FastQCFastQC Report
Thu 26 May 2016
SRR1511750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511750_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629064
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79650.3029595323658914No Hit
GTCCTACAGTGGACATTTCTAAATT55860.21247105433720898No Hit
TATCAACGCAGAGTACTTTTTTTTT50640.1926160793346986No Hit
GGTATCAACGCAGAGTACTTTTTTT47600.18105302875852394No Hit
CTGTAGGACGTGGAATATGGCAAGA46170.1756138306256523No Hit
GTCCTAAAGTGTGTATTTCTCATTT44960.1710114322055302No Hit
CTTTAGGACGTGAAATATGGCGAGG40460.15389507444474537No Hit
GTCCTACAGTGTGCATTTCTCATTT28170.107148399582513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10050.013.2336714
GGTATCA17650.011.9482311
CCAACGA2150.011.92691319
TCCAACG3450.011.83683518
GGCGAGG10850.011.3793219
GTAGGAC27350.011.0108373
CCCGTTT2450.010.85370912
GTGTCGA700.001495939210.85370813
AGGACGT25300.010.8516485
TGTAGGA28850.010.8334922
CCGTTTC2550.010.80050513
GGACGTG24950.010.7721436
GACGTGG12150.010.6303817
ATCCCGT2600.010.59280310
GGACCTG16450.010.5650466
TGCACCG909.521054E-510.5554295
ATAGGAC4250.010.5057563
AGGACCT17500.010.3684465
GAACAGT6450.010.3068176
TAGGACG27500.010.2944214
Produced by FastQC (version 0.11.5)