##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511754_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 753856 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.078829378555056 31.0 30.0 34.0 25.0 34.0 2 27.46874602046014 31.0 27.0 34.0 10.0 34.0 3 29.135657207742593 31.0 28.0 34.0 16.0 34.0 4 33.64521606248408 35.0 33.0 37.0 28.0 37.0 5 34.0827532048561 37.0 35.0 37.0 30.0 37.0 6 34.33808976780711 37.0 35.0 37.0 31.0 37.0 7 34.40486246710247 37.0 35.0 37.0 32.0 37.0 8 34.42800747092283 37.0 35.0 37.0 32.0 37.0 9 36.10514872866967 39.0 37.0 39.0 32.0 39.0 10 35.99470986501401 39.0 37.0 39.0 32.0 39.0 11 36.01628295059003 39.0 37.0 39.0 32.0 39.0 12 35.93999915103149 39.0 37.0 39.0 32.0 39.0 13 35.94392032430597 39.0 37.0 39.0 32.0 39.0 14 37.17551627897105 40.0 38.0 41.0 32.0 41.0 15 37.12662763180236 40.0 38.0 41.0 32.0 41.0 16 37.07313333050344 40.0 38.0 41.0 31.0 41.0 17 37.057785571780286 40.0 38.0 41.0 31.0 41.0 18 37.008770905849396 40.0 38.0 41.0 31.0 41.0 19 37.05319052975634 40.0 38.0 41.0 31.0 41.0 20 36.988979327616946 40.0 38.0 41.0 31.0 41.0 21 36.92278366160115 40.0 38.0 41.0 31.0 41.0 22 36.83387278206978 40.0 38.0 41.0 31.0 41.0 23 36.75512299431191 40.0 38.0 41.0 31.0 41.0 24 36.66430856821462 40.0 37.0 41.0 31.0 41.0 25 36.571186008999064 40.0 37.0 41.0 31.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7024836672861987 1101 2 3.387648203098177 1101 3 1.8337592123740656 1101 4 0.8030012880343094 1101 5 0.4952396370230332 1101 6 0.20220999067102952 1101 7 0.31275096169276395 1101 8 0.2590889233472282 1101 9 -0.11901805491494599 1101 10 0.3647245541194195 1101 11 0.23838780666926596 1101 12 0.1647412492240221 1101 13 0.14252475829266587 1101 14 0.13281945999516154 1101 15 0.19500832619193176 1101 16 0.10959051681540188 1101 17 0.06877876692144014 1101 18 0.018178456080235605 1101 19 -0.005061559334741617 1101 20 0.22787554316323622 1101 21 0.35430044501567437 1101 22 0.2984667339470448 1101 23 0.16440715100774383 1101 24 0.12252836091256825 1101 25 -0.12845065407051948 1102 1 1.5236120090354106 1102 2 4.169617912234717 1102 3 2.750504212051286 1102 4 1.6609122922871222 1102 5 1.2322186437762 1102 6 0.7603614260939224 1102 7 0.8319862425658044 1102 8 0.891717338312219 1102 9 0.9166230712539516 1102 10 0.9242600039398496 1102 11 0.9364034228863218 1102 12 0.8058170330658498 1102 13 0.8989681404522614 1102 14 0.9753866413766232 1102 15 0.9991301383533084 1102 16 1.0496401775822974 1102 17 1.105786587626234 1102 18 1.0390236968184396 1102 19 1.0081283025629801 1102 20 0.9518394611678644 1102 21 1.0011012303926776 1102 22 0.8437180215213047 1102 23 0.6169294739475859 1102 24 0.5763197573500349 1102 25 0.6781400256395784 1103 1 1.5312386719612228 1103 2 4.174088230908588 1103 3 2.776306741802756 1103 4 1.686691441710792 1103 5 1.2052985343562668 1103 6 1.0534946238213578 1103 7 0.913265614102599 1103 8 0.720236662153134 1103 9 1.0096674237357703 1103 10 1.0117912751282674 1103 11 1.2121229524741253 1103 12 1.2406396932795474 1103 13 1.1643862976148256 1103 14 1.2065058576680343 1103 15 0.9215121261487766 1103 16 1.145625775300374 1103 17 1.2375160304730244 1103 18 1.1023656808730564 1103 19 1.1821808147792012 1103 20 1.080071206976939 1103 21 1.2247247135939077 1103 22 1.400614049203611 1103 23 1.111418730686971 1103 24 1.2949608926987537 1103 25 0.9278606307224635 1104 1 1.2687576215145953 1104 2 3.9146624315289635 1104 3 2.3713297144920737 1104 4 1.527930334357201 1104 5 0.902676304878149 1104 6 0.9332001380442136 1104 7 0.6917684633380077 1104 8 0.7562264313378577 1104 9 0.7176180732681559 1104 10 0.5994583227295465 1104 11 0.8005985143526786 1104 12 0.5827671742133802 1104 13 0.7794775275671739 1104 14 0.9181678987001405 1104 15 0.8567128164955804 1104 16 0.860248222455418 1104 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16 -2.1054414768671066 2315 17 -2.0622523428319965 2315 18 -2.0086188934324625 2315 19 -2.0407534450609077 2315 20 -1.8987992911813052 2315 21 -2.168387609267967 2315 22 -2.038495474835699 2315 23 -2.065191798676544 2315 24 -2.0543289462885284 2315 25 -2.0325167277681047 2316 1 -0.8883643665097409 2316 2 1.84791817454985 2316 3 0.25234202727200383 2316 4 -0.9575624176374049 2316 5 -1.0014236742527345 2316 6 -1.05495101858655 2316 7 -1.577014545988881 2316 8 -1.49097790943879 2316 9 -1.5510284841299438 2316 10 -1.719059006665411 2316 11 -1.6577210400113742 2316 12 -1.6594093143166262 2316 13 -1.7726471092299008 2316 14 -1.7410233426913493 2316 15 -1.8788064232263793 2316 16 -2.1204446322794652 2316 17 -2.149643979328495 2316 18 -2.3569498463670016 2316 19 -2.1773960863071338 2316 20 -1.7082323134020498 2316 21 -2.2115735793081583 2316 22 -1.8240204358082295 2316 23 -1.9159275079800224 2316 24 -1.9533524291994766 2316 25 -2.200049195300572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19528.0 3 0.0 4 177.0 5 836.0 6 1066.0 7 934.0 8 788.0 9 759.0 10 711.0 11 733.0 12 754.0 13 675.0 14 738.0 15 716.0 16 765.0 17 808.0 18 947.0 19 1132.0 20 1496.0 21 1826.0 22 2218.0 23 2689.0 24 3163.0 25 3588.0 26 4133.0 27 4859.0 28 5677.0 29 7030.0 30 9066.0 31 11944.0 32 16951.0 33 24016.0 34 35234.0 35 54628.0 36 92865.0 37 127561.0 38 312845.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.640122251464476 19.111076916546395 13.514119407419987 26.73468142456915 2 15.039840223598322 21.123420899185465 41.198378413565486 22.63836046365073 3 17.251835894388318 24.69954474064012 30.69565009763138 27.352969267340182 4 11.129181169878597 17.308478011715767 39.620431488241785 31.941909330163853 5 12.112923423041005 37.56274407844469 37.05442418711266 13.269908311401647 6 29.38359580609559 38.40043721877918 19.080434459631547 13.135532515493676 7 25.58804333984209 32.07111172425503 23.78053633585194 18.560308600050938 8 22.862854444350113 37.80523070719076 21.962682528228203 17.36923232023092 9 24.717983275320485 15.599530944901943 21.291068851345614 38.39141692843195 10 13.397253586891928 29.33849435435945 35.68294210034807 21.581309958400542 11 32.234140207148315 23.04869895576874 25.904151455980983 18.81300938110196 12 21.52758617030308 24.974530944901943 33.35477863146277 20.1431042533322 13 27.2831946684778 22.40812568978691 27.132900713133544 23.175778928601748 14 19.319737456490362 22.632571737838525 29.03512076576959 29.01257003990152 15 21.360843450207998 31.392865480940657 24.67832052805841 22.567970540792935 16 20.361978945581118 28.73267573648018 29.183026997198404 21.7223183207403 17 19.986973639527974 28.851929280923677 29.41622803293998 21.74486904660837 18 19.68545716953901 27.99181806604975 31.49872124119195 20.82400352321929 19 21.74572264656021 27.20075770639938 29.104044019133678 21.949475627906732 20 21.411250955089567 27.733280626538754 29.953200611257323 20.902267807114356 21 21.11008379617777 27.14441674968462 28.977400103203145 22.768099350934463 22 20.943102890222484 28.477207727776804 29.33772146256825 21.24196791943246 23 20.627816372200872 27.94464688645369 29.520157166336585 21.907379575008854 24 21.03399057644961 27.133298667119448 30.385113337295188 21.44759741913575 25 20.83209525426607 27.57237456490364 29.927864207487904 21.66766597334239 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 159.0 1 159.0 2 247.0 3 335.0 4 335.0 5 335.0 6 779.5 7 1224.0 8 1224.0 9 1224.0 10 1696.0 11 2168.0 12 2168.0 13 2168.0 14 3448.0 15 4728.0 16 4728.0 17 4728.0 18 8384.0 19 12040.0 20 12040.0 21 12040.0 22 18354.0 23 24668.0 24 24668.0 25 24668.0 26 34617.5 27 44567.0 28 44567.0 29 44567.0 30 56602.5 31 68638.0 32 68638.0 33 68638.0 34 75275.5 35 81913.0 36 81913.0 37 81913.0 38 85731.0 39 89549.0 40 89549.0 41 89549.0 42 91592.5 43 93636.0 44 93636.0 45 93636.0 46 93211.5 47 92787.0 48 92787.0 49 92787.0 50 87864.0 51 82941.0 52 82941.0 53 82941.0 54 72117.0 55 61293.0 56 61293.0 57 61293.0 58 51929.5 59 42566.0 60 42566.0 61 42566.0 62 34560.5 63 26555.0 64 26555.0 65 26555.0 66 20149.0 67 13743.0 68 13743.0 69 13743.0 70 10122.0 71 6501.0 72 6501.0 73 6501.0 74 4519.0 75 2537.0 76 2537.0 77 2537.0 78 1729.0 79 921.0 80 921.0 81 921.0 82 601.5 83 282.0 84 282.0 85 282.0 86 176.5 87 71.0 88 71.0 89 71.0 90 44.5 91 18.0 92 18.0 93 18.0 94 15.0 95 12.0 96 12.0 97 12.0 98 8.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.224546863061381 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 2.653026572714152E-4 20 0.0 21 3.979539859071228E-4 22 5.306053145428304E-4 23 6.632566431785381E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 753856.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.1010827587761 #Duplication Level Percentage of deduplicated Percentage of total 1 89.33525325161744 71.5585051398403 2 7.7640003137474265 12.438096633412925 3 1.5747167369945525 3.7840954701489156 4 0.5383828415202808 1.7250019417808418 5 0.2331853404951757 0.9339199128568726 6 0.12803840754247575 0.6153609047317051 7 0.08397680471711573 0.47086430891242864 8 0.05981266737441535 0.3832847535504953 9 0.04561990959962928 0.328878373885901 >10 0.20440016666926855 2.9011868141377817 >50 0.018732298181341627 1.065327893444663 >100 0.012041576293024776 2.0363920297357976 >500 8.362205671847185E-4 0.48253957325933416 >1k 0.0010034646806216622 1.2765462503021086 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2793 0.3704951608795314 No Hit GGTATCAACGCAGAGTACTTTTTTT 1646 0.2183440869343747 No Hit TATCAACGCAGAGTACTTTTTTTTT 1622 0.21516045504711775 No Hit GTCCTACAGTGGACATTTCTAAATT 1272 0.1687324900246201 No Hit CTGTAGGACGTGGAATATGGCAAGA 1123 0.14896744205789966 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1073 0.14233487562611427 No Hit CTTTAGGACGTGAAATATGGCGAGG 882 0.11699847185669413 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.653026572714152E-4 0.0 0.0 0.0 0.0 7 2.653026572714152E-4 0.0 0.0 0.0 0.0 8 2.653026572714152E-4 0.0 0.0 0.0 0.0 9 2.653026572714152E-4 0.0 0.0 0.0 0.0 10 2.653026572714152E-4 0.0 0.0 0.0 0.0 11 2.653026572714152E-4 0.0 0.0 0.0 0.0 12 2.653026572714152E-4 0.0 0.0 0.0 0.0 13 2.653026572714152E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 440 0.0 13.240607 1 GTATAAC 100 8.688221E-8 12.836657 1 GGACGTG 585 0.0 12.4487505 6 TCCAACG 115 5.667971E-9 12.336283 18 GGCGAGG 280 0.0 12.16005 19 GACGTGA 300 0.0 11.979901 7 TAGGACC 250 0.0 11.727693 4 AGGACGT 625 0.0 11.65203 5 TAGACTA 65 8.3198294E-4 11.64039 5 GTCCTAT 85 3.5822773E-5 11.616883 1 CCCTTAG 60 0.004401162 11.520077 1 ACGTGGA 340 0.0 11.405014 8 ACGTGAA 340 0.0 11.1268425 8 GACGTGG 350 0.0 11.079156 7 ATTGGAC 60 0.0060685887 11.03412 3 CGTGGAA 335 0.0 11.0105915 9 CGTGAAA 340 0.0 10.848672 9 GTCCTAA 365 0.0 10.821207 1 GTAGGAC 735 0.0 10.808933 3 AGGACCT 450 0.0 10.718859 5 >>END_MODULE