FastQCFastQC Report
Thu 26 May 2016
SRR1511751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511751_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114236
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84230.39839450278966015No Hit
TATCAACGCAGAGTACTTTTTTTTT52210.24694499573368348No Hit
GGTATCAACGCAGAGTACTTTTTTT49980.23639745042653704No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24950.11800953157547217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAC250.00604315818.99434712
GGTATCA14450.012.4122521
TACACCG1103.8169674E-812.0907445
CGTTATA1052.7219357E-711.76174452
ACTGCGT1052.7319766E-711.7584078
CCGTGTA1157.1069735E-811.5617779
GTCCTAC8800.011.3832741
GGCGATT855.3408927E-511.17314612
GCGTGCG951.3652207E-510.9967289
GTCCTAA4200.010.9031641
GTAGGAC7500.010.8931843
TCCTACA9900.010.651372
GCGATTT909.5466334E-510.55241513
TAGGACG7750.010.4192124
GTATCAA32050.010.4183891
CTAGTAC1106.0483744E-610.3634953
TATACCG750.002648547310.1331955
ACCGTGT1502.456909E-810.1303198
GCGATAA856.613101E-410.05583111
CCGTTAT1053.919603E-59.9945671
Produced by FastQC (version 0.11.5)