FastQCFastQC Report
Thu 26 May 2016
SRR1511752_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511752_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562208
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT140272.494984062837953No Hit
TATCAACGCAGAGTACTTTTTTTTT90421.6083015538732994No Hit
GGTATCAACGCAGAGTACTTTTTTT86951.546580624964426No Hit
GTACTGGTTCACTATCGGTCAGTCA57741.0270220274346862No Hit
ACGCAGAGTACTTTTTTTTTTTTTT43240.769110364847174No Hit
GTACTTTTTTTTTTTTTTTTTTTTT41730.7422519779156469No Hit
ATCATTAACTGAATCCATAGGTTAA38810.6903139051738858No Hit
GGATACCACGTGTCCCGCCCTACTC34820.6193437304342877No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30760.5471284649097843No Hit
GTACATGGGGGTTAAGCGACTAAGC29460.5240053503329728No Hit
GTACATAAGCAGTGGTATCAACGCA24470.43524816438044284No Hit
ATACAGGGTGACAGCCCCGTACACA23390.41603819227047645No Hit
GTATTTAGCCTTGGAGGATGGTCCC22020.3916699869087597No Hit
GGTTAATGAGGCGAACCGGGGGAAC21910.38971341567533724No Hit
GTATCAACGCAGAGTACATGGGGGT21480.38206500085377654No Hit
TATTCAGACAGGATACCACGTGTCC21430.38117565029312994No Hit
GGTATCAACGCAGAGTACATGGGGG20910.3719264044624054No Hit
GTTCACTATCGGTCAGTCAGGAGTA20810.3701477033411122No Hit
AGCGTACACGGTGGATGCCCTGGCA20290.36089845751038757No Hit
CCCATATTCAGACAGGATACCACGT20140.35823040582844784No Hit
CTATCGGTCAGTCAGGAGTATTTAG20050.356629574819284No Hit
GTGGTATCAACGCAGAGTACATGGG20050.356629574819284No Hit
GTGGATGCCCTGGCAGTCAGAGGCG19790.35200495190392167No Hit
ACCCTGTATCGCGCGCCTTTCCAGA17420.3098497353292731No Hit
GTCAGGAGTATTTAGCCTTGGAGGA17000.30237919061984175No Hit
GCAGAGTACTTTTTTTTTTTTTTTT16910.3007783596106779No Hit
GCCTTGGAGGATGGTCCCCCCATAT16650.2961537366953156No Hit
TCTAAGTACCCCGAGGAAAAGAAAT15110.2687617394274005No Hit
GTTATAACCGGCGATTTCCGAATGG14010.2491960270931755No Hit
TATCAACGCAGAGTACATGGGGGTT13550.24101400193522685No Hit
GGTTCACTATCGGTCAGTCAGGAGT13390.2381680801411577No Hit
GCCCAGAGCCTGAATCAGTGTGTGT13260.2358557686834766No Hit
TCACGGTACTGGTTCACTATCGGTC13190.23461067789857132No Hit
CGGTACTGGTTCACTATCGGTCAGT12750.22678439296488132No Hit
GTTAATGATAGTGTGTCGAAACACA12720.22625078262849338No Hit
ACGCAGAGTACATGGGGGTTAAGCG12420.22091467926461378No Hit
GTGCTAATCTGCGATAAGCGTCGGT12160.21629005634925152No Hit
GGTGATATGAACCGTTATAACCGGC12100.21522283567647563No Hit
ATCAACGCAGAGTACTTTTTTTTTT12060.21451135522795833No Hit
GAGTACATGGGGGTTAAGCGACTAA11970.21291052421879447No Hit
ACTTAGATGTTTCAGTTCCCCCGGT11880.2113096932096306No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG11860.21095395298537195No Hit
GATATGAACCGTTATAACCGGCGAT11600.20632933007000967No Hit
CCCATGTACTCTGCGTTGATACCAC11560.20561784962149238No Hit
CACTATCATTAACTGAATCCATAGG11140.198147304912061No Hit
GTGGTATCAACGCAGAGTACTTTTT10940.19458990266947465No Hit
GCTTATGTACTCTGCGTTGATACCA10570.18800870852068985No Hit
CATCTAAGTACCCCGAGGAAAAGAA10360.18427343616597416No Hit
CTATCATTAACTGAATCCATAGGTT10340.18391769594171553No Hit
GGAGTATTTAGCCTTGGAGGATGGT10300.18320621549319824No Hit
ATCCATAGGTTAATGAGGCGAACCG9640.17146678809266322No Hit
GCGTACACGGTGGATGCCCTGGCAG9480.1686208662985941No Hit
GTCCCGCCCTACTCATCGAGCTCAC9390.16702003528943024No Hit
CCGTTATAACCGGCGATTTCCGAAT9200.1636405031589732No Hit
CACACACACTGATTCAGGCTCTGGG9180.16328476293471456No Hit
GCCTGAATCAGTGTGTGTGTTAGTG9140.16257328248619726No Hit
GAATCAGTGTGTGTGTTAGTGGAAG9020.16043884114064544No Hit
GGTATCAACGCAGAGTACATGGGGA8930.15883801013148158No Hit
TCGGTAAGGTGATATGAACCGTTAT8600.15296829643121407No Hit
GTACTTAGATGTTTCAGTTCCCCCG8110.14425266093687747No Hit
GCCCTACTCATCGAGCTCACAGCAT8110.14425266093687747No Hit
GTCCCCCCATATTCAGACAGGATAC7950.14140673914280835No Hit
TCATTAACCTATGGATTCAGTTAAT7780.13838294723660993No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG7510.13358045420911832No Hit
GTACATGGGAAGCAGTGGTATCAAC7460.13269110364847175No Hit
TTTGTGTACGGGGCTGTCACCCTGT7430.1321574933120838No Hit
GTGTTTCGACACACTATCATTAACT7300.12984518185440264No Hit
CATTAACTGAATCCATAGGTTAATG7110.12646564972394558No Hit
AGCTCACAGCATGTGCATTTTTGTG7040.12522055893904036No Hit
CATGTACTCTGCGTTGATACCACTG6760.12024019579941943No Hit
CCCCAGTAGCGGCGAGCGAACGGGG6750.12006232568729011No Hit
AGTCAGGAGTATTTAGCCTTGGAGG6670.11863936479025557No Hit
GTACATGGGCATCTAAGTACCCCGA6630.1179278843417383No Hit
GATTCAGGCTCTGGGCTGCTCCCCG6570.11686066366896238No Hit
GGGTACTTAGATGTTTCAGTTCCCC6480.11525983265979851No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA6350.11294752120211736No Hit
ATTTAGCCTTGGAGGATGGTCCCCC6190.11010159940804827No Hit
TTCCAGACGCTTCCACTAACACACA6130.10903437873527236No Hit
TAAGTACCCCGAGGAAAAGAAATCA5990.10654419716546189No Hit
CTTATGTACTCTGCGTTGATACCAC5910.10512123626842734No Hit
CCCTGGCAGTCAGAGGCGATGAAGG5890.1047654960441687No Hit
GGGTGACAGCCCCGTACACAAAAAT5860.10423188570778076No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA5820.10352040525926348No Hit
GTATCGCGCGCCTTTCCAGACGCTT5650.10049661335306506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCT250.006187231318.9140874
TGGGTCG250.006187231318.9140876
GTCAACG1000.017.786732
TTCAACG452.5189906E-517.567142
GATATCA452.5189906E-517.567141
GCACCAC402.8541553E-416.5498266
CTGCACC350.00223450516.2120744
AATCGCC551.1751499E-515.47516218
TTTCAAC455.007961E-415.3712471
TGTCAAC1201.8189894E-1214.8222751
GTATTGA400.00405984214.8222741
GTCCAGA500.001112522913.8341231
GAAATCG552.0333214E-413.75569916
AAATCGC552.0333214E-413.75569917
CCGGGGT500.001549486513.23986158
GAATCAA1750.012.9871371
GTTATCA707.53226E-512.7048061
ATCAGTA905.688544E-712.6093927
TCAGTAT854.134923E-612.2385278
TTATCGC701.1355434E-412.15905613
Produced by FastQC (version 0.11.5)