FastQCFastQC Report
Thu 26 May 2016
SRR1511753_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511753_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429188
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72650.2990711299413631No Hit
TATCAACGCAGAGTACTTTTTTTTT45110.18569991289270324No Hit
GGTATCAACGCAGAGTACTTTTTTT43350.1784546935025202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2400.014.24550718
ATACGAT500.001499811913.3001883
CCAACGA1552.5465852E-1111.6414919
GCCGGTT1701.2732926E-1111.17294711
TTAGGAC5800.011.1380883
AGGACGG1053.467645E-610.8572965
GGCGAGG4550.010.8537219
TATCGCG700.001495855410.853727
ACCGTCC803.783328E-410.6841318
AGGACCG1355.6903446E-810.5557055
ATCGCGC909.5496915E-510.5522288
GTGTAAG2900.010.5286481
AGAACCG1002.3995248E-510.4501485
GGTATCA14900.010.4381121
TAGGACG10000.010.3551454
ACTGTTC7400.010.1386958
CCGTGCG856.614939E-410.0556529
TCGCGCG856.614939E-410.0556529
GTCCTAA6400.09.9888491
CGGTTTC2300.09.90991913
Produced by FastQC (version 0.11.5)