FastQCFastQC Report
Thu 26 May 2016
SRR1511746_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511746_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315434
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51460.22224775139347525No Hit
TATCAACGCAGAGTACTTTTTTTTT31620.1365618713381595No Hit
GGTATCAACGCAGAGTACTTTTTTT31120.13440244895773318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12600.012.62931
TCCAACG1950.011.15455418
TACGCTG600.006080296411.0328335
TTATACT2750.010.66029554
TATACCG1002.5237727E-510.4023855
GTATCAA26050.010.2442461
GCCCTAT1655.340553E-910.1833091
GCGTGCG856.878272E-410.012999
CGCGCCT951.722151E-49.95443512
TAGGACG5700.09.9544354
GTCCTAC6300.09.88381
CCCTATA1751.400258E-89.6014052
GTATTAG1354.140291E-69.5177331
CTTATAC2305.456968E-129.4567153
GGCCGCT800.0046902159.4567147
TTAGGAC4050.09.3399653
AGGACGT6100.09.3016855
ATAGAAC2950.09.2964313
GTCCTAA3700.09.082411
GCGAACC1151.1675493E-49.04555311
Produced by FastQC (version 0.11.5)