FastQCFastQC Report
Thu 26 May 2016
SRR1511749_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511749_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184890
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62160.2844994484848208No Hit
TATCAACGCAGAGTACTTTTTTTTT37940.17364718589951897No Hit
GGTATCAACGCAGAGTACTTTTTTT36230.16582070493251375No Hit
GTCCTACAGTGGACATTTCTAAATT24210.11080649369075789No Hit
GTCCTAAAGTGTGTATTTCTCATTT24140.11048611142895066No Hit
CTTTAGGACGTGAAATATGGCGAGG22360.10233924820013823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC350.002237659216.21166610
TATACCG400.005442053514.1852075
GCGTGCG500.001552279113.2395289
CGTTAGG600.00437675211.5303341
GGTATCA13000.011.47965051
GTCAACG1001.5499301E-510.8714582
CCCGTAC1053.6612473E-610.80802516
GGCGAGG6450.010.70329619
GTCCTAC13450.010.4342491
CCCCGTA1002.5226878E-510.40272415
GTATCAA25700.010.1138781
CGGTAGG907.7535637E-49.8831431
CCGTACA1151.0835791E-59.86819617
CGAAATC1554.3615728E-89.76186413
GTCCTAA8100.09.7611291
AGGACGT14200.09.6565975
GACGTGA7750.09.639847
TTATACT2950.09.617094
CTACACT3150.09.6069134
TGGCGAG13850.09.55944318
Produced by FastQC (version 0.11.5)