FastQCFastQC Report
Thu 26 May 2016
SRR1511754_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511754_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753856
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27930.3704951608795314No Hit
GGTATCAACGCAGAGTACTTTTTTT16460.2183440869343747No Hit
TATCAACGCAGAGTACTTTTTTTTT16220.21516045504711775No Hit
GTCCTACAGTGGACATTTCTAAATT12720.1687324900246201No Hit
CTGTAGGACGTGGAATATGGCAAGA11230.14896744205789966No Hit
GTCCTAAAGTGTGTATTTCTCATTT10730.14233487562611427No Hit
CTTTAGGACGTGAAATATGGCGAGG8820.11699847185669413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4400.013.2406071
GTATAAC1008.688221E-812.8366571
GGACGTG5850.012.44875056
TCCAACG1155.667971E-912.33628318
GGCGAGG2800.012.1600519
GACGTGA3000.011.9799017
TAGGACC2500.011.7276934
AGGACGT6250.011.652035
TAGACTA658.3198294E-411.640395
GTCCTAT853.5822773E-511.6168831
CCCTTAG600.00440116211.5200771
ACGTGGA3400.011.4050148
ACGTGAA3400.011.12684258
GACGTGG3500.011.0791567
ATTGGAC600.006068588711.034123
CGTGGAA3350.011.01059159
CGTGAAA3400.010.8486729
GTCCTAA3650.010.8212071
GTAGGAC7350.010.8089333
AGGACCT4500.010.7188595
Produced by FastQC (version 0.11.5)