FastQCFastQC Report
Thu 26 May 2016
SRR1511749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511749_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184890
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76600.35058973220619805No Hit
TATCAACGCAGAGTACTTTTTTTTT49950.22861562824673096No Hit
GGTATCAACGCAGAGTACTTTTTTT49340.22582372567955364No Hit
GTCCTACAGTGGACATTTCTAAATT26760.12247756179944987No Hit
CTGTAGGACGTGGAATATGGCAAGA24960.11423916078154965No Hit
GTCCTAAAGTGTGTATTTCTCATTT24690.1130034006288646No Hit
CTTTAGGACGTGAAATATGGCGAGG24550.11236263610525014No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23020.10535999524003496No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG400.00529023314.24584610
TCCAACG957.4202035E-812.9962118
GGTATCA15050.012.169821
TTAGGAC9950.012.0299483
GACGTGA7850.011.8564157
GGCGAGG7600.011.12201319
GGACGTG14300.010.6262726
TAGGACC5200.010.5959754
CGTGAAA9600.010.5854559
CCGGTTT909.546384E-510.55247912
AGGACGT14600.010.475855
ACGTGAA9850.010.4132078
GTATCAA31450.010.3128261
AGTCGTC1303.8583858E-710.2277878
TACACCG750.00264867710.13316255
TCCTATA2750.010.0180122
CCTAGAC1901.0004442E-109.9998323
GTATTAT2105.456968E-129.993621
GTCCTAT2500.09.9209391
TGGCGAG14750.09.85136518
Produced by FastQC (version 0.11.5)