FastQCFastQC Report
Thu 26 May 2016
SRR1511750_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511750_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629064
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65440.2489098781923909No Hit
GTCCTACAGTGGACATTTCTAAATT48600.18485666381647614No Hit
CTGTAGGACGTGGAATATGGCAAGA42360.1611219810548545No Hit
GTCCTAAAGTGTGTATTTCTCATTT41220.15678583708878902No Hit
TATCAACGCAGAGTACTTTTTTTTT39010.1483798036107147No Hit
GGTATCAACGCAGAGTACTTTTTTT38030.14465224125392154No Hit
CTTTAGGACGTGAAATATGGCGAGG36450.13864249786235708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3100.014.64221418
CCAACGA2550.013.35025319
TAGGACC8350.012.4575724
ATCCCGT2650.011.7759310
CGTTTCC2850.011.61315914
GGTATCA15250.011.5404981
TCCCGTT2800.011.48280611
GGCGAGG10800.011.47029819
AAGTCGT1900.011.4472567
CCCGTTT2850.011.28135412
CCGTTTC3050.011.16168813
CCTACAC4750.010.949553
CGCGCCT1252.3884422E-710.59120112
GTCGAGG909.97395E-510.50714319
TATACTG4250.010.4576985
CGGTAGG951.0983056E-410.4076041
TGGCGAG26000.010.329329518
GTCCTAC24500.010.2100721
AATGTCC7600.010.202998
TGTAGGA26550.010.1665252
Produced by FastQC (version 0.11.5)