##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511746_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2315434 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.812241247213265 31.0 28.0 34.0 16.0 34.0 2 26.201871009927295 31.0 25.0 34.0 10.0 34.0 3 27.834994217066864 31.0 26.0 34.0 16.0 34.0 4 32.25847594878541 35.0 32.0 37.0 20.0 37.0 5 32.68873697112507 35.0 33.0 37.0 25.0 37.0 6 32.926232403946734 36.0 35.0 37.0 26.0 37.0 7 32.94708810529689 37.0 35.0 37.0 26.0 37.0 8 32.96593338441087 37.0 35.0 37.0 26.0 37.0 9 34.4981994736192 39.0 35.0 39.0 27.0 39.0 10 34.37301430315008 39.0 35.0 39.0 26.0 39.0 11 34.37922739322304 39.0 35.0 39.0 26.0 39.0 12 34.265665529658804 39.0 35.0 39.0 25.0 39.0 13 34.25631868582737 39.0 35.0 39.0 25.0 39.0 14 35.352779219792055 40.0 36.0 41.0 25.0 41.0 15 35.27143248306797 40.0 36.0 41.0 25.0 41.0 16 35.20152204727062 40.0 36.0 41.0 25.0 41.0 17 35.16140688959392 40.0 36.0 41.0 25.0 41.0 18 35.084717595059935 40.0 36.0 41.0 24.0 41.0 19 35.10997031226111 40.0 36.0 41.0 24.0 41.0 20 35.02596662224015 40.0 35.0 41.0 23.0 41.0 21 34.92872955998746 40.0 35.0 41.0 22.0 41.0 22 34.812351377754666 40.0 35.0 41.0 21.0 41.0 23 34.68922716000542 39.0 35.0 41.0 18.0 41.0 24 34.559407437223435 39.0 35.0 41.0 18.0 41.0 25 34.43429180015496 39.0 35.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0259916683388326 1101 2 3.508646033447775 1101 3 2.0409583624485137 1101 4 1.1244589995806535 1101 5 0.7471497054065708 1101 6 0.7146290852745167 1101 7 0.5484404379578933 1101 8 0.3849166016258039 1101 9 0.5859813090596901 1101 10 0.6838996888957425 1101 11 0.48530761033158853 1101 12 0.5390530817539272 1101 13 0.4548739852747161 1101 14 0.5986184097165506 1101 15 0.5727738790067392 1101 16 0.5792723991569062 1101 17 0.6208847549208301 1101 18 0.6370383493442375 1101 19 0.6792660432793483 1101 20 0.6401853029538032 1101 21 0.8287808403176271 1101 22 0.7596597525905224 1101 23 0.7547265715643192 1101 24 0.8292308467414244 1101 25 0.7378272993961659 1102 1 1.7614382082985074 1102 2 4.373586213147611 1102 3 2.75080092936825 1102 4 1.9773799607317741 1102 5 1.5064839497450748 1102 6 1.2360712593232748 1102 7 1.1048233712521807 1102 8 1.2054386706242752 1102 9 1.3852650617502604 1102 10 1.393231848903227 1102 11 1.4200067905286105 1102 12 1.458939277280443 1102 13 1.4470717242331048 1102 14 1.5512118258740841 1102 15 1.551523107550075 1102 16 1.584335290032513 1102 17 1.637863664541186 1102 18 1.6183528301577113 1102 19 1.5575944911441297 1102 20 1.5681922554686736 1102 21 1.6113490208186576 1102 22 1.3435566178583471 1102 23 1.483061221584805 1102 24 1.3251460663231498 1102 25 1.1583822601548235 1103 1 1.6163690001146982 1103 2 4.178357577581906 1103 3 2.800990455707751 1103 4 1.6861065223822038 1103 5 1.3204384090512775 1103 6 1.1600218737303862 1103 7 1.070344529848441 1103 8 0.92764665247946 1103 9 1.1328697672853565 1103 10 0.8716305066838999 1103 11 1.1468645758667293 1103 12 1.163464607009665 1103 13 1.2753523164252414 1103 14 1.421273654958604 1103 15 1.131480502961054 1103 16 1.0336486454254725 1103 17 1.3442522168395854 1103 18 1.2580547145466454 1103 19 1.3890403285553887 1103 20 1.416213880113311 1103 21 1.3744119684192881 1103 22 1.4635087313877335 1103 23 1.3574456493550784 1103 24 1.6223849238832315 1103 25 1.2634237384602756 1104 1 1.6571830090004376 1104 2 4.0075852736793465 1104 3 2.6450985765921864 1104 4 1.82891008147827 1104 5 1.4977697610055145 1104 6 1.2681349102076993 1104 7 1.040563598729527 1104 8 1.1131022588774826 1104 9 1.2888380486005673 1104 10 1.111071768857471 1104 11 1.1958254597870948 1104 12 1.1806516395943945 1104 13 1.0449952988716618 1104 14 1.4074261602121254 1104 15 1.4727201944346646 1104 16 1.2423406766991292 1104 17 1.3415499795246575 1104 18 1.1863298991122022 1104 19 1.4053142279020676 1104 20 1.424914036333881 1104 21 1.4121020275467373 1104 22 1.5687048657595852 1104 23 1.3769612178613002 1104 24 1.3202530024440833 1104 25 1.414449420678828 1105 1 1.377843040647516 1105 2 3.84335277841425 1105 3 2.4064146142048415 1105 4 1.5701562505514843 1105 5 1.130637739365902 1105 6 0.8183302953537464 1105 7 0.7980311478640019 1105 8 0.6924874373448588 1105 9 0.8610985813414231 1105 10 0.6067714043846948 1105 11 0.7352251289066913 1105 12 0.8292886717214998 1105 13 0.6891248285513782 1105 14 0.6083459428793603 1105 15 0.7010272197442191 1105 16 0.9257809412593545 1105 17 0.9332483848324316 1105 18 1.0167554956020268 1105 19 0.8028165041682911 1105 20 0.838555287771463 1105 21 0.9409903450550345 1105 22 0.9133729992032684 1105 23 0.9496153127539131 1105 24 0.9481870443093712 1105 25 0.9557293172179087 1106 1 1.375284139822213 1106 2 3.9641974462182077 1106 3 2.5281929350126227 1106 4 1.6296218782890506 1106 5 0.9881115740286361 1106 6 0.9536984543283396 1106 7 0.8915808735313959 1106 8 0.7673179966145369 1106 9 0.7242319669285919 1106 10 0.7889835856747496 1106 11 1.0180528295844553 1106 12 0.7118443451043177 1106 13 1.0354409334893688 1106 14 1.0760945712163021 1106 15 1.078084714326728 1106 16 0.9423907434632355 1106 17 0.9695455861787394 1106 18 1.0741578654669297 1106 19 0.9272574650240202 1106 20 1.1279761923774885 1106 21 0.9484941488265264 1106 22 0.9137649029332735 1106 23 0.935760363240334 1106 24 1.041702190235874 1106 25 0.9428887067702192 1107 1 1.444483287694549 1107 2 3.813949157098218 1107 3 2.5507084186566047 1107 4 1.7171294644186048 1107 5 1.3083246456431823 1107 6 1.1980837259973782 1107 7 0.9302784461188054 1107 8 0.8305787495654968 1107 9 0.9342542427327203 1107 10 0.9222203359381282 1107 11 1.0240907187969412 1107 12 0.838957854444125 1107 13 0.8776129658178178 1107 14 0.9903917767913555 1107 15 1.0720130814145108 1107 16 1.183849245411686 1107 17 1.1040616196465294 1107 18 1.0098004422747877 1107 19 1.2324870933818346 1107 20 1.2542257637580647 1107 21 1.4108867954788806 1107 22 1.237676631399701 1107 23 1.3038232416473932 1107 24 1.4009026332708032 1107 25 1.2837888993574254 1108 1 1.5256497735295902 1108 2 3.941462919782417 1108 3 2.575547952098617 1108 4 1.5369377347086726 1108 5 1.2326958616536174 1108 6 1.036439869447321 1108 7 0.8385445647424277 1108 8 0.8414003556890179 1108 9 0.6554298396732676 1108 10 0.6352494233283323 1108 11 0.7504368474650818 1108 12 0.7419196245592872 1108 13 0.8993730612407447 1108 14 0.8240562791230346 1108 15 0.7523347042651309 1108 16 0.896062009137097 1108 17 0.8619707489659945 1108 18 0.8796260385338641 1108 19 0.787074634130903 1108 20 0.8121015214852605 1108 21 0.9221046892605713 1108 22 0.9789916538615131 1108 23 1.1002342260226285 1108 24 0.9738875033012562 1108 25 1.1886864696329127 1109 1 1.0644731565792576 1109 2 2.9879022969697466 1109 3 1.9217334691362247 1109 4 1.1798980179032696 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-2.281359584754 2315 20 -2.330487647808731 2315 21 -2.303958922036024 2315 22 -2.1663581998438843 2315 23 -2.197919389483907 2315 24 -2.138766415988897 2315 25 -2.3294696796607113 2316 1 -0.9249967672653732 2316 2 1.494397247371488 2316 3 0.1914013264427048 2316 4 -0.7765380380023288 2316 5 -1.1678765613801332 2316 6 -1.2130291999812535 2316 7 -1.7473158124623112 2316 8 -1.5840301707449456 2316 9 -1.8267030458365525 2316 10 -1.9077964780928482 2316 11 -1.9240454787392451 2316 12 -2.113201184313617 2316 13 -2.023986799323474 2316 14 -2.2139154063386 2316 15 -2.1916658608882926 2316 16 -2.2488278812120015 2316 17 -2.1587167097577264 2316 18 -2.520027717054276 2316 19 -2.325658231504754 2316 20 -2.1590315378502822 2316 21 -2.479404721804414 2316 22 -2.4415163510618925 2316 23 -2.5573132836528387 2316 24 -2.509584836864825 2316 25 -2.5507836638552703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 107403.0 3 3.0 4 952.0 5 4588.0 6 5277.0 7 4785.0 8 4429.0 9 4298.0 10 3955.0 11 3935.0 12 3995.0 13 3880.0 14 3938.0 15 4101.0 16 4181.0 17 4553.0 18 5190.0 19 6536.0 20 8272.0 21 10613.0 22 12812.0 23 15393.0 24 17408.0 25 20091.0 26 22343.0 27 25278.0 28 29045.0 29 35028.0 30 43249.0 31 54461.0 32 68812.0 33 89589.0 34 120725.0 35 170870.0 36 265123.0 37 336585.0 38 793738.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0344229202819 17.98060320441006 12.770262508022254 25.214711367285787 2 17.33298364989164 20.74840080764236 38.15148665868895 23.76712888377705 3 19.104625741869558 23.876776448821257 29.155052573297276 27.86354523601191 4 12.845324029965873 16.362763956994673 37.19510035699571 33.59681165604375 5 14.13091454992887 36.90457166993315 34.93176657162329 14.03274720851469 6 32.81881496082376 35.999428184953665 17.702210471125497 13.479546383097077 7 28.716430699385082 31.212679782710286 21.617156869943173 18.453732647961463 8 26.122446159121786 35.405500653441216 20.178333737865124 18.293719449571874 9 26.609223152117483 15.468979033736224 19.518975708225756 38.40282210592053 10 15.576518268281452 28.41467301594431 33.32489718990047 22.683911525873768 11 35.23460396625428 22.43946491240951 23.30034023859026 19.025590882745956 12 23.516368853528107 24.147524826879106 30.701371751472944 21.634734568119843 13 29.12827573577999 21.11763928490296 25.43384955045145 24.3202354288656 14 22.12025909613489 20.76487604483652 26.95516261746178 30.159702241566812 15 24.036400951182372 28.444473044794194 23.407922661583097 24.111203342440337 16 23.75623749154586 27.375731720273606 25.51085455253745 23.357176235643077 17 22.97919094217326 27.188855307471517 26.62325939759026 23.208694352764965 18 22.885817518443627 26.93525274311425 27.484221100666222 22.6947086377759 19 24.018757622767588 26.521012061680064 26.806531135442548 22.6536991801098 20 24.152577875249307 26.340591007992455 27.01385571776177 22.492975398996474 21 24.391797635860293 25.91142897863464 26.53584863286733 23.160924752637737 22 24.042905409344918 26.196684055887943 26.648438144456794 23.11197239031035 23 23.95844040524794 26.002214715071375 26.583966119351032 23.45537876032965 24 23.676153597321623 26.11418781211792 26.877587801148035 23.332070789412423 25 23.837906845973585 26.072693067476767 26.801454932423034 23.287945154126614 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 280.0 1 280.0 2 414.5 3 549.0 4 549.0 5 549.0 6 1399.0 7 2249.0 8 2249.0 9 2249.0 10 3126.0 11 4003.0 12 4003.0 13 4003.0 14 6496.5 15 8990.0 16 8990.0 17 8990.0 18 15931.0 19 22872.0 20 22872.0 21 22872.0 22 35134.5 23 47397.0 24 47397.0 25 47397.0 26 67622.5 27 87848.0 28 87848.0 29 87848.0 30 113213.0 31 138578.0 32 138578.0 33 138578.0 34 160312.0 35 182046.0 36 182046.0 37 182046.0 38 205090.5 39 228135.0 40 228135.0 41 228135.0 42 249671.5 43 271208.0 44 271208.0 45 271208.0 46 285296.0 47 299384.0 48 299384.0 49 299384.0 50 300585.0 51 301786.0 52 301786.0 53 301786.0 54 280980.0 55 260174.0 56 260174.0 57 260174.0 58 228356.0 59 196538.0 60 196538.0 61 196538.0 62 163626.0 63 130714.0 64 130714.0 65 130714.0 66 102499.5 67 74285.0 68 74285.0 69 74285.0 70 55055.5 71 35826.0 72 35826.0 73 35826.0 74 25214.5 75 14603.0 76 14603.0 77 14603.0 78 10014.5 79 5426.0 80 5426.0 81 5426.0 82 3623.5 83 1821.0 84 1821.0 85 1821.0 86 1179.5 87 538.0 88 538.0 89 538.0 90 337.0 91 136.0 92 136.0 93 136.0 94 87.0 95 38.0 96 38.0 97 38.0 98 24.0 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.323034040270636 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 8.637689521705218E-5 20 0.0 21 1.7275379043410436E-4 22 6.478267141278914E-4 23 5.182613713023131E-4 24 1.2956534282557828E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2315434.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.46005210826692 #Duplication Level Percentage of deduplicated Percentage of total 1 81.38515623443668 58.97172661586727 2 12.500657912844934 18.115966475047262 3 3.3174812182173317 7.21154585820674 4 1.2400663031367465 3.5942107577197833 5 0.5752338897192097 2.0840738811747506 6 0.2857456099482436 1.2423085071934947 7 0.18229501709218332 0.9246374506303908 8 0.10458313495705941 0.6062479526907549 9 0.06949320645737413 0.4531933224964692 >10 0.3163645582234704 3.7607197815881075 >50 0.012930220917246592 0.6398217639532985 >100 0.008490179836973972 1.1516167609221746 >500 0.0011419108015098694 0.5696196545463397 >1k 3.0050284250091947E-4 0.4502085618253572 >5k 6.01005685001839E-5 0.2241026561378688 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5146 0.22224775139347525 No Hit TATCAACGCAGAGTACTTTTTTTTT 3162 0.1365618713381595 No Hit GGTATCAACGCAGAGTACTTTTTTT 3112 0.13440244895773318 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.318844760852609E-5 0.0 0.0 0.0 3 0.0 4.318844760852609E-5 0.0 0.0 0.0 4 0.0 4.318844760852609E-5 0.0 0.0 0.0 5 0.0 4.318844760852609E-5 0.0 0.0 0.0 6 0.0 4.318844760852609E-5 0.0 0.0 0.0 7 4.318844760852609E-5 4.318844760852609E-5 0.0 0.0 0.0 8 4.318844760852609E-5 4.318844760852609E-5 0.0 0.0 0.0 9 4.318844760852609E-5 4.318844760852609E-5 0.0 4.318844760852609E-5 0.0 10 4.318844760852609E-5 4.318844760852609E-5 0.0 4.318844760852609E-5 0.0 11 4.318844760852609E-5 4.318844760852609E-5 0.0 4.318844760852609E-5 0.0 12 4.318844760852609E-5 4.318844760852609E-5 0.0 4.318844760852609E-5 4.318844760852609E-5 13 4.318844760852609E-5 4.318844760852609E-5 0.0 4.318844760852609E-5 4.318844760852609E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1260 0.0 12.6293 1 TCCAACG 195 0.0 11.154554 18 TACGCTG 60 0.0060802964 11.032833 5 TTATACT 275 0.0 10.6602955 4 TATACCG 100 2.5237727E-5 10.402385 5 GTATCAA 2605 0.0 10.244246 1 GCCCTAT 165 5.340553E-9 10.183309 1 GCGTGCG 85 6.878272E-4 10.01299 9 CGCGCCT 95 1.722151E-4 9.954435 12 TAGGACG 570 0.0 9.954435 4 GTCCTAC 630 0.0 9.8838 1 CCCTATA 175 1.400258E-8 9.601405 2 GTATTAG 135 4.140291E-6 9.517733 1 CTTATAC 230 5.456968E-12 9.456715 3 GGCCGCT 80 0.004690215 9.456714 7 TTAGGAC 405 0.0 9.339965 3 AGGACGT 610 0.0 9.301685 5 ATAGAAC 295 0.0 9.296431 3 GTCCTAA 370 0.0 9.08241 1 GCGAACC 115 1.1675493E-4 9.045553 11 >>END_MODULE