FastQCFastQC Report
Thu 26 May 2016
SRR1511747_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511747_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669813
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57040.341595136700936No Hit
TATCAACGCAGAGTACTTTTTTTTT35810.21445515156487582No Hit
GGTATCAACGCAGAGTACTTTTTTT34990.20954442204007273No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18400.11019197958094709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.012.0263011
ACTGCGC600.005889081411.0800158
ATTATAC2250.010.555243
ACCGTGC1002.4066074E-510.4468718
TATACTG2900.010.1548685
AACACCG750.002648404610.133035
TAGCACC1606.171831E-910.0934484
GTAGGAC3800.09.9997013
CTAGACA2002.5465852E-119.97474
GGCCTAG1159.75231E-69.9556861
GTTCTAA1252.4322253E-69.9225011
GTTACAC1151.0266505E-59.9127463
TGTAGGA4800.09.8955362
GTTTAGG1356.07366E-79.8942321
CCCTTAC1553.8005965E-89.8486361
GTGTAGG2750.09.7143351
TAAAGGT2251.8189894E-129.7108214
TAGCACT2350.09.7018384
GTCCTAC3250.09.6876481
GTATCAA23050.09.6029541
Produced by FastQC (version 0.11.5)