-4 4.393348821352378E-5 10 0.0 2.1966744106761892E-4 0.0 1.3180046464057136E-4 4.393348821352378E-5 11 0.0 2.1966744106761892E-4 0.0 1.3180046464057136E-4 4.393348821352378E-5 12 0.0 2.1966744106761892E-4 0.0 1.3180046464057136E-4 1.3180046464057136E-4 13 0.0 2.1966744106761892E-4 0.0 1.3180046464057136E-4 1.7573395285409513E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 380 0.0 14.434434 18 CCAACGA 220 0.0 13.325832 19 GGTATCA 1010 0.0 12.622843 1 GGCGAGG 1085 0.0 11.85399 19 TACACCG 80 3.0045068E-5 11.821044 5 GACCGTC 65 8.338412E-4 11.639181 7 ACCGTCT 65 8.338412E-4 11.639181 8 CGTTAGG 80 2.6164638E-4 11.118376 1 CGTTTAC 60 0.0060796617 11.032975 14 TAGGACC 695 0.0 11.021635 4 CCGAATG 70 0.0015493322 10.808049 18 CCAACGT 210 0.0 10.808049 19 TGTGCGA 70 0.001549616 10.807812 10 GTATCAA 2095 0.0 10.7085495 1 TGTAGGA 2220 0.0 10.639808 2 TATCGCG 80 3.934206E-4 10.638939 7 CGGTAGG 75 0.001906989 10.541866 1 AGGACGT 2595 0.0 10.459004 5 GTATTAG 180 1.9099389E-10 10.432056 1 GTCCTAA 1450 0.0 10.428269 1 >>END_MODULE