FastQCFastQC Report
Thu 26 May 2016
SRR1511754_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511754_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753856
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44050.584329102640292No Hit
TATCAACGCAGAGTACTTTTTTTTT32220.4274025808642499No Hit
GGTATCAACGCAGAGTACTTTTTTT30720.40750488156889375No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16210.21502780371848207No Hit
CTGTAGGACGTGGAATATGGCAAGA10120.13424314457933612No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10100.1339778419220647No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10040.13318193395025044No Hit
GTCCTACAGTGGACATTTCTAAATT9160.12150861703030817No Hit
CTTTAGGACGTGAAATATGGCGAGG8840.11726377451396552No Hit
GTCCTAAAGTGTGTATTTCTCATTT8170.10837613549537313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC456.7526806E-414.7789133
GGTATCA6900.013.3978781
ACAGCTC802.8692493E-511.8719748
GGACTGC700.001493030110.8543766
AGTGTAT1605.4387783E-1010.68477610
TAATACT909.491851E-510.5563664
TCAAGAC909.491851E-510.5563663
GAACAGT1553.6598067E-910.41676
GTATCAA15250.010.3741281
GTGGTAT2053.6379788E-1210.2277991
TCTATAC750.00264318110.1341113
TGCAATT750.002650465110.13075112
CTAGACA856.579905E-410.0595964
TAGACTG856.579905E-410.0595965
ACCAGGC856.600004E-410.056267
ATAGGAC951.6400013E-410.0007693
AGTACTG951.6400013E-410.0007695
TTATTCC1054.0931336E-59.9531464
ACTGTCC1054.108082E-59.9498448
AGGACTG1252.5599365E-69.8807595
Produced by FastQC (version 0.11.5)