FastQCFastQC Report
Thu 26 May 2016
SRR1511748_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511748_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909209
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73620.38560471902238047No Hit
TATCAACGCAGAGTACTTTTTTTTT44840.23486166260477506No Hit
GGTATCAACGCAGAGTACTTTTTTT44430.23271417639451733No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19770.10355073750438008No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACGC454.990167E-415.3817512
GTCAACG653.743325E-513.6914482
TACACCG751.5507527E-512.6084225
GGTATCA13300.012.1930531
CTTATAC2050.011.5320933
GTTCTAA1551.546141E-1011.48315051
GTATCAA28150.010.9596431
GCTATTC1002.5243045E-510.4019493
TAAGGCT2400.010.2443434
GTATAAC1751.2860255E-910.1707911
GCGTGCG750.002751220310.0867389
GTATAAG2207.2759576E-129.8882681
GTGCGCC1252.7260376E-69.8345711
AAGGCGT1201.7957907E-59.4563176
TTCTAGG2154.8748916E-109.1983892
AGGCGTG1451.8162991E-69.1302377
CGTGCGC950.00190194438.95861610
TAATACC1701.8077844E-78.9000634
GGGTAAT1000.00201997238.8994411
GTCCCGC2451.8189894E-118.87735812
Produced by FastQC (version 0.11.5)