FastQCFastQC Report
Thu 26 May 2016
SRR1511748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511748_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909209
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT97740.5119397614404709No Hit
TATCAACGCAGAGTACTTTTTTTTT61850.3239561514742493No Hit
GGTATCAACGCAGAGTACTTTTTTT60450.31662327173190574No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30880.16174237603112074No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21590.11308348116942672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.012.9419321
CGCCCTA2450.012.01658116
TTAGACT1701.8189894E-1211.7358614
TTCCGAA1106.069411E-610.36033616
GTCCCGC2950.010.30180312
CCGAATG1201.5301503E-610.28838918
GCCTAAC856.3432753E-410.1013871
TCCTATA1803.929017E-1010.0282612
TAAGACT2750.010.0186644
ATAAGAC1554.060348E-89.8069253
TTAGGAC3400.09.7798833
TAGGACC1856.475602E-109.7572284
GTATAAT2350.09.7431821
GTCCTAA2900.09.5401991
TAGACAG3000.09.5004595
CTAGTAT1002.754241E-49.5004593
TAGGACG4000.09.5004594
TCCCGCC3100.09.49697513
ACTACAC1856.690243E-99.243693
GTAGGAC3400.09.2210333
Produced by FastQC (version 0.11.5)