##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511747_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1669813 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.96374264663169 31.0 28.0 34.0 18.0 34.0 2 26.337473717116826 31.0 25.0 34.0 10.0 34.0 3 27.978491603550818 31.0 27.0 34.0 16.0 34.0 4 32.42288627528951 35.0 32.0 37.0 23.0 37.0 5 32.860077146363096 35.0 33.0 37.0 26.0 37.0 6 33.102370145639064 37.0 35.0 37.0 27.0 37.0 7 33.12665789522539 37.0 35.0 37.0 26.0 37.0 8 33.14005041283066 37.0 35.0 37.0 26.0 37.0 9 34.69135825388831 39.0 35.0 39.0 27.0 39.0 10 34.570759120931505 39.0 35.0 39.0 27.0 39.0 11 34.58446604500025 39.0 35.0 39.0 27.0 39.0 12 34.47266370545684 39.0 35.0 39.0 27.0 39.0 13 34.465667712492355 39.0 35.0 39.0 27.0 39.0 14 35.580901574008585 40.0 36.0 41.0 27.0 41.0 15 35.50026739521132 40.0 36.0 41.0 26.0 41.0 16 35.43186752049481 40.0 36.0 41.0 26.0 41.0 17 35.39302544656198 40.0 36.0 41.0 25.0 41.0 18 35.32413390002353 40.0 36.0 41.0 25.0 41.0 19 35.34186402908589 40.0 36.0 41.0 25.0 41.0 20 35.258166633030164 40.0 36.0 41.0 25.0 41.0 21 35.17310740783549 40.0 36.0 41.0 25.0 41.0 22 35.05707046238112 40.0 36.0 41.0 24.0 41.0 23 34.94459978452677 40.0 35.0 41.0 23.0 41.0 24 34.80709576461556 40.0 35.0 41.0 22.0 41.0 25 34.69088814136673 39.0 35.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1689635996122902 1101 2 3.5950187083494214 1101 3 2.216637581374478 1101 4 1.2876062060605662 1101 5 0.831703718279087 1101 6 0.5615009811066187 1101 7 0.47693668776506115 1101 8 0.21834643804095322 1101 9 0.40213291660497674 1101 10 0.6919799508392543 1101 11 0.40873168014606875 1101 12 0.5418706105054838 1101 13 0.46020434465868476 1101 14 0.6440828772385601 1101 15 0.45365235006056537 1101 16 0.49375594286171065 1101 17 0.7454798331343184 1101 18 0.5667412425687814 1101 19 0.7602541640258238 1101 20 0.6998461474315221 1101 21 0.6495942178217007 1101 22 0.6381067488030894 1101 23 0.51957684916826 1101 24 0.7495662374713845 1101 25 0.4594071141687408 1102 1 1.4908525019647634 1102 2 4.117393783463854 1102 3 2.7557240414877846 1102 4 1.6959819290756286 1102 5 1.3277789736681598 1102 6 1.0462181922653429 1102 7 0.9386010010522199 1102 8 1.0503136572366216 1102 9 1.1418267217352565 1102 10 0.9604749172448663 1102 11 1.0113440550480846 1102 12 1.2772882714852969 1102 13 1.2474421860120515 1102 14 1.3648940883923473 1102 15 1.3786660128505446 1102 16 1.3901957041770956 1102 17 1.4153121455707947 1102 18 1.3729487240900156 1102 19 1.3161326257811368 1102 20 1.3961983679749181 1102 21 1.3769996725514986 1102 22 1.1664073944780995 1102 23 1.3378867187720758 1102 24 1.165263237592555 1102 25 1.1382343645724404 1103 1 1.5296655324609567 1103 2 4.049901008516457 1103 3 2.7508273010289273 1103 4 1.7426866820339413 1103 5 1.095910141322932 1103 6 1.0335572161763125 1103 7 1.0214286206430145 1103 8 0.8110160809242473 1103 9 1.0012686553174959 1103 10 0.9574032514329076 1103 11 1.0773036330954255 1103 12 1.0648204427394106 1103 13 1.1276438644289186 1103 14 1.2871731311867052 1103 15 1.0789765822757502 1103 16 1.1622195336706511 1103 17 1.197506644466067 1103 18 1.3761224752222958 1103 19 1.2573669475368163 1103 20 1.3009450957330202 1103 21 1.281067269328858 1103 22 1.336075593223363 1103 23 1.1265327223474557 1103 24 1.3656289235052625 1103 25 1.062630839034334 1104 1 1.6840882743224874 1104 2 4.172771251524249 1104 3 2.738678745320538 1104 4 1.8366509186226665 1104 5 1.4193956984068379 1104 6 1.1108291098030918 1104 7 1.0587426967548907 1104 8 1.1243833436477715 1104 9 1.148874340782882 1104 10 1.0243239300207065 1104 11 1.204145448776302 1104 12 1.0582940786862238 1104 13 0.9948057167902178 1104 14 1.1525990825851409 1104 15 1.1465730976356738 1104 16 1.0063350769993988 1104 17 1.04630169260912 1104 18 1.106525959862374 1104 19 1.2378538801365409 1104 20 1.1384654992176593 1104 21 1.2511901487419763 1104 22 1.3780334107382686 1104 23 1.3505510625583739 1104 24 1.2681250262104413 1104 25 1.1400136909371312 1105 1 1.5773778918088261 1105 2 3.9323340930543047 1105 3 2.494678745320538 1105 4 1.64589682026201 1105 5 1.2357563541445415 1105 6 1.0751460496937995 1105 7 1.0106224781756552 1105 8 0.8979243272543229 1105 9 0.9803278926954491 1105 10 0.8730561704578648 1105 11 0.8893585635304007 1105 12 0.9491246797791248 1105 13 0.9228931484842064 1105 14 0.9447193011643762 1105 15 0.9387916768706503 1105 16 0.9313186835567748 1105 17 0.9170885778550257 1105 18 0.8064713150536278 1105 19 0.7335587981693266 1105 20 0.8547715101466196 1105 21 1.0483814055725773 1105 22 0.8596181101918177 1105 23 1.084365270208643 1105 24 1.1941796710191852 1105 25 1.2392705215382236 1106 1 1.3437144785614095 1106 2 3.7953242380367733 1106 3 2.243407069019959 1106 4 1.4322405139983871 1106 5 0.7748812475397813 1106 6 0.7628618651017405 1106 7 0.7492186119764739 1106 8 0.5244873898905453 1106 9 0.6047086375073505 1106 10 0.5932622729879498 1106 11 0.7347177031115635 1106 12 0.7510994736765966 1106 13 0.8657691079270222 1106 14 0.9232040922190521 1106 15 1.302311055246463 1106 16 1.0700460596583596 1106 17 0.8296774151524744 1106 18 0.9815452276851033 1106 19 0.8015186200209286 1106 20 0.9308200271560025 1106 21 0.9871978558710666 1106 22 0.6815208866534874 1106 23 1.016801544253937 1106 24 1.1237011341102487 1106 25 0.8868768893957082 1107 1 1.3820041319276655 1107 2 3.8183375945663194 1107 3 2.4274942793011185 1107 4 1.4903596564867456 1107 5 1.1726060543938885 1107 6 1.1397287861784946 1107 7 0.9878753828390359 1107 8 0.917891434262657 1107 9 0.9466348585822288 1107 10 1.04695823422783 1107 11 1.1130839601031681 1107 12 1.0292520074888145 1107 13 1.017718338149443 1107 14 1.185071574494529 1107 15 1.256631350062861 1107 16 1.3001085397825705 1107 17 1.331550883547635 1107 18 1.2848754744254762 1107 19 1.3655626180006166 1107 20 1.2784396092500216 1107 21 1.4117662005219103 1107 22 1.444408815269007 1107 23 1.4600138457298897 1107 24 1.4250311750777556 1107 25 1.3060072184452238 1108 1 1.4307842629195164 1108 2 3.928853515828873 1108 3 2.5321375113559697 1108 4 1.5380730322451441 1108 5 1.135587512701278 1108 6 0.8276016612182815 1108 7 0.8748815686750717 1108 8 0.8453619630735503 1108 9 0.7852685578466136 1108 10 0.6343271880129677 1108 11 0.6917239460273734 1108 12 0.7028581185966303 1108 13 0.7627389279488384 1108 14 0.7975768875128537 1108 15 0.9105380742188558 1108 16 0.9462055718069706 1108 17 0.9039718208925649 1108 18 0.853968435936487 1108 19 0.898553941223895 1108 20 1.0112628928238507 1108 21 0.9348843047183522 1108 22 0.9782553614611302 1108 23 1.1651908507888749 1108 24 1.183139483551109 1108 25 1.3602270894303103 1109 1 1.3050813504847056 1109 2 3.2664606679436368 1109 3 2.1306431370119334 1109 4 1.465220651560351 1109 5 1.160066321552236 1109 6 0.9524413748871652 1109 7 0.7363470488814983 1109 8 0.8188699905320433 1109 9 0.9035053991409399 1109 10 0.7896315462422692 1109 11 0.7868338760760096 1109 12 0.8377738017327161 1109 13 0.7519966168891301 1109 14 0.7564428016751563 1109 15 0.7948737900194516 1109 16 0.7724122283347086 1109 17 0.886082108039389 1109 18 0.8444982645112304 1109 19 0.9243998742018178 1109 20 0.9957434418487168 1109 21 0.8688182397409818 1109 22 0.8507238162773376 1109 23 0.8315560081567881 1109 24 0.8644019797218192 1109 25 0.9590720489984577 1110 1 0.9335880798456309 1110 2 3.3951827645541997 1110 3 2.009871277409218 1110 4 1.1294257144219628 1110 5 0.5264738781034524 1110 6 0.44718461349815186 1110 7 0.2800721405510771 1110 8 0.26996327363610106 1110 9 0.04551066905981571 1110 10 0.2438050657655566 1110 11 0.15236715239357324 1110 12 0.17641698806001216 1110 13 -0.06469642245521356 1110 14 0.18999542642022504 1110 15 0.07101961649993171 1110 16 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-2.214416390133735 2315 20 -2.26388554147821 2315 21 -2.2820071174897762 2315 22 -2.216092094076892 2315 23 -2.2522808728252883 2315 24 -2.2641073440908954 2315 25 -2.248003705769918 2316 1 -1.0661705564657282 2316 2 1.3469745524651735 2316 3 -0.0851639201121337 2316 4 -1.0292981790173172 2316 5 -1.1813826449527198 2316 6 -1.269595076530397 2316 7 -2.003624417235546 2316 8 -1.7806485860144257 2316 9 -2.1403442308660487 2316 10 -2.048377288550938 2316 11 -2.153097494305605 2316 12 -2.196393257423601 2316 13 -2.1770344806492403 2316 14 -2.4715011796391337 2316 15 -2.5039751053567656 2316 16 -2.609257704120452 2316 17 -2.4698861833742214 2316 18 -2.959250686751851 2316 19 -2.6685792526723446 2316 20 -2.3788993010291364 2316 21 -2.7896295335038985 2316 22 -2.5049574885367605 2316 23 -2.76219180339875 2316 24 -2.7416068880267943 2316 25 -2.736832830734933 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 71994.0 3 3.0 4 609.0 5 3072.0 6 3587.0 7 3210.0 8 2996.0 9 2893.0 10 2868.0 11 2754.0 12 2686.0 13 2568.0 14 2686.0 15 2876.0 16 2865.0 17 3228.0 18 3683.0 19 4489.0 20 5717.0 21 7442.0 22 8964.0 23 10588.0 24 12456.0 25 14059.0 26 15651.0 27 17613.0 28 20576.0 29 24589.0 30 30373.0 31 38290.0 32 48747.0 33 63387.0 34 86610.0 35 121864.0 36 191647.0 37 243534.0 38 588639.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.785314283695236 17.56921284000065 12.552243874014636 25.09322900228948 2 17.505215640287897 20.386927296414818 38.155083794408036 23.952773268889253 3 19.6252514503121 23.39154144805436 28.862154025630417 28.121053076003122 4 12.91815311055789 15.83842023028926 36.900000179660836 34.34342647949202 5 14.138589171362304 36.946173014583074 34.83120564997398 14.084032164080648 6 33.46368725120717 35.65003985476218 17.258219932411592 13.628052961619055 7 29.233932182825264 31.16732232890749 20.95288514342624 18.645860344841008 8 26.547823019703404 35.3529407185116 19.655494357751436 18.443741904033566 9 26.57764671852477 15.566054402498963 19.2329919577821 38.623306921194164 10 15.568689428097638 28.411085552693628 33.16137795070466 22.85884706850408 11 35.58907494432012 22.439638450533085 22.790336402938532 19.180950202208273 12 23.54161813328798 24.238642291082893 30.38034797908508 21.839391596544043 13 29.309509507950892 20.97737890410483 25.27498588165262 24.438125706291665 14 22.1793098987731 20.598414313459052 26.81384083127871 30.40843495648914 15 24.20815983586186 28.338921783457188 23.32153360885321 24.131384771827744 16 23.83135117525136 27.464033397751724 25.285346323211044 23.419269103785872 17 23.014193804935044 27.20957376664333 26.521053555098682 23.25517887332294 18 22.95448652034689 26.78407701940277 27.404984869563236 22.8564515906871 19 23.950286648864274 26.554171035918394 27.01739655877634 22.47814575644099 20 24.108687619511883 26.200478736241724 27.165377200920105 22.525456443326288 21 24.302122453232787 25.907751347007117 26.726645438740743 23.06348076101935 22 23.97144806051507 26.047266766838405 26.893236850662866 23.088048321983663 23 23.92445331841739 25.87552296619599 26.723288150331598 23.47673556505502 24 23.583059899521682 26.03896364443204 27.069737749077294 23.308238706968982 25 23.93573412112614 26.02111733469556 26.846179781807905 23.1969687623704 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 254.0 1 254.0 2 368.0 3 482.0 4 482.0 5 482.0 6 1206.0 7 1930.0 8 1930.0 9 1930.0 10 2632.0 11 3334.0 12 3334.0 13 3334.0 14 4871.0 15 6408.0 16 6408.0 17 6408.0 18 11276.5 19 16145.0 20 16145.0 21 16145.0 22 24857.0 23 33569.0 24 33569.0 25 33569.0 26 46777.5 27 59986.0 28 59986.0 29 59986.0 30 76911.0 31 93836.0 32 93836.0 33 93836.0 34 110071.5 35 126307.0 36 126307.0 37 126307.0 38 143395.0 39 160483.0 40 160483.0 41 160483.0 42 177375.5 43 194268.0 44 194268.0 45 194268.0 46 206283.5 47 218299.0 48 218299.0 49 218299.0 50 220503.5 51 222708.0 52 222708.0 53 222708.0 54 207550.0 55 192392.0 56 192392.0 57 192392.0 58 168701.0 59 145010.0 60 145010.0 61 145010.0 62 121268.5 63 97527.0 64 97527.0 65 97527.0 66 76135.5 67 54744.0 68 54744.0 69 54744.0 70 40333.0 71 25922.0 72 25922.0 73 25922.0 74 18209.0 75 10496.0 76 10496.0 77 10496.0 78 7203.0 79 3910.0 80 3910.0 81 3910.0 82 2611.0 83 1312.0 84 1312.0 85 1312.0 86 831.5 87 351.0 88 351.0 89 351.0 90 225.5 91 100.0 92 100.0 93 100.0 94 67.0 95 34.0 96 34.0 97 34.0 98 20.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.295990030021326 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 8.384172359419886E-4 23 6.587563996687054E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1669813.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.69580560692887 #Duplication Level Percentage of deduplicated Percentage of total 1 81.98652330699406 60.420628840201786 2 12.200005461796636 17.98178461832071 3 3.21322936744676 7.104045805014943 4 1.1793171866540897 3.4764292054628028 5 0.5238323667873215 1.9302124136687957 6 0.27594193898841685 1.2201458096693638 7 0.15724472605557052 0.8111793734874276 8 0.10463901015183663 0.6169164920840968 9 0.07033944649033805 0.4665349957545836 >10 0.2681892868071999 3.229861721331811 >50 0.012135047270386397 0.6163194591756876 >100 0.0076199161061521236 1.0432280339708402 >500 5.73380763216001E-4 0.30043843415215155 >1k 4.095576880114293E-4 0.7822747977049894 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4774 0.28590027745621815 No Hit GGTATCAACGCAGAGTACTTTTTTT 2929 0.17540886314814894 No Hit TATCAACGCAGAGTACTTTTTTTTT 2740 0.16409023046293209 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 5.9886945424427766E-5 10 0.0 0.0 0.0 0.0 5.9886945424427766E-5 11 0.0 0.0 0.0 0.0 5.9886945424427766E-5 12 0.0 0.0 0.0 0.0 5.9886945424427766E-5 13 0.0 0.0 0.0 0.0 5.9886945424427766E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACG 280 0.0 12.159078 4 TCCTATA 145 6.330083E-10 11.585042 2 TGTATCG 90 7.847184E-6 11.55863 5 GTAGGAC 295 0.0 10.579084 3 GTATCGC 90 9.962203E-5 10.507846 6 GTCCTAT 115 6.4230408E-6 10.310984 1 CTTTATA 185 3.1650416E-10 10.148422 2 GTCCTAA 215 5.456968E-12 10.111157 1 GCGACTA 75 0.0027491183 10.087531 16 CGACTAA 75 0.0027491183 10.087531 17 TCGCGCG 75 0.0027491183 10.087531 9 TTATACT 160 6.6447683E-9 10.048127 4 GGTATCA 1095 0.0 10.016721 1 ACACCGT 85 6.873814E-4 10.013358 6 GCACCGT 85 6.873814E-4 10.013358 6 AACAGCG 85 6.873814E-4 10.013358 7 TTAGACC 95 1.7208618E-4 9.954801 4 CACACCG 95 1.7208618E-4 9.954801 5 CCTATAC 115 1.0830356E-5 9.8682375 3 GTATCAA 2145 0.0 9.858326 1 >>END_MODULE