FastQCFastQC Report
Thu 26 May 2016
SRR1511755_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511755_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276168
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74850.3288421592782255No Hit
GTCCTACAGTGGACATTTCTAAATT57840.2541112958270216No Hit
CTGTAGGACGTGGAATATGGCAAGA52240.22950854242744823No Hit
GTCCTAAAGTGTGTATTTCTCATTT51350.22559846197644462No Hit
TATCAACGCAGAGTACTTTTTTTTT48030.2110125438895547No Hit
CTTTAGGACGTGAAATATGGCGAGG45050.19792036440192465No Hit
GGTATCAACGCAGAGTACTTTTTTT43930.19299981372200997No Hit
CTGAAGGACCTGGAATATGGCGAGA26780.11765388143581668No Hit
TTGTAGAACAGTGTATATCAATGAG25860.1136120005201725No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23240.10210142660822927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG400.005277304414.2511385
CCAACGA2250.013.92924119
TCCAACG3350.013.32415218
GGCGAGG10800.012.5750119
GGTATCA13800.011.9581981
CGCCCTA1408.398274E-910.85371516
TGGCGAG24950.010.39150918
CCCGTTT2750.010.36036512
CGACTAA1106.070868E-610.36036517
ACGAAAT2200.010.36036512
GTGTTAC1752.1827873E-1010.3565311
TAGGACC5600.010.349044
GTATCAA28200.010.3169061
GCCTTAT1951.4551915E-1110.2726721
AAGCGTG750.002646625710.1341435
CTTATCC1502.4430847E-810.1341433
GACGTGA13250.010.1062437
TTAGGAC15950.010.0666343
TCCCGTT2750.010.01501911
TGTAGGA23450.010.0071952
Produced by FastQC (version 0.11.5)