FastQCFastQC Report
Thu 26 May 2016
SRR1511752_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511752_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562208
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT185553.3003799305595085No Hit
TATCAACGCAGAGTACTTTTTTTTT121952.1691260174170415No Hit
GGTATCAACGCAGAGTACTTTTTTT113532.019359383004155No Hit
GTACTGGTTCACTATCGGTCAGTCA63911.1367678866184756No Hit
ACGCAGAGTACTTTTTTTTTTTTTT59621.060461608514998No Hit
GTACTTTTTTTTTTTTTTTTTTTTT50080.890773521543628No Hit
ATCATTAACTGAATCCATAGGTTAA43580.7751579486595708No Hit
GGATACCACGTGTCCCGCCCTACTC39210.6974287096590586No Hit
GAGTACTTTTTTTTTTTTTTTTTTT38490.6846220615857477No Hit
GTACATGGGGGTTAAGCGACTAAGC31770.5650933462348455No Hit
GTACATAAGCAGTGGTATCAACGCA27640.49163298992543686No Hit
TATTCAGACAGGATACCACGTGTCC24830.4416514884170983No Hit
GGTTAATGAGGCGAACCGGGGGAAC24650.43844982639877056No Hit
GTATCAACGCAGAGTACATGGGGGT24350.433113723034891No Hit
GTATTTAGCCTTGGAGGATGGTCCC24310.4324022425863737No Hit
ATACAGGGTGACAGCCCCGTACACA24310.4324022425863737No Hit
GGTATCAACGCAGAGTACATGGGGG23680.42119642552222664No Hit
AGCGTACACGGTGGATGCCCTGGCA23340.4151488417098298No Hit
GTTCACTATCGGTCAGTCAGGAGTA22840.4062553361033639No Hit
CCCATATTCAGACAGGATACCACGT22350.39753970060902727No Hit
GCAGAGTACTTTTTTTTTTTTTTTT22100.3930929478057943No Hit
GTGGATGCCCTGGCAGTCAGAGGCG22100.3930929478057943No Hit
GTGGTATCAACGCAGAGTACATGGG21970.3907806363481131No Hit
CTATCGGTCAGTCAGGAGTATTTAG21720.3863338835448802No Hit
ACCCTGTATCGCGCGCCTTTCCAGA20100.3575189253799306No Hit
GTCAGGAGTATTTAGCCTTGGAGGA19610.34880328988559395No Hit
GCCTTGGAGGATGGTCCCCCCATAT19070.3391983038306107No Hit
ATCAACGCAGAGTACTTTTTTTTTT17380.30913825488075586No Hit
TCTAAGTACCCCGAGGAAAAGAAAT16860.2998890090500313No Hit
TCACGGTACTGGTTCACTATCGGTC15700.2792560760430303No Hit
CGGTACTGGTTCACTATCGGTCAGT15500.27569867380044394No Hit
GTGGTATCAACGCAGAGTACTTTTT15390.2737421025670215No Hit
GTTATAACCGGCGATTTCCGAATGG15120.26893960953952983No Hit
TATCAACGCAGAGTACATGGGGGTT14850.26413711651203825No Hit
ACGCAGAGTACATGGGGGTTAAGCG14550.2588010131481587No Hit
GGTTCACTATCGGTCAGTCAGGAGT14400.2561329614662189No Hit
ACTTAGATGTTTCAGTTCCCCCGGT14120.25115259832659803No Hit
GCCCAGAGCCTGAATCAGTGTGTGT13840.24617223518697706No Hit
GATATGAACCGTTATAACCGGCGAT13810.24563862485058907No Hit
GAGTACATGGGGGTTAAGCGACTAA13780.24510501451420114No Hit
GTTAATGATAGTGTGTCGAAACACA13730.24421566395355457No Hit
GGTGATATGAACCGTTATAACCGGC13600.2419033524958734No Hit
GTGCTAATCTGCGATAAGCGTCGGT12920.22980818487107973No Hit
CACTATCATTAACTGAATCCATAGG12460.22162615971313107No Hit
CTATCATTAACTGAATCCATAGGTT12370.2200253287039672No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG12160.21629005634925152No Hit
GGAGTATTTAGCCTTGGAGGATGGT12000.2134441345551824No Hit
CCCATGTACTCTGCGTTGATACCAC11930.21219904377027718No Hit
CATCTAAGTACCCCGAGGAAAAGAA11870.21113182309750125No Hit
GCTTATGTACTCTGCGTTGATACCA11730.20864164152769082No Hit
ATCCATAGGTTAATGAGGCGAACCG11450.20366127838806988No Hit
CCGTTATAACCGGCGATTTCCGAAT11420.20312766805168195No Hit
GGTATCAACGCAGAGTACATGGGGA10370.1844513062781035No Hit
GTACTTAGATGTTTCAGTTCCCCCG10230.181961124708293No Hit
TCGGTAAGGTGATATGAACCGTTAT10090.17947094313848255No Hit
GCCTGAATCAGTGTGTGTGTTAGTG9890.17591354089589617No Hit
CACACACACTGATTCAGGCTCTGGG9850.1752020604473789No Hit
GCGTACACGGTGGATGCCCTGGCAG9490.16879873641072343No Hit
GAATCAGTGTGTGTGTTAGTGGAAG9440.16790938585007684No Hit
TCATTAACCTATGGATTCAGTTAAT9110.16203967214980933No Hit
GTCCCGCCCTACTCATCGAGCTCAC8930.15883801013148158No Hit
GTACATGGGAAGCAGTGGTATCAAC8640.15367977687973133No Hit
GCCCTACTCATCGAGCTCACAGCAT8630.15350190676760203No Hit
TTTGTGTACGGGGCTGTCACCCTGT8500.15118959530992088No Hit
CATTAACTGAATCCATAGGTTAATG7970.14176247936706698No Hit
AGCTCACAGCATGTGCATTTTTGTG7950.14140673914280835No Hit
GTGTTTCGACACACTATCATTAACT7840.13945016790938583No Hit
CCCCAGTAGCGGCGAGCGAACGGGG7820.1390944276851272No Hit
GTCCCCCCATATTCAGACAGGATAC7770.13820507712448063No Hit
GTACATGGGCATCTAAGTACCCCGA7360.13091240252717856No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG7230.1286000910694974No Hit
TAAGTACCCCGAGGAAAAGAAATCA7160.12735500028459218No Hit
AGTCAGGAGTATTTAGCCTTGGAGG7080.12593203938755762No Hit
GATTCAGGCTCTGGGCTGCTCCCCG7010.12468694860265239No Hit
GTACATGGGATAAGCAGTGGTATCA6980.12415333826626444No Hit
CTTATGTACTCTGCGTTGATACCAC6890.12255250725710057No Hit
GGGTACTTAGATGTTTCAGTTCCCC6850.12184102680858329No Hit
TTCCAGACGCTTCCACTAACACACA6780.12059593602367806No Hit
GGGAAACCCAGTGTGTTTCGACACA6760.12024019579941943No Hit
CATGTACTCTGCGTTGATACCACTG6640.1181057544538676No Hit
CCCTGGCAGTCAGAGGCGATGAAGG6550.11650492344470373No Hit
GGTATCAACGCAGAGTACATGGGCA6510.11579344299618648No Hit
CCTATGGATTCAGTTAATGATAGTG6460.11490409243553987No Hit
GAGTACATAAGCAGTGGTATCAACG6420.11419261198702259No Hit
GTACATGATAAGCAGTGGTATCAAC6360.11312539131424669No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA6350.11294752120211736No Hit
ATTCAGACAGGATACCACGTGTCCC6260.1113466901929535No Hit
ATTTAGCCTTGGAGGATGGTCCCCC6220.11063520974443622No Hit
CAACGCAGAGTACTTTTTTTTTTTT6160.10956798907166031No Hit
GGTATCAACGCAGAGTACATGGGAT6130.10903437873527236No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA6070.10796715806249645No Hit
GGGGTTAAGCGACTAAGCGTACACG6050.10761141783823781No Hit
GTATCGCGCGCCTTTCCAGACGCTT6010.10689993738972052No Hit
CAGTAGCGGCGAGCGAACGGGGAGC6000.1067220672775912No Hit
CCACGTGTCCCGCCCTACTCATCGA5930.10547697649268598No Hit
GGGTGACAGCCCCGTACACAAAAAT5760.10245318458648757No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT5650.10049661335306506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAG350.002165915716.2879283
TAGGCAG350.002165915716.2879285
TAGTAGA350.002165915716.2879284
TTATGTT456.745881E-414.7797854
TATTATG456.745881E-414.7797852
ACCTTAG400.005142555614.3041161
ATAAGTG400.005268807514.2519355
ATTATGT400.005268807514.2519353
TCATGTG400.005284380614.24559813
GTAACCG400.005284380614.2455989
CGAATGG2200.012.51885819
AGGGTGA4400.012.0925515
AGGACCG550.003071358712.0871736
AGAACTT1052.7186434E-711.7582713
TATAAAA658.0003275E-411.6938952
CACTTTG658.0302433E-411.68869614
ACAGGGT4400.011.6606743
TCGCTCG907.462404E-611.60752415
GAAATCG855.3253636E-511.173017516
AAATCGC855.3253636E-511.173017517
Produced by FastQC (version 0.11.5)