##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511751_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2114236 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.255641754279086 31.0 30.0 34.0 23.0 34.0 2 26.574989263261056 31.0 25.0 34.0 10.0 34.0 3 28.25452125495924 31.0 27.0 34.0 16.0 34.0 4 32.742020758325936 35.0 32.0 37.0 26.0 37.0 5 33.19015190357179 35.0 35.0 37.0 27.0 37.0 6 33.44567210093859 37.0 35.0 37.0 28.0 37.0 7 33.486465560136146 37.0 35.0 37.0 28.0 37.0 8 33.508002417894694 37.0 35.0 37.0 28.0 37.0 9 35.08719840169215 39.0 35.0 39.0 28.0 39.0 10 34.966162244896026 39.0 35.0 39.0 28.0 39.0 11 34.98128543833328 39.0 35.0 39.0 28.0 39.0 12 34.87504848086969 39.0 35.0 39.0 27.0 39.0 13 34.871284473445726 39.0 35.0 39.0 27.0 39.0 14 36.00152206281607 40.0 36.0 41.0 27.0 41.0 15 35.92640462086541 40.0 36.0 41.0 27.0 41.0 16 35.85962068567558 40.0 36.0 41.0 27.0 41.0 17 35.82841603302564 40.0 36.0 41.0 27.0 41.0 18 35.76142161991377 40.0 36.0 41.0 27.0 41.0 19 35.788351915301796 40.0 36.0 41.0 27.0 41.0 20 35.71093577065191 40.0 36.0 41.0 27.0 41.0 21 35.623469659962275 40.0 36.0 41.0 27.0 41.0 22 35.509356098373125 40.0 36.0 41.0 27.0 41.0 23 35.396506350284454 40.0 36.0 41.0 26.0 41.0 24 35.26724547306923 40.0 36.0 41.0 25.0 41.0 25 35.14813956436273 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9642086364857505 1101 2 3.502848587197615 1101 3 2.121690647729416 1101 4 1.1231592728796187 1101 5 0.7822186163313631 1101 6 0.4202145196882654 1101 7 0.415591206899407 1101 8 0.1256302043998403 1101 9 0.3387728348492871 1101 10 0.43516460459463957 1101 11 0.394678797978564 1101 12 0.42742303926477376 1101 13 0.3235257204123769 1101 14 0.405774470331437 1101 15 0.3919296430905561 1101 16 0.32637333106879396 1101 17 0.5681027530943865 1101 18 0.5152229493132694 1101 19 0.5032907742074215 1101 20 0.5720565415162824 1101 21 0.6221961362818504 1101 22 0.4641678974754697 1101 23 0.5033743594496016 1101 24 0.5758442152073613 1101 25 0.6487682405859303 1102 1 1.5871513540461564 1102 2 4.153425588340355 1102 3 2.7830333310311097 1102 4 1.7435196815437877 1102 5 1.2578403767128066 1102 6 0.9968528368816791 1102 7 0.9884185359636959 1102 8 0.9026200840015903 1102 9 1.0195435502227639 1102 10 1.1311766326867811 1102 11 1.1580631595815873 1102 12 1.238243981191566 1102 13 1.2700340304959354 1102 14 1.281576088938138 1102 15 1.3221958637682718 1102 16 1.3064962400556084 1102 17 1.3070258681027482 1102 18 1.2176106757785874 1102 19 1.189328472866876 1102 20 1.2444720034574317 1102 21 1.1735287648869388 1102 22 0.8587370120755509 1102 23 1.0979266380057666 1102 24 0.9219102711616927 1102 25 0.9116378416127517 1103 1 1.6258900907314704 1103 2 4.229108965901652 1103 3 2.788052069745003 1103 4 1.7371114231757545 1103 5 1.3880096206055583 1103 6 1.2193093207274046 1103 7 1.010532326711207 1103 8 0.8710469038677644 1103 9 1.0985339104835816 1103 10 0.926462237810199 1103 11 1.150932647302703 1103 12 1.116998490648676 1103 13 1.0902717275344926 1103 14 1.2789563158768686 1103 15 1.1264009485497724 1103 16 1.1435831093940365 1103 17 1.1159935550041098 1103 18 1.164704650841884 1103 19 1.1417460433243818 1103 20 1.1385120838484255 1103 21 1.2630090440786716 1103 22 1.226606713481253 1103 23 1.2149320564771173 1103 24 1.3939332602045127 1103 25 1.1525313167110909 1104 1 1.7018038156511714 1104 2 4.201030133080675 1104 3 2.6905794190088983 1104 4 1.8564293035756734 1104 5 1.368659090082069 1104 6 1.261961210797672 1104 7 1.1784157619417286 1104 8 1.1598935779030413 1104 9 1.1666939146837976 1104 10 1.1797145346670916 1104 11 1.1801785716430118 1104 12 1.0981267739139327 1104 13 0.8802357248940496 1104 14 1.1727490734559893 1104 15 1.1706126066674258 1104 16 1.0998778836788645 1104 17 1.1127941710359224 1104 18 1.1785823206184247 1104 19 1.2545423760100007 1104 20 1.2580139971894226 1104 21 1.3202490312373882 1104 22 1.2733543619340324 1104 23 1.2702718283200198 1104 24 1.3111698456250593 1104 25 1.040783944444442 1105 1 1.5056765104865413 1105 2 3.9634550594450566 1105 3 2.4164775309475566 1105 4 1.675740761306571 1105 5 1.2377879033062484 1105 6 1.0431884911018798 1105 7 0.9295812407604203 1105 8 0.8210732138557404 1105 9 0.8873674259217239 1105 10 0.6751200529494099 1105 11 0.7428282591399977 1105 12 1.054478610650115 1105 13 0.9265908824394415 1105 14 0.805032973687716 1105 15 1.0237592755670946 1105 16 0.988003916866603 1105 17 1.1218101810927834 1105 18 0.9561525369510164 1105 19 1.01547124260712 1105 20 1.040354909428494 1105 21 0.988483495497988 1105 22 1.1255060923938913 1105 23 1.0806677089514096 1105 24 0.9810767142741383 1105 25 1.017509584821937 1106 1 1.3752563425178224 1106 2 3.934428027253883 1106 3 2.3884140711482793 1106 4 1.4019578429130632 1106 5 0.9431847292612758 1106 6 0.7932689353298485 1106 7 0.6901475681253473 1106 8 0.7616895485178006 1106 9 0.6291209401182982 1106 10 0.6187161942053692 1106 11 0.7495000015590421 1106 12 0.6569071178572727 1106 13 0.9731459646097775 1106 14 1.1058407568206334 1106 15 1.0922154086995093 1106 16 0.8459936258665692 1106 17 0.7467416789496539 1106 18 0.7813511858396538 1106 19 0.8485396521441402 1106 20 0.7502299439359916 1106 21 0.7271191731138629 1106 22 0.8632954205365948 1106 23 0.7889869022701745 1106 24 0.7318344139150312 1106 25 0.7646184280603094 1107 1 1.2030102738161688 1107 2 3.6265114190798293 1107 3 2.205897492954165 1107 4 1.326425639458705 1107 5 0.9339272324912571 1107 6 0.7346373293329123 1107 7 0.545533012625576 1107 8 0.5193225369231769 1107 9 0.5932169853832576 1107 10 0.6616089254970561 1107 11 0.6968518923178451 1107 12 0.6742151807961179 1107 13 0.7089507000129984 1107 14 0.7828110457463708 1107 15 0.8159038457214081 1107 16 0.8859395192093587 1107 17 0.8019547387834365 1107 18 0.7316368280647367 1107 19 0.9847407890025792 1107 20 1.0158248673324763 1107 21 1.0801633269062094 1107 22 0.9062482306802835 1107 23 1.0556572966015807 1107 24 0.893665426975339 1107 25 1.0670440670970507 1108 1 1.371552634689504 1108 2 3.9287748822900426 1108 3 2.4905273255946483 1108 4 1.4495472728544385 1108 5 1.1493314420848506 1108 6 0.7626701223650088 1108 7 0.5983263506097032 1108 8 0.6674380772523705 1108 9 0.6797007876935908 1108 10 0.6204587716162919 1108 11 0.503693295963032 1108 12 0.5727527954913114 1108 13 0.6726870868196073 1108 14 0.7296876461013966 1108 15 0.6707931492567667 1108 16 0.7126295187850644 1108 17 0.7215741170044936 1108 18 0.7877833261055827 1108 19 0.7039291136016956 1108 20 0.5663386090476337 1108 21 0.5605957027482589 1108 22 0.8744313226426641 1108 23 0.6366654795652735 1108 24 0.7593303310107729 1108 25 0.8048914828967924 1109 1 1.1940566258895053 1109 2 3.1806099233179843 1109 3 1.9820261265265735 1109 4 1.282734698695549 1109 5 0.894585387166849 1109 6 0.6542306417106545 1109 7 0.6408535260699182 1109 8 0.5481327396344255 1109 9 0.5195241955976471 1109 10 0.5242051476522391 1109 11 0.46790982511074475 1109 12 0.511958108285242 1109 13 0.5429839045635418 1109 14 0.5322816414235518 1109 15 0.5120909667934512 1109 16 0.5556661558375211 1109 17 0.6228467461405955 1109 18 0.48931854317903145 1109 19 0.5489043009774335 1109 20 0.6831779430353535 1109 21 0.6737788470167629 1109 22 0.7087419780648858 1109 23 0.6923983458731655 1109 24 0.55303072568978 1109 25 0.6260634039792095 1110 1 1.146543041527579 1110 2 3.653217686802524 1110 3 2.2173246352848004 1110 4 1.4193846995635582 1110 5 0.8418923850662665 1110 6 0.6581458023793729 1110 7 0.5471774154506761 1110 8 0.5486331475992898 1110 9 0.5072210169437525 1110 10 0.5502333059078808 1110 11 0.4605768737019673 1110 12 0.4574548706066466 1110 13 0.3662201075390783 1110 14 0.46040561066126884 1110 15 0.5649805732375626 1110 16 0.5103772819941454 1110 17 0.5349437723371508 1110 18 0.553266601439887 1110 19 0.547658706129937 1110 20 0.6305626278607903 1110 21 0.30207121026172956 1110 22 0.3319078722023434 1110 23 0.48939416206329156 1110 24 0.49273482096259613 1110 25 0.30889068728043156 1111 1 0.35312554836782084 1111 2 2.852145477225161 1111 3 1.376548572859253 1111 4 0.5402681116840853 1111 5 0.11143512754715346 1111 6 -0.08648456725099862 1111 7 -0.3401118416839424 1111 8 -0.2300010189214774 1111 9 -0.525378685759037 1111 10 -0.918306745106392 1111 11 -0.901643380270869 1111 12 -0.7743639978101555 1111 13 -0.8526774038842078 1111 14 -0.891129881338145 1111 15 -1.0396690726491968 1111 16 -0.8416834579531951 1111 17 -0.9107123756385889 1111 18 -0.9615673618476848 1111 19 -0.9273190652395868 1111 20 -0.9847207142266754 1111 21 -1.0124051209671805 1111 22 -1.0073357879176044 1111 23 -1.0100299533402008 1111 24 -1.0142611898993934 1111 25 -1.0897913866593782 1112 1 0.8790468337850328 1112 2 3.3374403106057073 1112 3 1.8932049023767838 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-2.199496617335612 2315 25 -2.2097405919034188 2316 1 -0.5700952052830814 2316 2 1.7419034263503335 2316 3 0.39756967759880624 2316 4 -0.5980777230712775 2316 5 -0.8373263846697441 2316 6 -1.026441162380003 2316 7 -1.3188229634807342 2316 8 -1.2836107579204779 2316 9 -1.3742997611287464 2316 10 -1.6492109280191087 2316 11 -1.5197186928004953 2316 12 -1.7391789553375219 2316 13 -1.7268233432629927 2316 14 -1.739401765870248 2316 15 -1.7458063409976319 2316 16 -1.9791231600925059 2316 17 -1.9425571749913857 2316 18 -2.148640686674298 2316 19 -2.163185047817265 2316 20 -1.9966320165752265 2316 21 -2.170629939163554 2316 22 -1.9641480833363687 2316 23 -2.181724948710986 2316 24 -2.2401343213893057 2316 25 -2.226855176051643 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 74799.0 3 1.0 4 655.0 5 3230.0 6 3923.0 7 3644.0 8 3299.0 9 3129.0 10 3120.0 11 3140.0 12 3049.0 13 3075.0 14 3004.0 15 3069.0 16 3448.0 17 3657.0 18 4135.0 19 5104.0 20 6620.0 21 8237.0 22 10526.0 23 12619.0 24 14865.0 25 16673.0 26 19325.0 27 21894.0 28 25282.0 29 30590.0 30 38116.0 31 48478.0 32 62062.0 33 82341.0 34 111613.0 35 158117.0 36 249334.0 37 318784.0 38 751279.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.273108583904545 17.90240067807 13.035110555302246 24.789380182723214 2 17.108468410730236 20.196432156678902 39.109493050548565 23.585606382042297 3 19.75550506187578 23.162504091312417 28.951167230148382 28.13082361666342 4 12.802969961726127 15.930671883365907 37.226591544179556 34.039766610728414 5 14.04985063162296 36.993457684005 34.96118692520608 13.995504759165959 6 33.31889155231488 35.825943745163734 17.35884735668109 13.496317345840295 7 29.162922209251946 31.114123494255136 20.854625500653665 18.868328795839254 8 26.06430880942336 36.18200617149647 19.526202372866607 18.227482646213574 9 26.25988773249533 15.927597486751715 19.517783255984668 38.29473152476828 10 15.50262127785167 28.69291791455637 33.11617056941609 22.68829023817587 11 35.30509366031039 22.672965553514366 22.950512620161607 19.071428166013632 12 23.541506246227954 24.245070086783123 30.625010642142126 21.5884130248468 13 29.114299444338286 21.14134845873403 25.478281516349167 24.266070580578518 14 21.750835762894965 21.073049555489547 26.98620210799551 30.189912573619974 15 23.945623856560953 28.870097756352646 23.501870179109616 23.68240820797678 16 23.41938175303041 27.69511066881843 25.955617064509358 22.92989051364181 17 22.617721011277833 27.603257157668303 26.782629753726646 22.996392077327226 18 22.535374480426974 27.273539945398717 27.948535546646635 22.242550027527674 19 23.71874460502262 26.702471579554782 27.21197028712513 22.36681352829747 20 23.775491477772587 26.594476680938172 27.517552439746556 22.112479401542686 21 23.85577728061753 26.245299707222014 27.049185755570587 22.84973725658987 22 23.644703683242646 26.55371427057871 27.1735634445546 22.62801860162405 23 23.570967748038527 26.29886653662708 27.042920631076683 23.087245084257706 24 23.328296050344452 26.181100965597487 27.528633288163327 22.961969695894734 25 23.530580313645213 26.296733193456173 27.324243840328137 22.84844265257048 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 181.0 1 181.0 2 345.5 3 510.0 4 510.0 5 510.0 6 1403.5 7 2297.0 8 2297.0 9 2297.0 10 3404.5 11 4512.0 12 4512.0 13 4512.0 14 6671.5 15 8831.0 16 8831.0 17 8831.0 18 15194.5 19 21558.0 20 21558.0 21 21558.0 22 33063.0 23 44568.0 24 44568.0 25 44568.0 26 63203.0 27 81838.0 28 81838.0 29 81838.0 30 105130.0 31 128422.0 32 128422.0 33 128422.0 34 148073.5 35 167725.0 36 167725.0 37 167725.0 38 188280.0 39 208835.0 40 208835.0 41 208835.0 42 229550.5 43 250266.0 44 250266.0 45 250266.0 46 264488.5 47 278711.0 48 278711.0 49 278711.0 50 278916.5 51 279122.0 52 279122.0 53 279122.0 54 257162.0 55 235202.0 56 235202.0 57 235202.0 58 205829.0 59 176456.0 60 176456.0 61 176456.0 62 145851.5 63 115247.0 64 115247.0 65 115247.0 66 89380.0 67 63513.0 68 63513.0 69 63513.0 70 46516.5 71 29520.0 72 29520.0 73 29520.0 74 20431.0 75 11342.0 76 11342.0 77 11342.0 78 7668.0 79 3994.0 80 3994.0 81 3994.0 82 2566.5 83 1139.0 84 1139.0 85 1139.0 86 724.5 87 310.0 88 310.0 89 310.0 90 202.5 91 95.0 92 95.0 93 95.0 94 65.0 95 35.0 96 35.0 97 35.0 98 21.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.354386170701852 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.729840944908704E-5 20 0.0 21 2.837904566945223E-4 22 5.675809133890446E-4 23 3.7838727559269634E-4 24 4.729840944908704E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2114236.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.324258483633 #Duplication Level Percentage of deduplicated Percentage of total 1 79.90981148303045 56.19598238111018 2 13.311816596114786 18.722872623837834 3 3.6529162802596056 7.70665886136143 4 1.3991815108232646 3.935856089306216 5 0.6230733205710708 2.1908584625047753 6 0.3239829658946461 1.3670317102721485 7 0.19212331115740366 0.9457650576195294 8 0.12891082947225233 0.7252446794516966 9 0.0910727045569013 0.576415837445676 >10 0.3411644546455232 3.9402764455868184 >50 0.01413197773081552 0.6746366882214825 >100 0.009983097492838848 1.353632951622895 >500 0.0011531466762273465 0.5724772431338188 >1k 6.104894168260394E-4 0.7925764015220468 >5k 6.783215742511549E-5 0.299714567003492 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6283 0.2971759065686139 No Hit GGTATCAACGCAGAGTACTTTTTTT 3723 0.17609197837895108 No Hit TATCAACGCAGAGTACTTTTTTTTT 3606 0.17055806447340788 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.729840944908705E-5 2 0.0 0.0 0.0 0.0 4.729840944908705E-5 3 0.0 0.0 0.0 0.0 4.729840944908705E-5 4 0.0 0.0 0.0 0.0 4.729840944908705E-5 5 0.0 4.729840944908705E-5 0.0 0.0 4.729840944908705E-5 6 0.0 4.729840944908705E-5 0.0 0.0 4.729840944908705E-5 7 0.0 9.45968188981741E-5 0.0 0.0 4.729840944908705E-5 8 0.0 9.45968188981741E-5 0.0 0.0 4.729840944908705E-5 9 0.0 9.45968188981741E-5 0.0 0.0 4.729840944908705E-5 10 0.0 9.45968188981741E-5 0.0 0.0 4.729840944908705E-5 11 0.0 9.45968188981741E-5 0.0 0.0 4.729840944908705E-5 12 0.0 9.45968188981741E-5 0.0 0.0 4.729840944908705E-5 13 0.0 1.891936377963482E-4 0.0 0.0 4.729840944908705E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 65 5.6943238E-5 13.093594 5 GGTATCA 1430 0.0 11.338601 1 ACCCGTG 85 5.5795837E-5 11.125276 7 TCGAATT 60 0.006081095 11.032565 14 GTCCTAA 305 0.0 11.021248 1 TCCAACG 195 1.8189894E-12 10.668855 18 GGCGAGG 390 0.0 10.668855 19 CGGTTTC 160 5.8935257E-10 10.638545 13 GGACCGA 80 3.9353027E-4 10.638545 6 CGACTAA 80 3.9353027E-4 10.638545 17 GTCAACG 85 4.567053E-4 10.468279 2 ACGGTAC 100 2.524161E-5 10.402133 3 GTCCTAC 620 0.0 10.365096 1 TGGCGAG 715 0.0 10.183907 18 TGCCGGT 195 1.6370905E-11 10.183907 10 ACCGACT 75 0.002751024 10.086917 8 GCGACTA 85 6.8792753E-4 10.012748 16 TAGGACC 340 0.0 10.012748 4 CTTATAC 200 2.7284841E-11 9.929309 3 GCACCGT 105 4.318492E-5 9.906794 6 >>END_MODULE