FastQCFastQC Report
Thu 26 May 2016
SRR1511751_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511751_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114236
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62830.2971759065686139No Hit
GGTATCAACGCAGAGTACTTTTTTT37230.17609197837895108No Hit
TATCAACGCAGAGTACTTTTTTTTT36060.17055806447340788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG655.6943238E-513.0935945
GGTATCA14300.011.3386011
ACCCGTG855.5795837E-511.1252767
TCGAATT600.00608109511.03256514
GTCCTAA3050.011.0212481
TCCAACG1951.8189894E-1210.66885518
GGCGAGG3900.010.66885519
CGGTTTC1605.8935257E-1010.63854513
GGACCGA803.9353027E-410.6385456
CGACTAA803.9353027E-410.63854517
GTCAACG854.567053E-410.4682792
ACGGTAC1002.524161E-510.4021333
GTCCTAC6200.010.3650961
TGGCGAG7150.010.18390718
TGCCGGT1951.6370905E-1110.18390710
ACCGACT750.00275102410.0869178
GCGACTA856.8792753E-410.01274816
TAGGACC3400.010.0127484
CTTATAC2002.7284841E-119.9293093
GCACCGT1054.318492E-59.9067946
Produced by FastQC (version 0.11.5)