##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511753_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2429188 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.105976565008554 31.0 28.0 34.0 22.0 34.0 2 26.441495265084466 31.0 25.0 34.0 10.0 34.0 3 28.10894586997795 31.0 27.0 34.0 16.0 34.0 4 32.583883997451 35.0 32.0 37.0 25.0 37.0 5 33.02745732318783 35.0 33.0 37.0 27.0 37.0 6 33.275222008341885 37.0 35.0 37.0 27.0 37.0 7 33.30988338490064 37.0 35.0 37.0 27.0 37.0 8 33.33324057256993 37.0 35.0 37.0 27.0 37.0 9 34.89462487053287 39.0 35.0 39.0 28.0 39.0 10 34.770225688584006 39.0 35.0 39.0 27.0 39.0 11 34.78352231280576 39.0 35.0 39.0 27.0 39.0 12 34.677533397991425 39.0 35.0 39.0 27.0 39.0 13 34.66774453027102 39.0 35.0 39.0 27.0 39.0 14 35.782793674264816 40.0 36.0 41.0 27.0 41.0 15 35.71207786305548 40.0 36.0 41.0 27.0 41.0 16 35.63881469857417 40.0 36.0 41.0 27.0 41.0 17 35.60353624338668 40.0 36.0 41.0 27.0 41.0 18 35.54074983080766 40.0 36.0 41.0 27.0 41.0 19 35.56909675167175 40.0 36.0 41.0 27.0 41.0 20 35.485713333014985 40.0 36.0 41.0 26.0 41.0 21 35.39367187718695 40.0 36.0 41.0 26.0 41.0 22 35.279311852355605 40.0 36.0 41.0 25.0 41.0 23 35.16492095300981 40.0 36.0 41.0 25.0 41.0 24 35.037731949935534 40.0 35.0 41.0 24.0 41.0 25 34.91814260567729 39.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.283527585048379 1101 2 3.7370149099815606 1101 3 2.313009362775034 1101 4 1.387053314631487 1101 5 0.8487634738108412 1101 6 0.6804745488335868 1101 7 0.5928749803130344 1101 8 0.36641375974812007 1101 9 0.39986574057157753 1101 10 0.6085197367809982 1101 11 0.46581168985636623 1101 12 0.5625603266977848 1101 13 0.5570903484228253 1101 14 0.7658898090913908 1101 15 0.6598678595923957 1101 16 0.7092257481642932 1101 17 0.7200366371088052 1101 18 0.8636193190940631 1101 19 0.802079599978903 1101 20 0.9239620653666094 1101 21 1.0251444195341222 1101 22 0.8416870552535016 1101 23 0.9690984949082662 1101 24 1.067161553302121 1101 25 0.9850021412085184 1102 1 1.7614798280117405 1102 2 4.2760853774434295 1102 3 2.946504432074402 1102 4 1.8934890582799824 1102 5 1.4730014053875422 1102 6 1.1750519013056717 1102 7 1.0336818630769216 1102 8 1.0418815585665513 1102 9 1.0669457324847826 1102 10 1.1615331923148347 1102 11 1.0942098298927618 1102 12 1.1505918652111617 1102 13 1.1645953465134369 1102 14 1.2047805699243312 1102 15 1.3080516994737863 1102 16 1.2563133776092243 1102 17 1.338638519535742 1102 18 1.2897666336031222 1102 19 1.1952260384181557 1102 20 1.365233939032855 1102 21 1.3394670378597056 1102 22 1.2198055268486172 1102 23 1.3342893208848565 1102 24 1.086149804981254 1102 25 1.0522214057591412 1103 1 1.7225087539751023 1103 2 4.224017768309281 1103 3 2.8246040956038847 1103 4 1.8075525632614102 1103 5 1.3801136657780972 1103 6 1.1543001150366479 1103 7 1.1577799777044149 1103 8 0.9713741400261071 1103 9 1.0996945084584269 1103 10 0.979109729512551 1103 11 1.1471179590029266 1103 12 1.2168520544922359 1103 13 1.2890474333744635 1103 14 1.3718414627744409 1103 15 1.1640077526615045 1103 16 1.0723270114470438 1103 17 1.2562299331932039 1103 18 1.286066333011128 1103 19 1.3400864403450896 1103 20 1.3640705123716117 1103 21 1.3074883374497333 1103 22 1.498513375056227 1103 23 1.2471968374349203 1103 24 1.3104154985524374 1103 25 1.1275221071404644 1104 1 1.5380024529427452 1104 2 4.062376585122244 1104 3 2.6426385982455862 1104 4 1.8281338768052962 1104 5 1.3097692358534658 1104 6 1.1950549274820972 1104 7 1.102904583268547 1104 8 1.0544340105153438 1104 9 1.1022202940621426 1104 10 1.0879093034223928 1104 11 1.164326763907198 1104 12 1.129915195113405 1104 13 0.976624824029166 1104 14 1.3183593645258043 1104 15 1.2451080822552711 1104 16 1.0932421068921698 1104 17 1.1512246430250528 1104 18 1.1408026728070269 1104 19 1.3103117410132086 1104 20 1.3154365010534832 1104 21 1.4088406406506095 1104 22 1.3354947598407634 1104 23 1.418188144298938 1104 24 1.1846522738723735 1104 25 1.0651996535177446 1105 1 1.3777499193915048 1105 2 3.8423622292201642 1105 3 2.3038105490895937 1105 4 1.4633742979412574 1105 5 0.9377275261053484 1105 6 0.8539471900370259 1105 7 0.674326201712482 1105 8 0.7051449373921557 1105 9 0.7010955137599382 1105 10 0.641898295353684 1105 11 0.5103786193240722 1105 12 0.8601533546618185 1105 13 0.6876464280729522 1105 14 0.6917962394844466 1105 15 0.7051212233962332 1105 16 0.673376619562795 1105 17 0.7942266636715658 1105 18 0.8136510120938283 1105 19 0.7618394539579114 1105 20 0.6549968259095706 1105 21 0.7687277000457939 1105 22 0.8132812237415408 1105 23 0.8200735581352703 1105 24 0.6884653969528145 1105 25 0.924552037013811 1106 1 1.1018407287000969 1106 2 3.646704991528882 1106 3 2.2418034376788327 1106 4 1.48740045806111 1106 5 0.8869106259311224 1106 6 0.8258759847230976 1106 7 0.6975771080753361 1106 8 0.6188782849727517 1106 9 0.7161235440565363 1106 10 0.6314862439580295 1106 11 0.7324526064308969 1106 12 0.7348260290235018 1106 13 0.7710856844373879 1106 14 0.9311971927693747 1106 15 1.0374131354965854 1106 16 0.7247651240786226 1106 17 0.7477463112574796 1106 18 0.8811908600922322 1106 19 0.7964866799667405 1106 20 0.9562251604954994 1106 21 0.7744030582876889 1106 22 0.6751586343939948 1106 23 0.8512314716028229 1106 24 0.8951628472442508 1106 25 0.6273946788318199 1107 1 1.584213886852833 1107 2 3.942945809317486 1107 3 2.6977156234445445 1107 4 1.7747611745136922 1107 5 1.3441018011768051 1107 6 1.2118583965695464 1107 7 0.9915744599754035 1107 8 0.818272062165498 1107 9 1.0011265503645461 1107 10 0.8829521889287122 1107 11 0.825351392847395 1107 12 0.8878468792829608 1107 13 0.9286191828104293 1107 14 0.9995088856917391 1107 15 1.1458873738720001 1107 16 1.2143190571533395 1107 17 1.1209499796359879 1107 18 1.0095497436299965 1107 19 1.1064440909825208 1107 20 1.1215427435968195 1107 21 1.1923795512803324 1107 22 1.0521045986815096 1107 23 1.130936229853333 1107 24 1.2258615077644848 1107 25 1.0992949158317558 1108 1 1.3709985177583626 1108 2 3.8701254448196956 1108 3 2.5392638725168872 1108 4 1.4141714221835073 1108 5 0.995879471067461 1108 6 0.8478254382013475 1108 7 0.6158414874900657 1108 8 0.6385091613332818 1108 9 0.6219601275916418 1108 10 0.5716898062989983 1108 11 0.5058752422140174 1108 12 0.5144679119922699 1108 13 0.5341063907261301 1108 14 0.6389644689077159 1108 15 0.5170483426065431 1108 16 0.5189745012637914 1108 17 0.6176479450455403 1108 18 0.5459553349375099 1108 19 0.485579524901091 1108 20 0.5230676247775179 1108 21 0.5043632855750033 1108 22 0.6396636066249854 1108 23 0.5649586634884045 1108 24 0.654388584562902 1108 25 0.6361837037199365 1109 1 1.2025106239893475 1109 2 3.2855841865022413 1109 3 2.1412792254786552 1109 4 1.3952949999659907 1109 5 0.9538705243974164 1109 6 0.738782189505784 1109 7 0.5448790078815833 1109 8 0.5761017792927561 1109 9 0.6205702528533266 1109 10 0.4646836338438405 1109 11 0.5643911133520803 1109 12 0.6416345323067176 1109 13 0.6159076794561784 1109 14 0.6338456619446404 1109 15 0.5210896334397361 1109 16 0.6336893244266193 1109 17 0.681590138985996 1109 18 0.6512329847278195 1109 19 0.7574781809634743 1109 20 0.7931004310792034 1109 21 0.808786324979927 1109 22 0.7401579841700396 1109 23 0.76771226201177 1109 24 0.8541016159011505 1109 25 0.7800886636840687 1110 1 0.7023258438301028 1110 2 3.3323884259668155 1110 3 1.8122379930590498 1110 4 0.9298070062416173 1110 5 0.31150340679051425 1110 6 0.2969743069977113 1110 7 0.12852775410303252 1110 8 0.02325366825515829 1110 9 -0.03571631308707879 1110 10 0.02113586135633483 1110 11 -0.044762222315782196 1110 12 0.016621146010734833 1110 13 -0.12469218428282858 1110 14 -0.05571644357024752 1110 15 -0.06799953341690923 1110 16 -0.055951620622884946 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2.095897585121694 1112 4 1.2723117044699848 1112 5 0.5927937010697093 1112 6 0.41769384800323905 1112 7 0.33619683704587544 1112 8 0.33517060920875963 1112 9 0.27448249419059323 1112 10 0.08784004851577265 1112 11 0.18981256954426584 1112 12 0.30905589776492093 1112 13 0.15718533993087647 1112 14 0.18675522977994774 1112 15 0.3004159273283591 1112 16 0.3149232057553917 1112 17 0.3091480968917253 1112 18 0.35750881477046903 1112 19 0.4616060100206525 1112 20 0.23060204570300158 1112 21 0.4109601914510108 1112 22 0.4045084139277577 1112 23 0.36865791172693463 1112 24 0.43613647665284105 1112 25 0.35608498895783214 1113 1 0.8037597734256785 1113 2 3.2674594765743237 1113 3 1.8607891121414397 1113 4 1.137061983882944 1113 5 0.6087856818507404 1113 6 0.35914339449508503 1113 7 0.13974279820710223 1113 8 0.2793685726582993 1113 9 0.01031015960261783 1113 10 0.21552633647922903 1113 11 0.1415053221989453 1113 12 0.14505471012954985 1113 13 0.29109926852921575 1113 14 0.2463640724816969 1113 15 0.24659025540459112 1113 16 0.2119003044646277 1113 17 0.3133432371759426 1113 18 0.2788628143886882 1113 19 0.22037725411635023 1113 20 0.06091447302063813 1113 21 0.06068429255725505 1113 22 0.1662605096366363 1113 23 0.3673989527185242 1113 24 0.20062597063619592 1113 25 0.2705189577118219 1114 1 0.5570760090311389 1114 2 3.049425442729344 1114 3 1.5263268067005527 1114 4 0.733061368917518 1114 5 0.08174102257786586 1114 6 -0.1487967419604459 1114 7 -0.2672802451462317 1114 8 -0.27041512419368985 1114 9 -0.3911194622684988 1114 10 -0.2841224192234506 1114 11 -0.5082830962976885 1114 12 -0.42561909735287173 1114 13 -0.438544828867947 1114 14 -0.686534159377544 1114 15 -0.5939468489489386 1114 16 -0.5473983124965542 1114 17 -0.4358470295566761 1114 18 -0.5293983939032145 1114 19 -0.47166642915802726 1114 20 -0.4550657029815426 1114 21 -0.5226317184821809 1114 22 -0.5153825996700121 1114 23 -0.6336311719501708 1114 24 -0.5350635593471154 1114 25 -0.4978573089989098 1115 1 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-2.426241016702434 2315 21 -2.433661042933416 2315 22 -2.4648643848687257 2315 23 -2.39651367492619 2315 24 -2.4098546492518125 2315 25 -2.407451127909802 2316 1 -0.5286500988361702 2316 2 1.7782690219461053 2316 3 0.34985632638600705 2316 4 -0.5340903717887997 2316 5 -0.7519030601654677 2316 6 -0.7932634169605137 2316 7 -1.4542535010458018 2316 8 -1.3893773124892377 2316 9 -1.614220333679647 2316 10 -1.710308711823167 2316 11 -1.4877751029601711 2316 12 -1.8366665691157351 2316 13 -1.6749177007624638 2316 14 -1.842975735349171 2316 15 -1.8482535740276873 2316 16 -2.0507507123569297 2316 17 -1.8899781416718113 2316 18 -2.3361423084211097 2316 19 -2.1251473647918075 2316 20 -1.9285645105795481 2316 21 -2.3680556174520078 2316 22 -2.0601486166168215 2316 23 -2.2838349284873445 2316 24 -2.1647291119310736 2316 25 -2.0411403149527274 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93257.0 3 2.0 4 860.0 5 4062.0 6 4921.0 7 4299.0 8 4174.0 9 3962.0 10 3905.0 11 3747.0 12 3597.0 13 3666.0 14 3663.0 15 3822.0 16 4037.0 17 4473.0 18 5017.0 19 6305.0 20 8081.0 21 10351.0 22 12920.0 23 15236.0 24 17888.0 25 20240.0 26 22820.0 27 25815.0 28 29865.0 29 36065.0 30 44850.0 31 56636.0 32 71922.0 33 94816.0 34 128607.0 35 180661.0 36 283105.0 37 359336.0 38 852205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.931181942278656 18.121281679310123 12.687861128903979 25.25967524950724 2 16.979927651390582 20.961683818326133 38.632986627043095 23.425401903240193 3 18.927600498602825 24.229824945619686 29.338033943852842 27.504540611924643 4 12.644019318389518 16.73419266026343 37.80448446147437 32.81730355987268 5 13.75274371518384 37.23194746557286 35.38873895309873 13.626569866144573 6 32.193432537950954 36.52837903035911 18.07620488821779 13.20198354347214 7 28.357459365022386 31.326723168400306 22.044526813074985 18.271290653502323 8 25.514040082529636 36.04229067490865 20.57736988656292 17.866299355998795 9 26.485187642949004 15.539019623018063 19.90726942500951 38.068523309023426 10 15.115380118788666 28.828192795287972 33.985842182655276 22.070584903268088 11 34.75498808655402 22.575815457675567 23.841917546110057 18.827278909660347 12 23.179062303946836 24.524408979461448 31.312850219908878 20.983678496682842 13 28.97779010928755 21.473759956001757 25.682779595486227 23.865670339224465 14 21.71647480557289 21.279003518871324 27.42430804038222 29.580213635173564 15 23.6631746904727 29.245122238377597 23.579154845158136 23.512548225991566 16 23.09866506832736 27.699955705363273 26.49383250699411 22.70754671931526 17 22.39583762146034 27.580080257271156 27.232103896446052 22.79197822482245 18 22.34236296243848 27.214773002336585 28.379400853289248 22.063463181935692 19 23.64637895461364 26.836457285315092 27.190690881068075 22.32647287900319 20 23.645061641997245 26.812622160162164 27.59543518245603 21.946881015384566 21 23.74638870204044 26.41732896094241 27.01287923259764 22.823403104419512 22 23.425887846966216 26.87691192398369 27.176233130506265 22.52096709854382 23 23.336606049378904 26.637891465209385 27.0502948531909 22.975207632220815 24 23.262607309609063 26.51155572278121 27.497935522434265 22.727901445175462 25 23.512177731818205 26.529770441810186 27.29846351949705 22.65958830687456 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 259.0 1 259.0 2 431.0 3 603.0 4 603.0 5 603.0 6 1552.5 7 2502.0 8 2502.0 9 2502.0 10 3550.0 11 4598.0 12 4598.0 13 4598.0 14 7462.0 15 10326.0 16 10326.0 17 10326.0 18 18318.5 19 26311.0 20 26311.0 21 26311.0 22 40971.5 23 55632.0 24 55632.0 25 55632.0 26 79117.0 27 102602.0 28 102602.0 29 102602.0 30 132311.5 31 162021.0 32 162021.0 33 162021.0 34 185138.0 35 208255.0 36 208255.0 37 208255.0 38 230178.5 39 252102.0 40 252102.0 41 252102.0 42 271596.0 43 291090.0 44 291090.0 45 291090.0 46 303734.5 47 316379.0 48 316379.0 49 316379.0 50 312087.5 51 307796.0 52 307796.0 53 307796.0 54 281617.0 55 255438.0 56 255438.0 57 255438.0 58 222886.0 59 190334.0 60 190334.0 61 190334.0 62 156916.0 63 123498.0 64 123498.0 65 123498.0 66 95948.0 67 68398.0 68 68398.0 69 68398.0 70 50284.5 71 32171.0 72 32171.0 73 32171.0 74 22321.0 75 12471.0 76 12471.0 77 12471.0 78 8486.5 79 4502.0 80 4502.0 81 4502.0 82 2927.0 83 1352.0 84 1352.0 85 1352.0 86 871.0 87 390.0 88 390.0 89 390.0 90 250.5 91 111.0 92 111.0 93 111.0 94 73.5 95 36.0 96 36.0 97 36.0 98 23.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.3632687136606965 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 2.469961155744224E-4 22 0.0010291504815600934 23 5.351582504112485E-4 24 8.233203852480746E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2429188.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.0454511917073 #Duplication Level Percentage of deduplicated Percentage of total 1 80.81284759280763 58.22198066910504 2 12.99683804358899 18.727261218318304 3 3.4679331253930266 7.495464201648247 4 1.260553971934418 3.6326871863805557 5 0.5590087124533653 2.013701745439903 6 0.29076629952291744 1.2569033544283013 7 0.15839955301951325 0.7988377086098916 8 0.10028624411185533 0.5780134164288253 9 0.07221427741403944 0.4682439178899829 >10 0.2552711727773375 3.0725960538084673 >50 0.013233353619453688 0.6435841524407373 >100 0.010976257177645433 1.5565372229257304 >500 9.221496164465821E-4 0.4567106877044292 >1k 6.9161221232568E-4 0.8462372775090959 >5k 5.7634351027140004E-5 0.2312411873625199 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5569 0.2292535612723264 No Hit TATCAACGCAGAGTACTTTTTTTTT 3413 0.14049962374258393 No Hit GGTATCAACGCAGAGTACTTTTTTT 3315 0.13646535385486838 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 8.233203852480747E-5 0.0 8 0.0 0.0 0.0 8.233203852480747E-5 0.0 9 0.0 0.0 0.0 1.6466407704961495E-4 0.0 10 0.0 0.0 0.0 1.6466407704961495E-4 0.0 11 0.0 0.0 0.0 2.469961155744224E-4 0.0 12 0.0 0.0 0.0 2.881621348368261E-4 4.1166019262403737E-5 13 0.0 0.0 0.0 2.881621348368261E-4 8.233203852480747E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGTCGT 55 0.0031770056 12.035345 7 GTCCTAA 560 0.0 11.12403 1 AAACGGG 60 0.006082218 11.032399 19 ATAGGAC 155 3.4015102E-10 10.981559 3 GCGCCAC 165 8.731149E-11 10.889122 13 TTAGACT 260 0.0 10.547459 4 TAGGACT 225 0.0 10.507048 4 TCCAACG 175 2.564775E-10 10.266886 18 GTACTAG 175 1.2878445E-9 10.170542 1 GCGATAA 75 0.0027516193 10.086765 11 ATACCAT 285 0.0 9.954044 6 GGTATCA 1415 0.0 9.922967 1 AGCGTAC 90 7.7191764E-4 9.888027 1 CCTAGTA 140 6.130522E-7 9.888027 2 CGCCCTA 155 4.3661203E-8 9.761386 16 GTATCAA 2715 0.0 9.760556 1 TAGGACC 370 0.0 9.71192 4 GCGTACA 115 7.177204E-5 9.458113 2 GCCGGTT 110 7.1922695E-5 9.456343 11 CTAGTAC 150 2.8328031E-7 9.456343 3 >>END_MODULE