FastQCFastQC Report
Thu 26 May 2016
SRR1511753_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511753_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429188
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55690.2292535612723264No Hit
TATCAACGCAGAGTACTTTTTTTTT34130.14049962374258393No Hit
GGTATCAACGCAGAGTACTTTTTTT33150.13646535385486838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGT550.003177005612.0353457
GTCCTAA5600.011.124031
AAACGGG600.00608221811.03239919
ATAGGAC1553.4015102E-1010.9815593
GCGCCAC1658.731149E-1110.88912213
TTAGACT2600.010.5474594
TAGGACT2250.010.5070484
TCCAACG1752.564775E-1010.26688618
GTACTAG1751.2878445E-910.1705421
GCGATAA750.002751619310.08676511
ATACCAT2850.09.9540446
GGTATCA14150.09.9229671
AGCGTAC907.7191764E-49.8880271
CCTAGTA1406.130522E-79.8880272
CGCCCTA1554.3661203E-89.76138616
GTATCAA27150.09.7605561
TAGGACC3700.09.711924
GCGTACA1157.177204E-59.4581132
GCCGGTT1107.1922695E-59.45634311
CTAGTAC1502.8328031E-79.4563433
Produced by FastQC (version 0.11.5)