##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511754_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 753856 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.25029448594957 31.0 28.0 31.0 22.0 34.0 2 28.42797032855081 31.0 28.0 31.0 22.0 34.0 3 28.406029798794464 31.0 28.0 31.0 22.0 34.0 4 31.88450048815689 35.0 32.0 37.0 24.0 37.0 5 31.305058260463536 35.0 32.0 37.0 17.0 37.0 6 31.31829686306138 35.0 32.0 37.0 17.0 37.0 7 31.0311916334154 35.0 32.0 37.0 17.0 37.0 8 30.85681880889719 35.0 31.0 37.0 13.0 37.0 9 31.880726557857205 37.0 31.0 39.0 11.0 39.0 10 31.487475061550217 35.0 30.0 39.0 10.0 39.0 11 31.60128326895322 37.0 31.0 39.0 10.0 39.0 12 31.22939394260973 35.0 30.0 39.0 10.0 39.0 13 31.213726494184566 35.0 30.0 39.0 10.0 39.0 14 31.657658757110113 37.0 30.0 40.0 8.0 41.0 15 31.550304036845233 36.0 30.0 40.0 2.0 41.0 16 31.294106566771372 36.0 30.0 39.0 2.0 41.0 17 31.218463473130146 36.0 30.0 39.0 2.0 40.0 18 31.068235843450207 36.0 30.0 39.0 2.0 40.0 19 31.10019950759827 36.0 30.0 39.0 2.0 40.0 20 31.1763281051023 37.0 30.0 40.0 2.0 41.0 21 31.05748180023771 37.0 30.0 40.0 2.0 41.0 22 30.826006558281687 36.0 30.0 40.0 2.0 41.0 23 30.601533979964344 36.0 29.0 39.0 2.0 41.0 24 30.26811619195178 36.0 29.0 39.0 2.0 41.0 25 29.571943448085577 36.0 27.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.605754417079293 1101 2 0.6292310314837763 1101 3 0.5387618902229327 1101 4 0.7977434262411798 1101 5 0.926662732339981 1101 6 1.0053802727238903 1101 7 0.8895701256304918 1101 8 1.0202934105092432 1101 9 0.8229067006946558 1101 10 1.1257242057942882 1101 11 0.8574503680190517 1101 12 1.1298495792424958 1101 13 1.127674784422009 1101 14 0.757161605037755 1101 15 0.7958832988734166 1101 16 0.5241836876522079 1101 17 0.39877680647949276 1101 18 0.6030662549260875 1101 19 0.6618694878931279 1101 20 0.45494277914092507 1101 21 0.40713012182852637 1101 22 0.49823232456499866 1101 23 0.44871032362865293 1101 24 -0.25261147628594927 1101 25 5.086501979434388E-4 1102 1 0.38028918197791484 1102 2 0.47050394684161034 1102 3 0.44148724917706517 1102 4 0.6058598283075973 1102 5 0.4345204386549639 1102 6 0.5706076588890987 1102 7 0.42817216087168575 1102 8 0.40204677882869433 1102 9 0.5770670101848374 1102 10 0.5351562875314393 1102 11 0.694075262328532 1102 12 0.7469641217855845 1102 13 0.482276907837047 1102 14 0.43716928875818795 1102 15 0.6722336576307306 1102 16 0.37232156634704694 1102 17 0.590411628218753 1102 18 0.35064186793109897 1102 19 0.5297598815105999 1102 20 0.7743052822703085 1102 21 0.4507257880842417 1102 22 0.9994643965400449 1102 23 1.1586215357198064 1102 24 0.240058285912351 1102 25 0.43715968175315467 1103 1 -0.0027741085694188428 1103 2 0.1959768139712068 1103 3 0.15083704226116268 1103 4 0.17945408609908853 1103 5 0.25111111925630425 1103 6 0.2547581346787702 1103 7 0.19228803377622228 1103 8 0.26334999829983374 1103 9 0.14195443390651974 1103 10 0.5626609051461777 1103 11 0.32721453894118824 1103 12 0.3879685406675364 1103 13 0.12046165918726004 1103 14 0.6549056054213445 1103 15 0.5811485766178954 1103 16 0.5050376590266659 1103 17 0.8240091592561392 1103 18 0.565226516407872 1103 19 0.712378945829883 1103 20 0.48272921437383687 1103 21 0.597661796127074 1103 22 0.7163426551732321 1103 23 0.9857660962865431 1103 24 0.6068021076607302 1103 25 0.3310129935765893 1104 1 -0.10456139157273725 1104 2 -0.050870369056212894 1104 3 0.08097363452896644 1104 4 0.1781697922663419 1104 5 0.08281265310117902 1104 6 0.3582261944777301 1104 7 0.2663526060799448 1104 8 0.31001215594456966 1104 9 0.3770081923317825 1104 10 0.30897866680550123 1104 11 0.25457264079843966 1104 12 -0.10709408031807044 1104 13 0.3927925903002887 1104 14 0.19219599420787148 1104 15 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1107 14 1.0951473889418857 1107 15 0.778280456313464 1107 16 0.9826041617308228 1107 17 1.0873378429063827 1107 18 0.8211793343605436 1107 19 0.7898720324852917 1107 20 0.43395648598874814 1107 21 0.5628430114074021 1107 22 0.9109490227918613 1107 23 0.6939519857219736 1107 24 0.7104655684932695 1107 25 0.6418810159929542 1108 1 0.15071922737088173 1108 2 -0.019966419319761286 1108 3 0.034714579980718696 1108 4 0.12726097500970113 1108 5 -0.1175482479024268 1108 6 0.2516835428320583 1108 7 0.09262856885622384 1108 8 0.4603112522510173 1108 9 0.4900627533903368 1108 10 0.8170340924893544 1108 11 0.4100835062563384 1108 12 0.48637992007901687 1108 13 0.3920880807990166 1108 14 0.4206011768359481 1108 15 0.6224233835393953 1108 16 0.34240201526872127 1108 17 0.4055941952648716 1108 18 0.5635971619581035 1108 19 0.6307287356431566 1108 20 0.4798208166810056 1108 21 0.691603856282839 1108 22 0.4151744080327511 1108 23 0.42160700611806945 1108 24 0.992934671938766 1108 25 0.7737819185285204 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-0.05025110974967362 2314 2 0.06302504277914878 2314 3 0.004475625307517106 2314 4 -0.14540242390075164 2314 5 -0.04593177414881211 2314 6 -0.04206487545849669 2314 7 -0.4198686007163488 2314 8 -0.3902255299858872 2314 9 -0.27731607101660316 2314 10 -1.2647452806875172 2314 11 -0.4472030129173099 2314 12 -0.246570917061522 2314 13 -0.6318673249843769 2314 14 -0.20722758563458044 2314 15 -0.3814259977624701 2314 16 -0.11463542629127588 2314 17 -0.4398378278580566 2314 18 -0.5926690713756742 2314 19 -0.18957575174978558 2314 20 0.16384188838072689 2314 21 -0.22273076017759408 2314 22 -0.19209213165317252 2314 23 -0.1129275384162085 2314 24 -0.3236436409932928 2314 25 -0.6642983190941862 2315 1 -0.7711795839671254 2315 2 -0.962491232804517 2315 3 -1.0410013337783433 2315 4 -1.113429827105243 2315 5 -1.402890071047544 2315 6 -1.6752906034382988 2315 7 -1.0853825094205476 2315 8 -1.586827743018187 2315 9 -1.4707657320926124 2315 10 -1.5035634188738314 2315 11 -1.3673438422455781 2315 12 -1.6390159692972368 2315 13 -1.4353571572646686 2315 14 -1.787520684208534 2315 15 -1.7249654030182562 2315 16 -2.1933506052207896 2315 17 -1.9591673435024717 2315 18 -1.7522066094265476 2315 19 -1.812998653938493 2315 20 -1.772590961276407 2315 21 -1.9148589135513845 2315 22 -2.1851098372265163 2315 23 -1.7660909385700556 2315 24 -1.7131563925564173 2315 25 -1.7697526613318075 2316 1 -1.7015080790015027 2316 2 -1.8306016390223405 2316 3 -1.664191953899909 2316 4 -2.1999545555737043 2316 5 -1.9754346620064567 2316 6 -2.0623700201852344 2316 7 -2.4174481419976885 2316 8 -3.1064776583202764 2316 9 -2.601270265921954 2316 10 -2.6355094779298653 2316 11 -2.566553328411583 2316 12 -2.5221760317411857 2316 13 -2.247895769547526 2316 14 -2.1194634217778727 2316 15 -2.686236868126038 2316 16 -2.718387971946136 2316 17 -2.575900993484204 2316 18 -2.4958941239580525 2316 19 -2.3168050776937648 2316 20 -2.183455544263751 2316 21 -2.2441743551430413 2316 22 -2.5400405842288762 2316 23 -2.7459826581037206 2316 24 -2.6363735845715652 2316 25 -2.492959888886862 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39438.0 3 4.0 4 595.0 5 4341.0 6 5268.0 7 4519.0 8 3828.0 9 3384.0 10 3083.0 11 2967.0 12 2956.0 13 2713.0 14 2576.0 15 2653.0 16 2611.0 17 2823.0 18 2977.0 19 3463.0 20 4142.0 21 5237.0 22 6647.0 23 8297.0 24 9715.0 25 11261.0 26 12800.0 27 14716.0 28 16942.0 29 20481.0 30 24829.0 31 30925.0 32 38000.0 33 47913.0 34 59566.0 35 74001.0 36 92662.0 37 107928.0 38 77595.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.1291353173272 23.89432011203229 15.462346181903378 19.51419838873713 2 17.903684523304186 25.31703667543934 38.2281231428814 18.551155658375073 3 21.410057093131847 28.31721177519314 30.713425375668564 19.559305756006452 4 16.625854274556414 21.24490618898039 37.34347143220986 24.785768104253332 5 13.285893035086623 40.34212212847095 35.325595867572304 11.046388968870124 6 30.2183706172001 40.83830333644622 17.719299176500552 11.224026869853128 7 26.857251252228544 33.98089290262331 23.031852237032005 16.13000360811614 8 25.0129998302063 39.34080248747772 20.628740767467526 15.01745691484846 9 27.28160285253417 16.09286123609814 21.080418541472113 35.54511736989558 10 15.151169454113253 31.881950929620512 34.34873503693013 18.618144579336107 11 34.84936115120129 23.69033343237966 24.77409478733339 16.68621062908566 12 23.15097313014687 26.961515196536208 32.2314871805756 17.656024492741317 13 29.9000074284744 23.38589863316071 26.33686008999066 20.377233848374228 14 21.330068341964513 23.918493717633076 28.7338695984379 26.017568341964513 15 23.480081076492063 32.71513392478139 23.93719755497071 19.867587443755834 16 21.999957551574838 30.222482808387806 28.230988411579933 19.546571228457424 17 21.536075855335767 30.300083835639697 28.50889294507174 19.654947363952797 18 21.31162980728415 28.905122463706594 30.86796417352916 18.91528355548009 19 23.396643390780202 28.096877918329234 29.190057517616097 19.316421173274474 20 23.16211584175227 28.515790814160795 29.781815094659986 18.540278249426947 21 23.038750106121064 27.72014814500382 29.062977544783088 20.17812420409203 22 22.770794422276932 28.974366457254437 29.373646956447914 18.881192164020717 23 22.51570591731047 28.67165612530775 29.379350963579252 19.43328699380253 24 22.695050513625944 28.207509126411413 29.98636344341625 19.111076916546395 25 22.7503001280104 28.484635435666483 29.62346370938323 19.14160072693989 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 198.0 1 198.0 2 303.5 3 409.0 4 409.0 5 409.0 6 968.0 7 1527.0 8 1527.0 9 1527.0 10 2240.5 11 2954.0 12 2954.0 13 2954.0 14 4233.5 15 5513.0 16 5513.0 17 5513.0 18 9765.0 19 14017.0 20 14017.0 21 14017.0 22 21626.5 23 29236.0 24 29236.0 25 29236.0 26 40202.5 27 51169.0 28 51169.0 29 51169.0 30 62858.0 31 74547.0 32 74547.0 33 74547.0 34 80824.0 35 87101.0 36 87101.0 37 87101.0 38 89227.5 39 91354.0 40 91354.0 41 91354.0 42 91959.0 43 92564.0 44 92564.0 45 92564.0 46 90180.0 47 87796.0 48 87796.0 49 87796.0 50 81878.0 51 75960.0 52 75960.0 53 75960.0 54 65999.0 55 56038.0 56 56038.0 57 56038.0 58 47201.0 59 38364.0 60 38364.0 61 38364.0 62 30578.5 63 22793.0 64 22793.0 65 22793.0 66 17749.0 67 12705.0 68 12705.0 69 12705.0 70 9319.5 71 5934.0 72 5934.0 73 5934.0 74 4120.0 75 2306.0 76 2306.0 77 2306.0 78 1619.5 79 933.0 80 933.0 81 933.0 82 625.0 83 317.0 84 317.0 85 317.0 86 205.0 87 93.0 88 93.0 89 93.0 90 58.5 91 24.0 92 24.0 93 24.0 94 14.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3507301129128109 2 0.0 3 0.0 4 0.0 5 0.027458825027591477 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0014591646149927837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 753856.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.58627941021781 #Duplication Level Percentage of deduplicated Percentage of total 1 90.82099609785644 73.1892616785616 2 6.615914885151628 10.663039309780963 3 1.3454441692376626 3.2527301925910397 4 0.46823038686835255 1.5093177913810976 5 0.2272685876148755 0.9157364951348964 6 0.12982639828102 0.6277335844017901 7 0.0733894088568831 0.4139925585524046 8 0.05203828510850253 0.3354857427025889 9 0.041237117650939036 0.2990831296581538 >10 0.19194269530476032 2.801193910173054 >50 0.01825425885816346 1.04601875871296 >100 0.013155497213408448 2.278135355450814 >500 0.0011511059986920368 0.6346338306179165 >1k 0.0011511059986920368 2.033637662280757 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4405 0.584329102640292 No Hit TATCAACGCAGAGTACTTTTTTTTT 3222 0.4274025808642499 No Hit GGTATCAACGCAGAGTACTTTTTTT 3072 0.40750488156889375 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1621 0.21502780371848207 No Hit CTGTAGGACGTGGAATATGGCAAGA 1012 0.13424314457933612 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1010 0.1339778419220647 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1004 0.13318193395025044 No Hit GTCCTACAGTGGACATTTCTAAATT 916 0.12150861703030817 No Hit CTTTAGGACGTGAAATATGGCGAGG 884 0.11726377451396552 No Hit GTCCTAAAGTGTGTATTTCTCATTT 817 0.10837613549537313 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGTAC 45 6.7526806E-4 14.778913 3 GGTATCA 690 0.0 13.397878 1 ACAGCTC 80 2.8692493E-5 11.871974 8 GGACTGC 70 0.0014930301 10.854376 6 AGTGTAT 160 5.4387783E-10 10.684776 10 TAATACT 90 9.491851E-5 10.556366 4 TCAAGAC 90 9.491851E-5 10.556366 3 GAACAGT 155 3.6598067E-9 10.4167 6 GTATCAA 1525 0.0 10.374128 1 GTGGTAT 205 3.6379788E-12 10.227799 1 TCTATAC 75 0.002643181 10.134111 3 TGCAATT 75 0.0026504651 10.130751 12 CTAGACA 85 6.579905E-4 10.059596 4 TAGACTG 85 6.579905E-4 10.059596 5 ACCAGGC 85 6.600004E-4 10.05626 7 ATAGGAC 95 1.6400013E-4 10.000769 3 AGTACTG 95 1.6400013E-4 10.000769 5 TTATTCC 105 4.0931336E-5 9.953146 4 ACTGTCC 105 4.108082E-5 9.949844 8 AGGACTG 125 2.5599365E-6 9.880759 5 >>END_MODULE