FastQCFastQC Report
Thu 26 May 2016
SRR1511747_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511747_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669813
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47740.28590027745621815No Hit
GGTATCAACGCAGAGTACTTTTTTT29290.17540886314814894No Hit
TATCAACGCAGAGTACTTTTTTTTT27400.16409023046293209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG2800.012.1590784
TCCTATA1456.330083E-1011.5850422
TGTATCG907.847184E-611.558635
GTAGGAC2950.010.5790843
GTATCGC909.962203E-510.5078466
GTCCTAT1156.4230408E-610.3109841
CTTTATA1853.1650416E-1010.1484222
GTCCTAA2155.456968E-1210.1111571
GCGACTA750.002749118310.08753116
CGACTAA750.002749118310.08753117
TCGCGCG750.002749118310.0875319
TTATACT1606.6447683E-910.0481274
GGTATCA10950.010.0167211
ACACCGT856.873814E-410.0133586
GCACCGT856.873814E-410.0133586
AACAGCG856.873814E-410.0133587
TTAGACC951.7208618E-49.9548014
CACACCG951.7208618E-49.9548015
CCTATAC1151.0830356E-59.86823753
GTATCAA21450.09.8583261
Produced by FastQC (version 0.11.5)